Degraded areas undergoing recovery need to be assessed and monitored through environmental quality indicators in order to describe the real condition of the ecosystem. Therefore, the understanding the microbiological activity associated with the formation of aggregates is a fundamental in assessing soil quality. Thus, the aim this study is to quantify the fractions of glomalin-related soil protein easily extractable (GRSP-EE) and total (GRSP-T), evaluating its relationship with the physical properties of soil aggregates through morphometric analysis. Mined areas submitted to forest restoration in the brazilian Amazon were selected at different land reclamation stages, from 0 to 30 years. The reference ecosystem (Native Forest > 100 years) was also evaluated, providing the identification of association's among physical and microbiological soil properties. The results demonstrate higher concentration of GRSP (T and EE) in advanced stage locations, at the age of 30 years, when compared to the youngest forests, 10 year old. Furthermore, the macroaggregates showed higher GRSP highlighting as fundamental for the maintenance of aggregate stability. All parameters analyzed (GRSP, aggregate diameter, revegetation age and stage of development) allowed the significance differentiations of reclamation stages. The GRSP-EE, GRSP-T and circularity of soil aggregates showed positive and significant correlations, allowing the identification of associations between soil glomalin and soil aggregates morphometry. The glomalin showed association with soil aggregation, allowing the identification of soil structure improvement with the advance of land reclamation and a promising ecosystem quality indicator.