Analgesic, Anti-Inflammatory, and Chondroprotective Activities ofCryptolepis buchananiExtract:In VitroandIn VivoStudies

Hanprasertpong, Nutthiya; Teekachunhatean, Supanimit; Chaiwongsa, Rujirek; Ongchai, Siriwan; Kunanusorn, Puongtip; Sangdee, Chaichan; Panthong, Ampai; Bunteang, Samreang; Nathasaen, Narong; Reutrakul, Vichai

doi:10.1155/2014/978582

Cited by 23 publications

(23 citation statements)

References 41 publications

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“…Harvested scaffolds were digested with 2U papain at 60℃ prior to evaluating the amounts of HA, uronic acid, and DNA. The monolayer culture medium and the supernatant of papain-digested scaffolds were measured for HA by using ELISA as previously described [ 15 ]. The uronic acid and DNA contents in the supernatant of papain-digested scaffolds were assessed utilizing the carbazole method and the Hoechst 33258 dye assay, respectively, as described previously [ 7 20 ].…”

Section: Methodsmentioning

confidence: 99%

TGF-β1 upregulates the expression of hyaluronan synthase 2 and hyaluronan synthesis in culture models of equine articular chondrocytes

et al. 2018

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We investigated the effect of transforming growth factor beta 1 (TGF-β1) on equine hyaluronan synthase 2 (HAS2) gene expression and hyaluronan (HA) synthesis in culture models of articular chondrocytes. Equine chondrocytes were treated with TGF-β1 at different concentrations and times in monolayer cultures. In three-dimensional cultures, chondrocyte-seeded gelatin scaffolds were cultured in chondrogenic media containing 10 ng/mL of TGF-β1. The amounts of HA in conditioned media and in scaffolds were determined by enzyme-linked immunosorbent assays. HAS2 mRNA expression was analyzed by semi-quantitative reverse transcription polymerase chain reaction. The uronic acid content and DNA content of the scaffolds were measured by using colorimetric and Hoechst 33258 assays, respectively. Cell proliferation was evaluated by using the alamarBlue assay. Scanning electron microscopy (SEM), histology, and immunohistochemistry were used for microscopic analysis of the samples. The upregulation of HAS2 mRNA levels by TGF-β1 stimulation was dose and time dependent. TGF-β1 was shown to enhance HA and uronic acid content in the scaffolds. Cell proliferation and DNA content were significantly lower in TGF-β1 treatments. SEM and histological results revealed the formation of a cartilaginous-like extracellular matrix in the TGF-β1-treated scaffolds. Together, our results suggest that TGF-β1 has a stimulatory effect on equine chondrocytes, enhancing HA synthesis and promoting cartilage matrix generation.

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Section: Methodsmentioning

confidence: 99%

TGF-β1 upregulates the expression of hyaluronan synthase 2 and hyaluronan synthesis in culture models of equine articular chondrocytes

et al. 2018

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“…and Schutt; C. buchanani (known as Thao-En-On in Thailand) is an indigenous plant which belongs to the Asclepiadaceae family [26]. It is widely used in folk medicine in Southeast Asia for treating muscle tension and arthritis [27]. The pharmacological properties of C. buchanani include antibacterial, anti-inflammatory, analgesic, chondroprotective, and hepatoprotective effects [28].…”

Section: Introductionmentioning

confidence: 99%

Exploration of the Antimicrobial and Catalytic Properties of Gold Nanoparticles Greenly Synthesized by Cryptolepis buchanani Roem. and Schult Extract

Wongyai

Wintachai

Maungchang

et al. 2020

Journal of Nanomaterials

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A green, simple, and rapid synthesis of gold nanoparticles using plant extract, Cryptolepis buchanani Roem. and Schult, and their applications are first described in this paper. The formation of gold nanoparticles was visually observed by the appearance of a ruby red color, which was further indicated by an absorption peak at 530 nm in UV-Vis spectroscopy. Optimization of reaction parameters for the gold nanoparticles was also investigated. Various analytical techniques were employed as part of the process of characterizing the resulting gold nanoparticles. Fourier transform infrared (FTIR) analysis revealed that the phenol compounds present in the extract were responsible for gold(III) reduction and stabilization of gold nanoparticles. Transmission electron microscopy (TEM) analysis showed that the gold nanoparticles were spherical in shape with an average diameter of 11 nm. Powder X-ray diffraction (XRD) pattern indicated that the green synthesis approach produced highly crystalline, face-centered cubic gold nanoparticles. Energy-dispersive X-ray spectroscopy (EDS) measurements confirmed the presence of elemental gold in the prepared nanoparticles. The negative zeta potential value of gold nanoparticles was found to be -30.28 mV. The green synthesized gold nanoparticles expressed effective antibacterial activity against Staphylococcus aureus, methicillin-resistant Staphylococcus aureus, and Acinetobacter baumannii and exhibited an excellent catalytic property in terms of its reduction ability of methylene blue.

