Anaesthetic efficacy of unilamellar and multilamellar liposomal formulations of articaine in inflamed and uninflamed tissue

Silva, Camila Batista da; Groppo, Francisco Carlos; Santos, Cleiton Pita dos; Serpe, Luciano; Franz-Montan, Michelle; Paula, Eneida de; Ranali, José; Volpato, Maria Cristina

doi:10.1016/j.bjoms.2016.01.005

Cited by 11 publications

(12 citation statements)

References 23 publications

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“…The great lipid solubility of articaine in additional to its high concentration formula as a 4% solution could be the reason for increasing the number of uncharged local anesthetic molecules crossing the nerve membrane. [341718] The result of this study is in consistence with the findings of several studies[16192021] that reported the success of articaine infiltration in achieving faster onset time and more profound level of anesthesia.…”

Section: Discussionsupporting

confidence: 86%

“…This means more uncharged base local anesthetic molecules are present to diffuse through the nerve sheath and as a consequently faster onset time must be achieved. [41516] On the other hand, this study revealed that articaine has slightly faster onset time than mepivacaine. Although the tissue diffusion characteristics of articaine are not well-understood,[1] there is one possible account for the superiority of articaine over mepivacaine in terms of onset time of action.…”

Section: Discussionmentioning

confidence: 66%

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Comparison of onset anesthesia time and injection discomfort of 4% articaine and 2% mepivacaine during teeth extractions

et al. 2017

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Objective:To investigate the speed of action and injection discomfort of 4% articaine and 2% mepivacaine for upper teeth extractions.Materials and Methods:Forty-five patients were included in the articaine 4% group, and 45 in the mepivacaine 2% control group. After all injections, soft and hard tissue numbness was objectively gauged by dental probe at intervals of 15 s. Furthermore, the discomfort of the injections were recorded by the patients after each treatment on standard 100 mm visual analog scales, tagged at the endpoints with “no pain” (0 mm) and “unbearable pain” (100 mm).Results:There were significant differences in the meantime of first numbness to associated palatal mucosa and tooth of patients between mepivacaine and articaine buccal infiltration (BI) groups P = 0.01 and 0.01. Patients in the articaine group recorded earlier palatal mucosa and teeth numbness than those in the mepivacaine group. With regards to the discomfort of the needle injections, palatal injection was significantly more painful than BI (t-test: P < 0.001). Articaine buccal injection was significantly more painful than mepivacaine buccal injection (t-test: P <0.001). However, articaine palatal injection was less painful than articaine BI. Clinically, anesthesia onset time was faster in anterior upper teeth than upper middle and posterior teeth.Conclusions:BIs with 4% articaine was faster in achieving palate and teeth anesthesia than 2% mepivacaine for extraction of upper maxillary teeth. Patients in mepivacaine BI and articaine palatal injection groups reported less pain with needle injection. Failure of anesthesia was noticeable with maxillary multiple-rooted teeth.

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Section: Discussionsupporting

confidence: 86%

Section: Discussionmentioning

confidence: 66%

Comparison of onset anesthesia time and injection discomfort of 4% articaine and 2% mepivacaine during teeth extractions

et al. 2017

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“…The absence of sensitivity to the electrical stimulus in two cycles of 80 mA confirmed the beginning of the effect and pulpal anesthetic efficacy and defined the latency time measured in 2 and 5 minutes. Anesthesia failure was considered when the volunteer showed sensitivity to electrical stimulus at the tenth minute after anesthetic injection 19 .…”

Section: Methodsmentioning

confidence: 99%

“…Duration of pulpal anesthesia: this corresponded to the period between the beginning of anesthetic action and the moment the tooth returned to its basal electrical stimulation threshold. The maxillary second molars were tested every 10 minutes with the application of 80 mA stimuli until they returned to the baseline threshold value 19 .…”

