Anaeroplasma bactoclasticum extracellular amylase

Abstract

Flow chart of ultrafiltration to separate amylase by molecular weight 48 Comparison of Anaeroplasma bactoclasticum amylase activity by the disc-plate assay Comparison of the effect of pH on Anaero plasma bactoclasticum amylase in various assay buffers containing 1 mM CaClg Comparison of Anaeroplasma bactoclasticum amylase production in rumen fluid and nonrumen fluid based media Comparison of Anaeroplasma bactoclasticum amylase production in different basal media Growth study of Anaeroplasma bactoclasticum in amylase production medium Anaeroplasma bactoclasticum amylase pro duction throughout growth in amylase pro duction medium Amylase activity in the supernatant fluid after the stepwise addition of ammonium sulfate Anaeroplasma bactoclasticum amylase pro duction Elution pattern of Anaeroplasma bacto clasticum partially purified amylase from a Sephadex G-200 column Elution pattern of Anaeroplasma bacto clasticum partially purified amylase from a Biogel A 0.5 m column Elution pattern of Anaeroplasma bacto clasticum partially purified amylase from a DEAE-Sepharose CL-6B column 91 93 98 148 Amylase activity in the supernatant fluid after the stepwise addition of ammonium sulfate 151