Anaerobic solid state fermentation of cellulosic substrates with possible application to cellulase production

Vandevoorde, L.; Verstraete, Willy

doi:10.1007/bf00253536

Cited by 6 publications

(5 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition to mixing, the operating temperature is a fundamental variable aVecting reactor performance, because of improved hydrolysis rates and methane yields due to favorable kinetics at higher temperatures [22,37]. Therefore, thermophilic AD (55°C) has been found to improve both biosolids and pathogen destruction over mesophilic AD (35-37°C) [3,36].…”

Section: Introductionmentioning

confidence: 98%

Anaerobic digestion of secondary residuals from an anaerobic bioreactor at a brewery to enhance bioenergy generation

Bocher

Agler

García

et al. 2008

J Ind Microbiol Biotechnol

View full text Add to dashboard Cite

Many beer breweries use high-rate anaerobic digestion (AD) systems to treat their soluble high-strength wastewater. Biogas from these AD systems is used to offset nonrenewable energy utilization in the brewery. With increasing nonrenewable energy costs, interest has mounted to also digest secondary residuals from the high-rate digester effluent, which consists of yeast cells, bacteria, methanogens, and small (hemi)cellulosic particles. Mesophilic (37 degrees C) and thermophilic (55 degrees C) lab-scale, low-rate continuously-stirred anaerobic digestion (CSAD) bioreactors were operated for 258 days by feeding secondary residuals at a volatile solids (VS) concentration of approximately 40 g l(-1). At a hydraulic retention time (HRT) of 15 days and a VS loading rate of 2.7 g VS l(-1) day(-1), the mesophilic bioreactor showed an average specific volumetric biogas production rate of 0.88 l CH4 l(-1) day(-1) and an effluent VS concentration of 22.2 g VS l(-1) (43.0% VS removal efficiency) while the thermophilic bioreactor displayed similar performances. The overall methane yield for both systems was 0.21 l CH4 g(-1) VS fed and 0.47-0.48 l CH4 g(-1) VS removed. A primary limitation of thermophilic digestion of this protein-rich waste is the inhibition of methanogens due to higher nondissociated (free) ammonia (NH3) concentrations under similar total ammonium (NH4+) concentrations at equilibrium. Since thermophilic AD did not result in advantageous methane production rates or yields, mesophilic AD was, therefore, superior in treating secondary residuals from high-rate AD effluent. An additional digester to convert secondary residuals to methane may increase the total biogas generation at the brewery by 8% compared to just conventional high-rate digestion of brewery wastewater alone.

show abstract

Section: Introductionmentioning

confidence: 98%

Anaerobic digestion of secondary residuals from an anaerobic bioreactor at a brewery to enhance bioenergy generation

Bocher

Agler

García

et al. 2008

J Ind Microbiol Biotechnol

View full text Add to dashboard Cite

show abstract

“…However, solid state fermentation technology relies on use of cheaper substrates for production of the cellulases [7][8][9][10], making it more cost effective. Also, the technology is promising due to the high product concentration, low cost of dewatering and the lower input of infrastructure and skill [11][12][13]. Higher yields of cellulase are obtained in solid state fermentation (SSF) compared to SmF [14] and production cost is reduced considerably [15].…”

Section: Introductionmentioning

confidence: 99%

Improved Cellulase Production by Trichoderma reesei RUT C30 under SSF Through Process Optimization

Singhania

Sukumaran

Pandey

2007

Appl Biochem Biotechnol

131

View full text Add to dashboard Cite

This work investigated the preparation of chitosan nanoparticles used as carriers for immobilized enzyme. The morphologic characterization of chitosan nanoparticles was evaluated by scanning electron microscope. The various preparation methods of chitosan nanoparticles were discussed and chosen. The effect of factors such as molecular weight of chitosan, chitosan concentration, TPP concentration, and solution pH on the size of chitosan nanoparticles was studied. Based on these results, response surface methodology was employed. The results showed that solution pH, TPP concentration, and chitosan concentration significantly affected the size of chitosan nanoparticles. The adequacy of the predictive model equation for predicting the magnitude orders of the size of chitosan nanoparticles was verified effectively by the validation data. Immobilization conditions were investigated as well. The minimum particles size was about 42 +/- 5 nm under the optimized conditions. The optimal conditions of immobilization were as follows: one milligram of neutral proteinase was immobilized on chitosan nanoparticles for about 15 min at 40 degrees C. Under the optimized conditions, the enzyme activity yield was 84.3%.

show abstract

“…The majority of SSC studies involve mesophilic and aerobic fungi. However, investigations with bacterial species are increasing for enzyme and metabolite production by SSC, including some thermophiles and anaerobes (7–9). Among these studies, only two works involved the use of thermophilic anaerobic microorganisms, and they both studied only wheat residues as the substrate (7, 9 ).…”

Section: Introductionmentioning

confidence: 99%

“…However, investigations with bacterial species are increasing for enzyme and metabolite production by SSC, including some thermophiles and anaerobes (7–9). Among these studies, only two works involved the use of thermophilic anaerobic microorganisms, and they both studied only wheat residues as the substrate (7, 9 ). The use of thermophilic anaerobic organisms in this work could obviate many of the current technical difficulties experienced in SSC of aerobic mesophilic organisms.…”

Section: Introductionmentioning

confidence: 99%

Screening of Thermophilic Anaerobic Bacteria for Solid Substrate Cultivation on Lignocellulosic Substrates

Chinn

Nokes

Strobel³

2006

Biotechnol. Prog.

View full text Add to dashboard Cite

Interest in solid substrate cultivation (SSC) techniques is gaining for biochemical production from renewable resources; however, heat and mass transfer problems may limit application of this technique. The use of anaerobic thermophiles in SSC offers a unique solution to overcoming these challenges. The production potential of nine thermophilic anaerobic bacteria was examined on corn stover, sugar cane bagasse, paper pulp sludge, and wheat bran in submerged liquid cultivation (SmC) and SSC. Production of acetate, ethanol, and lactate was measured over a 10 day period, and total product concentrations were used to compare the performance of different organism-substrate combinations using the two cultivation methods. Overall microbial activity in SmC and SSC was dependent on the organism and growth substrate. Clostridium thermocellum strains JW20, LQRI, and 27405 performed significantly better in SSC when grown on sugar cane bagasse and paper pulp sludge, producing at least 70 and 170 mM of total products, respectively. Growth of C. thermocellum strains in SSC on paper pulp sludge proved to be most favorable, generating at least twice the concentration of total products produced in SmC (p-value < 0.05). Clostridium thermolacticum TC21 demonstrated growth on all substrates producing 30-80 and 60-116 mM of total product in SmC and SSC, respectively. Bacterial species with optimal growth temperatures of 70 degrees C grew best on wheat bran in SmC, producing total product concentrations of 45-75 mM. For some of the organism-substrate combinations total end product concentrations in SSC exceeded those in SmC, indicating that SSC may be a promising alternative for microbial activity and value-added biochemical production.

show abstract

Anaerobic solid state fermentation of cellulosic substrates with possible application to cellulase production

Cited by 6 publications

References 14 publications

Anaerobic digestion of secondary residuals from an anaerobic bioreactor at a brewery to enhance bioenergy generation

Anaerobic digestion of secondary residuals from an anaerobic bioreactor at a brewery to enhance bioenergy generation

Improved Cellulase Production by Trichoderma reesei RUT C30 under SSF Through Process Optimization

Screening of Thermophilic Anaerobic Bacteria for Solid Substrate Cultivation on Lignocellulosic Substrates

Contact Info

Product

Resources

About