Section: Methodsmentioning

confidence: 99%

Assessment of anesthetic properties and pain during needleless jet injection anesthesia: a randomized clinical trial

et al. 2019

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Pain due to administration of local anesthetics is the primary reason for patients' fear and anxiety, and various methods are used to minimize it. This study aimed to measure the degree of pain during administration of anesthesia and determine the latency time and duration of pulpal anesthesia using two anesthetic methods in the maxilla. Materials and Methods: A randomized, single-blind, split-mouth clinical trial was conducted with 41 volunteers who required class I restorations in the maxillary first molars. Local anesthesia was administered with a needleless jet injection system (experimental group) or with a carpule syringe (control) using a 30-gauge short needle. The method of anesthesia and laterality of the maxilla were randomized. A pulp electric tester measured the latency time and duration of anesthesia in the second molar. Visual analogue scale (VAS) was used to measure the degree of pain during the anesthetic method. Data were tabulated and then analyzed by a statistician. The t-test was used to analyze the differences between the groups for basal electrical stimulation. Duration of anesthesia and degree of pain were compared using the Mann-Whitney test. A 5% significance level was considered. Results: There was no statistical difference in the basal electrical stimulation threshold (mA) and degree of pain between the two methods of anesthesia (p>0.05). Latency time was 2 minutes for all subjects. The duration of pulpal anesthesia showed no statistical difference (minutes) between the two methods (p<0.001), with a longer duration for the traditional method of anesthesia (median of 40 minutes). Conclusions: The two anesthetics methods did not differ concerning the pain experienced during anesthesia. Latency lasted 2 minutes for all subjects; the traditional infiltration anesthesia resulted in a longer anesthetic duration compared with the needleless jet injection.

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“…Here, we aimed to develop a translational mouse model where the same neurophysiological techniques can be used to investigate liposomal formulations of LA in vivo . To challenge the validity of the model, we tested an unilamellar liposomal formulation, consisting of a single lipid bilayer ( Betageri and Parsons, 1992 ) which has faster release profile than multilamellar liposomes ( Silva et al, 2016 ). The liposomes were filled with the LA lidocaine which has a shorter duration of action than bupivacaine in extended-release formulations ( Huynh et al, 2015 ).…”

Section: Introductionmentioning

confidence: 99%

An in Vivo Mouse Model to Investigate the Effect of Local Anesthetic Nanomedicines on Axonal Conduction and Excitability

et al. 2018

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Peripheral nerve blocks (PNBs) using local anesthetic (LA) are superior to systemic analgesia for management of post-operative pain. An insufficiently short PNB duration following single-shot LA can be optimized by development of extended release formulations among which liposomes have been shown to be the least toxic. In vivo rodent models for PNB have focused primarily on assessing behavioral responses following LA. In a previous study in human volunteers, we found that it is feasible to monitor the effect of LA in vivo by combining conventional conduction studies with nerve excitability studies. Here, we aimed to develop a mouse model where the same neurophysiological techniques can be used to investigate liposomal formulations of LA in vivo. To challenge the validity of the model, we tested the motor PNB following an unilamellar liposomal formulation, filled with the intermediate-duration LA lidocaine. Experiments were carried out in adult transgenic mice with fluorescent axons and with fluorescent tagged liposomes to allow in vivo imaging by probe-based confocal laser endomicroscopy. Recovery of conduction following LA injection at the ankle was monitored by stimulation of the tibial nerve fibers at the sciatic notch and recording of the plantar compound motor action potential (CMAP). We detected a delayed recovery in CMAP amplitude following liposomal lidocaine, without detrimental systemic effects. Furthermore, CMAP threshold-tracking studies of the distal tibial nerve showed that the increased rheobase was associated with a sequence of excitability changes similar to those found following non-encapsulated lidocaine PNB in humans, further supporting the translational value of the model.

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Anaesthetic efficacy of unilamellar and multilamellar liposomal formulations of articaine in inflamed and uninflamed tissue

Cited by 11 publications

References 23 publications

Comparison of onset anesthesia time and injection discomfort of 4% articaine and 2% mepivacaine during teeth extractions

Comparison of onset anesthesia time and injection discomfort of 4% articaine and 2% mepivacaine during teeth extractions

Assessment of anesthetic properties and pain during needleless jet injection anesthesia: a randomized clinical trial

An in Vivo Mouse Model to Investigate the Effect of Local Anesthetic Nanomedicines on Axonal Conduction and Excitability

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