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“…Cryptolepis sanguinolenta is a plant indigenous to Africa where it has been used in traditional medicine to treat malaria, tuberculosis, hepatitis, and septicemia (45). Cryptolepis sanguinolenta has been shown in preclinical studies to have antiinflammatory (46,47) antibacterial (48)(49)(50), anti-fungal (51), anti-amoebic (52), and anti-malarial (53,54) properties. Two preliminary clinical studies have documented significant efficacy in treating uncomplicated malaria without signs of overt toxicity (55).…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Natural and Botanical Medicines for Activity Against Growing and Non-growing Forms of B. burgdorferi

Feng

Leone

Schweig³

et al. 2020

Front. Med.

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Lyme disease is the most common vector-borne disease in the US and Europe. Although the current recommended Lyme antibiotic treatment is effective for the majority of Lyme disease patients, about 10-20% of patients continue to suffer from persisting symptoms. There have been various anecdotal reports on the use of herbal extracts for treating patients with persisting symptoms with varying degree of improvements. However, it is unclear whether the effect of the herb products is due to their direct antimicrobial activity or their effect on host immune system. In the present study, we investigated the antimicrobial effects of 12 commonly used botanical medicines and three other natural antimicrobial agents for potential anti-Borrelia burgdorferi activity in vitro. Among them, 7 natural product extracts at 1% were found to have good activity against the stationary phase B. burgdorferi culture compared to the control antibiotics doxycycline and cefuroxime. These active botanicals include Cryptolepis sanguinolenta, Juglans nigra (Black walnut), Polygonum cuspidatum (Japanese knotweed), Artemisia annua (Sweet wormwood), Uncaria tomentosa (Cat's claw), Cistus incanus, and Scutellaria baicalensis (Chinese skullcap). In contrast, Stevia rebaudiana, Andrographis paniculata, Grapefruit seed extract, colloidal silver, monolaurin, and antimicrobial peptide LL37 had little or no activity against stationary phase B. burgdorferi. The minimum inhibitory concentration (MIC) values of Artemisia annua, Juglans nigra, and Uncaria tomentosa were quite high for growing B. burgdorferi, despite their strong activity against the non-growing stationary phase B. burgdorferi. On the other hand, the top two active herbs, Cryptolepis sanguinolenta and Polygonum cuspidatum, showed strong activity against both growing B. burgdorferi (MIC = 0.03-0.06% and 0.25-0.5%, respectively) and non-growing stationary phase B. burgdorferi. In subculture studies, only 1% Cryptolepis sanguinolenta extract caused complete eradication, while doxycycline and cefuroxime and other active herbs could not eradicate B. burgdorferi stationary phase cells as many spirochetes were visible after 21-day subculture. Further studies are needed to identify the active constituents of the effective botanicals and evaluate their combinations for more effective eradication of B. burgdorferi in vitro and in vivo. The implications of these findings for improving treatment of persistent Lyme disease are discussed.

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Analgesic, Anti-Inflammatory, and Chondroprotective Activities ofCryptolepis buchananiExtract:In VitroandIn VivoStudies

Cited by 23 publications

References 41 publications

TGF-β1 upregulates the expression of hyaluronan synthase 2 and hyaluronan synthesis in culture models of equine articular chondrocytes

TGF-β1 upregulates the expression of hyaluronan synthase 2 and hyaluronan synthesis in culture models of equine articular chondrocytes

Exploration of the Antimicrobial and Catalytic Properties of Gold Nanoparticles Greenly Synthesized by Cryptolepis buchanani Roem. and Schult Extract

Evaluation of Natural and Botanical Medicines for Activity Against Growing and Non-growing Forms of B. burgdorferi

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