Anaerophaga thermohalophila gen. nov., sp. nov., a moderately thermohalophilic, strictly anaerobic fermentative bacterium.

Denger, Karin; Warthmann, R.; Ludwig, Wolfgang; Schink, Bernhard

doi:10.1099/00207713-52-1-173

Cited by 73 publications

(42 citation statements)

References 30 publications

(28 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…were detected in fluids cooled at the heat exchanger from 75°C to 46°C. Physiological characteristics of detected SRB and Halanaerobiaceae correspond to the subsurface environment with respect to optimum temperature, salt concentration, and pH, and are commonly found in similar anaerobic saline environments such as sediments from salt lakes (Cayol et al 1994;Jakobsen et al 2006), geothermal groundwater (Daumas et al 1988), fracture water (Chivian et al 2008), as well as in oil fields and petroleum hydrocarbon contaminated sites (Magot et al 2000;Cayol et al 2002;Denger et al 2002;Bonch-Osmolovskaya et al 2003). Halophilic Desulfohalobium utahense are known moderate halophilic and mesophilic sulfate and thiosulfate reducers that use a broad range of electron donors including various short-chain fatty acids and primary (C 1-5 ) alcohols, amino acids, H 2 /acetate and H 2 /yeast extract (Jakobsen et al 2006).…”

Section: Discussionmentioning

confidence: 99%

“…Microorganisms and parts of the biofilm were probably detached with the fluid flow as a result of mechanical processes, leading to the observed changes within the microbial community composition. Detected Desulfotomaculum are typically endospore-forming (Campbell and Postgate 1965) and also Anaerophaga thermohalophila form spore-like structures or spheres in ageing 14 cultures (Denger et al 2002). Beside fluid temperature, substrate availability is important for stimulating dormant microorganisms.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Thermal effects on microbial composition and microbiologically induced corrosion and mineral precipitation affecting operation of a geothermal plant in a deep saline aquifer

et al. 2013

View full text Add to dashboard Cite

The microbial diversity of a deep saline aquifer used for geothermal heat storage in the North German Basin was investigated. Genetic fingerprinting analyses revealed distinct microbial communities in fluids produced from the cold and warm side of the aquifer. Direct cell counting and quantification of 16S rRNA genes and dissimilatory sulfite reductase (dsrA) genes by real-time PCR proved different population sizes in fluids, showing higher abundance of bacteria and sulfate reducing bacteria (SRB) in cold fluids compared with warm fluids. The operation-dependent temperature increase at the warm well probably enhanced organic matter availability, favoring the growth of fermentative bacteria and SRB in the topside facility after the reduction of fluid temperature. In the cold well, SRB predominated and probably accounted for corrosion damage to the submersible well pump, and iron sulfide precipitates in the near wellbore area and topside facility filters. This corresponded to lower sulfate content in fluids produced from the cold well as well as higher content of hydrogen gas that was probably released from corrosion, and maybe favored growth of hydrogenotrophic SRB. This study reflects the high influence of microbial populations for geothermal plant operation, because microbiologically induced precipitative and corrosive processes adversely affect plant reliability.2

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Thermal effects on microbial composition and microbiologically induced corrosion and mineral precipitation affecting operation of a geothermal plant in a deep saline aquifer

et al. 2013

View full text Add to dashboard Cite

show abstract

“…hydrocarbonoclasticus correspond to strains confirmed to degrade hydrocarbons under anaerobic conditions [86][87][88]. M. ruestringensis was previously found to be associated with an alkane degrading microbial community [89] and Petrotoga is an anaerobic genus indigenous to oil reservoirs [90].…”

Section: Response Of Bacterial Community To Geometrymentioning

confidence: 99%

Influence of Petroleum Deposit Geometry on Local Gradient of Electron Acceptors and Microbial Catabolic Potential

Singh

Pruden

Widdowson

2012

Environ. Sci. Technol.

