Analgesic activity of Alpinia galanga extract in mice models and TNF-alpha receptor computational docking analysis on its leads with pharmacokinetics prediction

Abstract

Background: Alpinia galanga is an ayurvedic herb recognized and used across many traditional medicine systems for its analgesic and anti-inflammatory activity. The present study scientifically validates the potential anti nociceptive action of ethanolic extract of Alpinia galanga by chemical, neurogenic and inflammatory nociception model in mice followed by identification of potential lead compound by computational analysis.Methods: The assessment of anti nociceptive action is evaluated by Acetic acid induced abdominal constrictions and Formalin assay on ethonolic extract of Alpinia galanga, followed by 20 compounds with known chemical structure of Alpinia galanga is subjected to computational analysis to predict possible lead compound with desirable pharmacokinetic and drug like features.Results: The percentage inhibition rate of Aspirin (100mg/kg) was 82.15% compared to Alpinia galanga (100mg/kg) 19.63%, (200mg/kg) 33.02% and (400mg/kg) 57.13% by acetic acid induced abdominal constrictions antinociceptive mice model. Alpinia galanga 400mg/kg (71.70%) had comparable percentage inhibition of nociception to standard group indomethacin (88.71%) in formalin induced nociceptive mice model. Among 20 compounds screened for pharmacokinetic and drug like features, Galanal B had the binding free energy -56.664 when compared to control compound 2AZ5-56.000.Conclusions: The Alpinia galanga extract had significant anti nociceptive activity and followed by computational analysis of 20 compounds with known chemical structure predicted Galanal B as lead compound with best insilico pharmacokinetic and drug like features.

Recent Findings on Alpinia Galanga (L.) Wild for the Treatment of Arthritis Part-1

“…The results indicated that a central mechanism of analgesic action was exhibited at 400 mg/kg and a peripheral mechanism of analgesic action was exhibited at 800 mg/kg. 25 In Swiss albino mice, Subhash et al 26 observed similar results. They further performed in silico studies using Schrodinger software.…”

Phytochemical and Pharmacological activities of Alpinia galangal: A review

“…Alpinia galanga 400mg/kg (71.70%) had comparable percentage inhibition of nociception to standard group indomethacin (88.71%) in the formalin-induced nociceptive mice model. Among 20 compounds screened for pharmacokinetic and druglike features, Galanal B had the binding free energy -56.664 when compared to control compound 2AZ5-56.000 (Subash et al, 2018). Ethanolic extract of Alpinia galanga rhizomes was given orally to experimental animals.…”

Evaluation of Antioxidant, Analgesic and Anti-Inflammatory Properties of Sedum Adenotrichum

“…The control group received 0.1 mL/10 g of the vehicle. The reaction time (sec) of each group was noted with control and demonstrated as percent inhibition (16).…”