Analgesic Effects of ChondroT in Collagenase-induced Osteoarthritis Rat Model

Abstract

Objectives The purpose of this study was to find out the effects of ChondroT on arthralgia of the Collagenase-induced osteoarthritis in rats. Methods Osteoarthritis was induced into rat by injecting Collagenase in its knee joint. Rats are divided into a total of 8 groups (n=6). Normal group was not induced for osteoarthritis whereas control groups were induced for osteoarthritis by Collagenase. Positive-A (Indomethacin) was injected with Collagenase and after 8 days, 2 mg/kg of Indomethacin was medicated. Positive-B (JOINS TAB) was injected with Collagenase and after 8 days, 20 mg/kg of JOINS TAB was medicated. Experimental groups (Chondro T) at three dose levels (50, 100 and 200 mg/kg) were injected with Collagenase and after 8days they were medicated with 10 ml/kg. Indomethacin, JOINS TAB and ChondroT were medicated each substances once a day for 10 days. Thereafter, the changes in plantar withdrawal response of osteoarthritis rats by dynamic plantar aesthesiometer were observed and then RT-PCR analysis was done to investigate the expression of related proteins. Results 1. ChondroT significantly decreased withdrawal response of mechanical allodynia compared with control group in all of the experimental groups (ChondroT-A, ChondroT-B, ChondroT-C). 2. ChondroT significantly reduced Bax/Bcl-2 ratio in all of the experimental groups (ChondroT-A, ChondroT-B, ChondroT-C). 3. ChondroT significantly reduced the expression of INF-γ compared with control group in group ChondroT-B, ChondroT-C. Conclusions This results suggest that ChondroT may be meaningful for suppressing the pain of osteoarthritis. Further study is needed to conduct a rigorous clinical research. (J Korean Med Rehabil 2016;26(3):17-30)

The Inhibitory Effect of ChondroT on Indomethacin-Induced Gastric Mucosal Injury in Rats

“…그러나 COX-1, COX-2의 억제로 인하여 혈압 상승 등 의 심혈관 관련 질환 악화와 위장관의 궤양 및 출혈 등 의 부작용이 발생된다 [18][19][20] . 21,22) . 선행연구를 통하여 ChondroT 구성 약재의 유효성분을 확인하였고 항염증효능, 연골세포 보호효 과를 입증하였다 21,[23][24][25] .…”

“…고령화 추세와 더불어 관절염 및 근골격계 질환으로NSAIDs를 장기 복용하는 노인 환자가 증가하고 있으 며 NSAIDs를 장기 복용한 환자의 25%에서 위장관 궤 양이, 이들의 2-4%에서 출혈이나 천공 등의 합병증이 유발되고 있다[33][34][35][36][37] . NSAIDs 복용에 따른 부작용 때문 에 환자별로 치료적 유익성과 부작용 위험성 사이의 균 형을 맞추고자 미국심장학회와 영국 National Institute for Health and Care Excellence (NICE)에서는 가이드라인 을 통해 환자에 맞는 적절한 사용을 권고하고 있다38,39) .증하였으며 동물모델에 대한 단회 및 반복 투여 독성실험을 통해 ChondroT의 무독성량을 설정하였고, 혈소판 응집에 대한 항응고 작용을 확인하였다18,21,25,43,44) .장장애가 유발된SD계의 흰쥐에 골관절염 치료에 사용 되는 천연물 의약품인 Joins Tab 45) 과 선택적 COX-2 억 제제인 Celebrex 46) , 그리고 저용량과 고용량의 ChondroT 를 투여하여 위 산도 측정, 위 내용물 양 측정, cytokine 측정, PGE2 측정, MPO 활성도 측정, erythrocyte와 thrombocyte 측정, 위점막 및 식도점막 손상 측정 및 조 직학적 분석의 과정을 통해 ChondroT가 위장관에 미치 는 영향을 알아보고자 하였다. 실험 기간 모든 군에서 시험물질 투여에 따른 빈사 및 폐사 동물은 관찰되지 않았다.…”