Análises microbiológicas de alicates ortodônticos

Abstract

Objective: To evaluate bacterial contamination of orthodontic pliers used in an academic setting. Methods: Thirty-four pliers were selected -17 band remover pliers and 17 bird beak pliers. The control group was composed of 3 previously autoclaved pliers of each model. After use, the pliers in the experimental group were immersed in 10 ml of brain-heart infusion (BHI) culture medium for 2 minutes, incubated at 37º C for 24 to 48 h and seeded in duplicates in different agar-based solid culture media to detect and identify microbial agents. Results: Microbiological analyses revealed that there was contamination in both types of orthodontic pliers. Several bacteria were detected, predominantly staphylococcus and isolated Gram-positive (G+) cocci. The band remover pliers had a greater contamination rate and mean values of 2.83 x 10 9 and 6.25 x 10 9 CFU/ml, with variations according to the type of culture medium. The 139 pliers also had all types of bacteria from the oral microbiota at values that ranged from 1.33 x 10 8 to 6.93 x 10 9 CFU/ml. The highest mean value was found in the medium to grow staphylococci, which confirmed, in certain cases, the presence of Staphylococcus aureus, which are not part of the normal oral microbiota but are usually found in the nasal cavity and on the skin. Conclusion: Orthodontic pliers were contaminated as any other dental instrument after use in clinical situations. Therefore, they should undergo sterilization after each use in patients. Abstract

In vitro evaluation of microbial contamination and the disinfecting efficacy of chlorhexidine on orthodontic brackets

“…Bacterial colonization was confirmed in all the orthodontic brackets received from different manufacturers. The outcome of the current investigation was similar to prior studies using different orthodontic appliances, such as arch wires [1], orthodontic pliers [6], brackets [11], orthodontic buccal tubes [4], and tooth brushes [13] received from different manufacturers. These studies indicate that orthodontic appliances used in dentistry are often contaminated with bacteria.…”

“…In our study, Staphylococci were the predominant organisms isolated from orthodontic brackets. Contamination with Staphylococci mostly occurs due to skin contact during manufacturing and/or packaging of orthodontic appliances [1, 6]. Similar studies conducted in this regard reported that Staphylococci were the common organisms to contaminate the orthodontic brackets [1, 3, 14].…”

“…Pathogens involved in transmitting the infection include viruses, such as hepatitis B and C, herpes simplex, and human immunodeficiency. Further, bacterial contaminations due to Mycobacterium tuberculosis , Staphylococcal and Streptococcal spp., and other microorganisms are responsible for the upper respiratory tract infections [6]. Among all of these, gram-positive Staphylococci are considered as the major cause of nosocomial infections.…”

Prevention and Control Measures in Dentistry Under Covid-19 Conditions

“…Відомо, що ротова порожнина містить свій мікробіом, до якого може входити різноманітна патогенна флора, як наприклад Streptococcus mutans, Streptococcus sanguinis, Staphylococcus aureus та Porphyromonas gingivalis [1]. Наслідком використання застарілих методів стерилізації є збільшення захворюваності на інфекції верхніх дихальних шляхів, в патогенезі яких суттєву роль відіграють Mycobacterium tuberculosis, а також деякі бактеріальні штами роду Staphylococcus and Streptococcus [3].…”

“…Відповідно до сучасних протоколів інструменти класифікують згідно трьох категорій за ризиком інфікування. Критична позиція передбачає використання стерилізаційних процедур для усіх категорій інструментів, через їх контакт із біологічними рідинами та тканинами [3].…”