View full text Add to dashboard Cite

A field survey was conducted following the Deepwater Horizon blowout and it was noted that resulting coastal petroleum deposits possessed distinct geometries, ranging from small tar balls to expansive horizontal oil sheets. A laboratory study evaluated the effect of oil deposit geometry on localized gradients of electron acceptors and microbial community composition, factors that are critical to accurately estimating biodegradation rates. One-dimensional top-flow sand columns with 12-hour simulated tidal cycles compared two contrasting geometries (isolated tar "balls" versus horizontal "sheets") relative to an oil-free control. Significant differences in the effluent dissolved oxygen and sulfate concentrations were noted among the columns, indicating presence of anaerobic zones in the oiled columns, particularly in the sheet condition. iv

show abstract

“…Phylogenetic analysis based on 16S rRNA gene sequences showed that strain HS1 T was affiliated with the family Marinilabiliaceae and shared distant relationship with the previously described genera of this family. At the time of writing the family Marinilabiliaceae contained five genera: Alkaliflexus, Anaerophaga, Marinilabilia, Mangroviflexus and Geofilum (Nakagawa & Yamasato, 1996;Denger et al, 2002;Zhilina et al, 2004;Chao et al, 2012;Miyazaki et al, 2012). So far, Anaerophaga thermohalophila is the only thermophilic species in the family Marinilabiliaceae.…”

mentioning

confidence: 99%

“…Motility was observed on YTBC solid medium. Pigments were extracted and analysed according to the method described by Denger et al (2002). For growth assays under aerobic conditions, the strain was inoculated on YTBC agar plates (no reducing reagents added) and exposed to air.…”

mentioning

confidence: 99%

Thermophagus xiamenensis gen. nov., sp. nov., a moderately thermophilic and strictly anaerobic bacterium isolated from hot spring sediment

Gao

Liu

Zhang

et al. 2013

International Journal of Systematic and Evolutionary Microbiology

View full text Add to dashboard Cite

A moderately thermophilic and strictly anaerobic bacterium, designated HS1T, was isolated from offshore hot spring sediment in Xiamen, China. Cells were Gram-negative, catalase-positive, oxidase-negative, slender and flexible rods without flagella. The strain could grow at 35–55 °C (optimum at 50 °C) and in 1–8 % NaCl (w/v; optimum 2–4 %). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain HS1T was affiliated with the family Marinilabiliaceae and shared a distant relationship with the previously described genera. The isolate was most closely related to Anaerophaga thermohalophila Fru22T with 16S rRNA gene sequence similarity of 92.4 %, followed by the other members of the family Marinilabiliaceae with 88.7–91.1 % similarity. The dominant cellular fatty acids were iso-C15 : 0 and anteiso-C15 : 0. The predominant quinone was MK-7. The major polar lipids were phosphatidylethanolamine (PE) and an unknown polar lipid. The genomic DNA G+C content was 38.7 mol%. Besides the phylogenetically distant relationship, strain HS1T was obviously distinguished from the most closely related genera in several phenotypic properties including colony colour and pigment production, optimal temperature, optimal NaCl, relation to O2, bicarbonate/carbonate requirement, catalase activity, nitrate reduction, fermentation products and cellular fatty acid profile. Based on the phenotypic and phylogenetic data, strain HS1T represents a novel species of a new genus, for which the name Thermophagus xiamenensis gen. nov., sp. nov. is proposed. The type strain of the type species is HS1T ( = DSM 19012T = CGMCCC 1.5071T).

show abstract

Anaerophaga thermohalophila gen. nov., sp. nov., a moderately thermohalophilic, strictly anaerobic fermentative bacterium.

Cited by 73 publications

References 30 publications

Thermal effects on microbial composition and microbiologically induced corrosion and mineral precipitation affecting operation of a geothermal plant in a deep saline aquifer

Thermal effects on microbial composition and microbiologically induced corrosion and mineral precipitation affecting operation of a geothermal plant in a deep saline aquifer

Influence of Petroleum Deposit Geometry on Local Gradient of Electron Acceptors and Microbial Catabolic Potential

Thermophagus xiamenensis gen. nov., sp. nov., a moderately thermophilic and strictly anaerobic bacterium isolated from hot spring sediment

Contact Info

Product

Resources

About