Análisis de diversidad genética en cerdo criollo san pedreño utilizando datos de pedigrí

Estany

Rondón‐Barragán

2021

Trop Anim Health Prod

BackgroundColombian Creole pigs are a valuable resource for rural livelihood and research. There are three o cially recognized Creole breeds in Colombia (Zungo, ZU; Casco de Mula, CM; and San Pedreño, SP). The phenotypic characterization of these breeds is still very limited, including the reproductive performance of their boars, which is a key issue for developing conservation and dissemination strategies. The aim of this research was to assess the semen quality of Colombian Creole breeds as compared to commonly used international breeds. ResultsSeven boars for each Colombian Creole (ZU, CM, and SP) and international (Duroc, Belgian Landrace, and Pietrain) breeds were used in the experiment. Two doses of semen per boar were assessed in duplicate for sperm kinetics and membrane and acrosome integrity using computer-assisted sperm analysis and ow cytometry, respectively. On average, the Creole pigs, as compared to international breeds, showed lower (P<0.05) volumeof uid ejaculated(185.5 mL vs 239.9 mL) as well as sperm concentration (340.5 vs to 395.4, in million sperm/mL), motility (90.9% vs 95.3%) and progressive motility (63.1% vs 67.2%).No relevant differences between breeds for sperm velocity traits were observed, but Creole pigs had lower (P<0.05) proportion of morphologic normal sperm (86.1% vs 90.6%) and of sperm with intact mitochondria plasma membrane and acrosome (76.8% vs 87.5%). Mitochondrial membrane potential did not differ between Creole and international breeds. These results mean that Creole breeds had 60.5% less normal and motile sperm per ejaculate than international breeds. Amongst Creole breeds, SP had larger ejaculates and ZU showed greater proportion of normal and motile sperm, but they did not differ for the amount of normal and motile sperm per ejaculate. ConclusionThe semen of Colombian Creole pigs is acceptable but less abundant and rich in normal and motile spermatozoa than that collected from commercial breeds. This fact should be considered in developing recommendations for semen processing in Creole pigs. Findings provided here can give new impetus to the conservation and insemination of Creole pigs.

Section: Animals and Semen Collectionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Semen quality of Colombian Creole as compared to commercial pig breeds

Estany

Rondón‐Barragán

2021

Trop Anim Health Prod

“…The integrity of the sperm plasmatic membrane (namely, viability) was assessed in samples diluted to 30x10 6 spermatozoa/mL stained with propidium iodide (IP) at 12 μM. Acrosome integrity was assessed in the same samples using the double stain PNA-FITC (peanut agglutinin-uorescein isothiocyanate) and IP (12).…”

Section: Plasma Membrane and Acrosome Integritymentioning

confidence: 99%

Semen quality of Colombian Creole as compared to commercial pig breeds

Estany

Rondón‐Barragán³

2020

Preprint

Background Colombian Creole pigs are a valuable resource for rural livelihood and research. There are three officially recognized Creole breeds in Colombia (Zungo, ZU; Casco de Mula, CM; and San Pedreño, SP). The phenotypic characterization of these breeds is still very limited, including the reproductive performance of their boars, which is a key issue for developing conservation and dissemination strategies. The aim of this research was to assess the semen quality of Colombian Creole breeds as compared to commonly used international breeds. Results Seven boars for each Colombian Creole (ZU, CM, and SP) and international (Duroc, Belgian Landrace, and Pietrain) breeds were used in the experiment. Two doses of semen per boar were assessed in duplicate for sperm kinetics and membrane and acrosome integrity using computer-assisted sperm analysis and flow cytometry, respectively. On average, the Creole pigs, as compared to international breeds, showed lower (P<0.05) volumeof fluid ejaculated(185.5 mL vs 239.9 mL) as well as sperm concentration (340.5 vs to 395.4, in million sperm/mL), motility (90.9% vs 95.3%) and progressive motility (63.1% vs 67.2%). No relevant differences between breeds for sperm velocity traits were observed, but Creole pigs had lower (P<0.05) proportion of morphologic normal sperm (86.1% vs 90.6%) and of sperm with intact mitochondria plasma membrane and acrosome (76.8% vs 87.5%). Mitochondrial membrane potential did not differ between Creole and international breeds. These results mean that Creole breeds had 60.5% less normal and motile sperm per ejaculate than international breeds. Amongst Creole breeds, SP had larger ejaculates and ZU showed greater proportion of normal and motile sperm, but they did not differ for the amount of normal and motile sperm per ejaculate. Conclusion The semen of Colombian Creole pigs is acceptable but less abundant and rich in normal and motile spermatozoa than that collected from commercial breeds. This fact should be considered in developing recommendations for semen processing in Creole pigs. Findings provided here can give new impetus to the conservation and insemination of Creole pigs.

“…Still, there is a growing interest in the conservation of local breeds in favour of biodiversity (Ciobanu et al, 2001) and, in particular, as a source of adaptive variation against climate change disturbances. The three officially recognized Colombian Creole (CR) pig breeds (ZU, Zungo; CM, Casco de Mula; and SP, San Pedreño) are a good example of environmental adaptation, with pigs living in areas from around sea level to 3000 m of altitude (Ocampo-Gallego, 2019;Suárez-Mesa et al, 2021). Similar to other local breeds (Kušec et al, 2015), the census of CR pigs has been continuously declining in recent decades as intensive farming has replaced traditional production systems.…”

Section: Introductionmentioning

confidence: 99%

“…Currently, most of these individuals are maintained in three independent nucleus farms, one per breed, which are managed by the Colombian Agricultural Research Corporation (AGROSAVIA). In each nucleus farm, pigs are distributed in family groups and subjected to a circular mating system for maintaining genetic variability (Ocampo-Gallego, 2019). These pigs are amongst the few available individuals that can be used to investigate whether CR pigs harbour specific genetic variants that merit conservation.…”

Section: Introductionmentioning

confidence: 99%

Identification of breed-specific genomic variants in Colombian Creole pig breeds by whole-genome sequencing

Ros‐Freixedes

Laghouaouta

et al. 2023

Trop Anim Health Prod

Dissecting genetic variation of local breeds is important for the success of conservation. In this research, we investigated the genomic variation of Colombian Creole (CR) pigs, with a focus on the breed-specific variants in the exonic region of 34 genes with reported effects on adaptive and economic traits. Seven individuals of each of the three CR breeds (CM, Casco de Mula; SP, San Pedreño; and ZU, Zungo) were whole-genome sequenced along with 7 Iberian (IB) pigs and 7 pigs of each of the four most used cosmopolitan (CP) breeds (Duroc, Landrace × Large White, and Pietrain). Molecular variability in CR (6,451,218 variants; from 3,919,242, in SP, to 4,648,069, in CM) was comparable to that in CP, but higher than in IB. For the investigated genes, SP pigs displayed less exonic variants (178) than ZU (254), CM (263), IB (200), and the individual CP genetic types (201 to 335). Sequence variation in these genes confirmed the resemblance of CR to IB and indicates that CR pigs, particularly ZU and CM, are not exempt from selective introgression of other breeds. A total of 50 exonic variants were identified as being potentially specific to CR, including a high-impact deletion in the intron between exons 15 and 16 of the leptin receptor gene, which was only found in CM and ZU. The identification of breed-specific variants in genes related to adaptive and economical traits can bolster the understanding of the role of gene-environment interactions on local adaptation and points the way for effective breeding and conservation of CR pigs.