Análise comparativa entre as sensibilidades da reação indireta de anticorpos fluorescentes e da reação de Sabin-Feldman na pesquisa de anticorpos séricos para toxoplasmose

Coutinho, S.; Andrade, Carlos Maurício de; Malvar, Gilsea Sarmento; Ferreira, Luiz Fernando

doi:10.1590/s0037-86821970000500003

Rev. Soc. Bras. Med. Trop.

1970

DOI: 10.1590/s0037-86821970000500003

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Análise comparativa entre as sensibilidades da reação indireta de anticorpos fluorescentes e da reação de Sabin-Feldman na pesquisa de anticorpos séricos para toxoplasmose

S. Coutinho¹,

Carlos Maurício de Andrade²,

Gilsea Sarmento Malvar³

et al.

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Cited by 8 publications

(1 citation statement)

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“…We conducted a population case-control study to investigate the role of the (*147570 interferon, gamma; IFN-γ) IFN-γ +874T/A SNP in retinochoroiditis susceptibility. Toxoplasmosis diagnosis -Indirect immunofluorescence (IIF) and Enzyme Linked Immunosorbent Assays (ELISA) were performed as described by Coutinho et al (1970) and Uchôa et al (1999), respectively. Serum was considered positive if it showed titer ≥ 1/16 (IIF) or an ELISA score of 1.4-fold higher than the ELISA cut-off.…”

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confidence: 99%

The IFN-³+874T/A gene polymorphism is associated with retinochoroiditis toxoplasmosis susceptibility

Albuquerque

Aleixo²,

Benchimol³

et al. 2009

Mem. Inst. Oswaldo Cruz

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confidence: 99%

The IFN-³+874T/A gene polymorphism is associated with retinochoroiditis toxoplasmosis susceptibility

Albuquerque

Aleixo²,

Benchimol³

et al. 2009

Mem. Inst. Oswaldo Cruz

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Toxoplasmosis in humans and animals in Brazil: high prevalence, high burden of disease, and epidemiology

et al. 2012

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SUMMARYInfections by the protozoan parasiteToxoplasma gondiiare widely prevalent in humans and animals in Brazil. The burden of clinical toxoplasmosis in humans is considered to be very high. The high prevalence and encouragement of the Brazilian Government provides a unique opportunity for international groups to study the epidemiology and control of toxoplasmosis in Brazil. Many early papers on toxoplasmosis in Brazil were published in Portuguese and often not available to scientists in English-speaking countries. In the present paper we review prevalence, clinical spectrum, molecular epidemiology, and control ofT. gondiiin humans and animals in Brazil. This knowledge should be useful to biologists, public health workers, veterinarians, and physicians. Brazil has a very high rate ofT. gondiiinfection in humans. Up to 50% of elementary school children and 50–80% of women of child-bearing age have antibodies toT. gondii. The risks for uninfected women to acquire toxoplasmosis during pregnancy and fetal transmission are high because the environment is highly contaminated with oocysts. The burden of toxoplasmosis in congenitally infected children is also very high. From limited data on screening of infants forT. gondiiIgM at birth, 5–23 children are born infected per 10 000 live births in Brazil. Based on an estimate of 1 infected child per 1000 births, 2649 children with congenital toxoplasmosis are likely to be born annually in Brazil. Most of these infected children are likely to develop symptoms or signs of clinical toxoplasmosis. Among the congenitally infected children whose clinical data are described in this review, several died soon after birth, 35% had neurological disease including hydrocephalus, microcephaly and mental retardation, 80% had ocular lesions, and in one report 40% of children had hearing loss. The severity of clinical toxoplasmosis in Brazilian children may be associated with the genetic characteristics ofT. gondiiisolates prevailing in animals and humans in Brazil.

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Behaviour of indirect immunofluorescence test and some cerebrospinal parameters in neurocysticercosis

Simonetti

Teixeira²

1987

Arq. Neuro-Psiquiatr.

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Cerebrospinal fluid from 53 patients with clinical evidence of neurocysticercosis and 11 who suffered from several diseases were studied to evaluate the behaviour of indirect immunofluorescence test and some parameters of routine analysis. In neurocysticercosis there were pleocytosis in 88.7% of cases, eosinophilorrachia in 60.3%, hyperproteinorrachia in 71.7% and hypoglucorrachia in 13.2%. The indirect immunofluorescence test was positive in 79.2% of cases but false-positive results were found when the samples showed xanthochromia or erythrocyte contamination. The authors discuss their results in comparison with those in literature and conclude that the immunofluorescent test is sensitive and useful in diagnosis of neurocysticercosis, except when the interferents previously mentioned are present.

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Análise comparativa entre as sensibilidades da reação indireta de anticorpos fluorescentes e da reação de Sabin-Feldman na pesquisa de anticorpos séricos para toxoplasmose

Cited by 8 publications

References 16 publications

The IFN-³+874T/A gene polymorphism is associated with retinochoroiditis toxoplasmosis susceptibility

The IFN-³+874T/A gene polymorphism is associated with retinochoroiditis toxoplasmosis susceptibility

Toxoplasmosis in humans and animals in Brazil: high prevalence, high burden of disease, and epidemiology

Behaviour of indirect immunofluorescence test and some cerebrospinal parameters in neurocysticercosis

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