DOI: 10.11606/d.41.2010.tde-09122010-163822
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Análise da variação genética de populações de Vochysia pyramidalis e V. tucanorum por AFLP e da composição de ácidos graxos de sementes

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Cited by 5 publications
(4 citation statements)
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References 71 publications
(62 reference statements)
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“…Thus, to improve DNA isolation from endocarps of dispersed seeds, we added detergent, sarcosyl, and β-mercaptoethanol to the extraction buffer. These reagents solubilize membranes and assist in the inactivation and denaturation of some enzymes such as peroxidases and polyphenolodidases (Clemente 2010). After the addition of these reagents, the success of DNA extraction from dispersed endocarps increased but, the same success was not observed for microsatellite amplification.…”
Section: Resultsmentioning
confidence: 99%
“…Thus, to improve DNA isolation from endocarps of dispersed seeds, we added detergent, sarcosyl, and β-mercaptoethanol to the extraction buffer. These reagents solubilize membranes and assist in the inactivation and denaturation of some enzymes such as peroxidases and polyphenolodidases (Clemente 2010). After the addition of these reagents, the success of DNA extraction from dispersed endocarps increased but, the same success was not observed for microsatellite amplification.…”
Section: Resultsmentioning
confidence: 99%
“…Several investigations have related the activity of these psychotogens to alterations of central adrenergic a d tryptaminergic systems (Roberts & Straughan, 1968;Sugrue, 1969;Tilson & Sparber, 1972). To the premise that mescaline elicits central mtecholaminergic antagonism as suggested by various authors (Speck, 1957;Clemente & Lynch, 1968;Gowalez-Vegas, 1971 ;Ratcliffe, 1971 ;Dill, 1972), studies were conducted to observe the interaction of mescaline with endogenously released noradrenaline.…”
mentioning
confidence: 99%
“…Esse material passou por um processo de transmetilação como descrito em Clemente (2010), com o intuito de tornar os ácidos graxos mais voláteis durante a análise em cromatógrafo a gás. Para tanto, 30 μL das amostras solubilizadas em tolueno foram transferidas para tubos de vidro com rosca e a eles foram adicionados 4 mL da solução de H 2 SO 4 a 5% em metanol e 2 mL de tolueno.…”
Section:  Análise Qualitativa De Lipídeosunclassified