Analisis Sidik Jari DNA Plasma Nutfah Kedelai Menggunakan Markah SSR

Abstract

<p class="p1">Accuracy is an important issue for plant germplasm identification, especially forvarietal conformation, registration, and plant protection. A study was conducted to determine genetic variation in 96 soybean accessions based on variation in size and number alelles using fluorescently-labeled SSR (Simple Sequence Repeat) markers on a capillary-electrophoresis DNA analyzer. This technology can be used to measure sizes of DNA fragments accurately and the genotyping protocol can be automated in a high-throughput manner. In addition, the germplasm as a whole can be further analyzed to measure the amount of genetic diversity and to identify agronomically-important genes or alleles for variety improvement. Results of the study indicated that nearly all the soyben accessions tested showed unique DNA fingerprints or genetic identities. The rare alleles (frequency <5%) that might have the potential in the variety improvement program had also been detected. Identification of the 96 soybean accessions using 10 SSR markers had detected 116 alleles, ranging between 7-19 alleles per locus, with the value of PIC (Polymorphism Information Content), reflecting the value of frequency and allele variation) 0.703. The tendency for clustering together of the allelles in certain groups of the improved soyben varieties indicating that there were close genetic relationships among them. In addition, molecular differences between two accessions having the same names but with different number of registrations were detected. Furthermore, the presence of two soybean accessions with different names but having the same molecular identity was also identified.</p>

Genetic Diversity Analysis of Aluminum-toxicity Tolerant and Sensitive Soybean Genotypes Assessed with Microsattelite Markers

“…The high allelic diversity and polymorphism level observed in this study are generally very similar to the ones previously reported (Akkaya et al 1992, Diwan et al 1997, Santoso et al 2006, Trustinah et al 2006). This finding also further indicated that the polymorphism level of SSR markers is much higher than that shown by RFLP markers that generally show polymorphism level ranges from 20 to 30% (Cregan et al 1999, Tasma et al 2001.…”

“…It is possible that the two genotypes were actually the same genetically but they were given different names as they were collected from different areas although both regions were actually neighbours. Similar observation was reported by Santoso et al (2006) showing that some soybean accessions with different names were genetically identical based on SSR marker genotyping. This information becomes crucial to soybean curators to combine the identical accessions for efficient storage and handlings.…”

“…SSR markers demonstrate high levels of length polymorphism in soybean. Santoso et al (2006) and Cregan 1997). The SSR marker has also been successfully used in the construction of the soybean genetic linkage map (Cregan et al 1999, Tasma et al 2001, Song et al 2004.…”

Pra-pemuliaan Aneka Kacang dalam Mendukung Proses Pemuliaan untuk Perakitan Varietas Unggul Baru

“…Marka SSR kacang hijau berbasis kedelai asal Korea telah berhasil membedakan varietas lokal kacang hijau asal Indonesia yang berasal dari daerah kering (Nusa Tenggara) (Lestari et al, 2014a). Marka molekuler telah dimanfaatkan untuk mengidentifikasi keragaman genetik plasma nutfah kedelai Indonesia (Chaerani et al, 2011;Santoso et al, 2006) dan terkait ketahanan terhadap keracunan aluminium (Tasma et al, 2009). Namun, pemetaan genetik/QTL aneka kacang sebagai bagian dari pra-pemuliaan masih terbatas implementasinya di Indonesia.…”

Analisis Keragaman Genetik Kedelai Introduksi Menggunakan Marka Mikrosatelit

“…Karakterisasi molekuler memiliki keunggulan, yaitu waktu yang dibutuhkan lebih cepat karena tidak perlu menunggu tanaman hingga fase dewasa, tidak bersifat destruktif, mampu membedakan individu berkerabat dekat, dan tidak dipengaruhi oleh faktor lingkungan (Pandin, 2009;Yono et al, 2017). Informasi karakterisasi molekuler dapat digunakan bersama dan saling melengkapi dengan data karakter morfologi (Santoso et al, 2006). Mikrosatelit atau Simple Sequence Repeat (SSR) merupakan salah satu penanda molekuler yang banyak dimanfaatkan dalam karakterisasi secara molekuler.…”