Anaerobic soil disinfestation reduces survival and infectivity of Phytophthora nicotianae chlamydospores in pepper

Serrano-Pérez, Paula; Rosskopf, Erin N.; Santiago, Ana de; Rodríguez‐Molina, M. C.

doi:10.1016/j.scienta.2016.12.003

Cited by 40 publications

(36 citation statements)

References 63 publications

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“…Significant effort has been targeted toward enhancing ASD efficacy for soil-borne disease control with an emphasis on optimizing implementation variables such as carbon source (Butler et al, 2012; Hewavitharana et al, 2014; Serrano-Pérez et al, 2017; Shrestha et al, 2018), irrigation volume and duration (Daugovish et al, 2015), length of the incubation period, and the effects of soil temperature (Hewavitharana et al, 2015). Concurrent studies have examined the influence of these variables on the proposed mechanisms that yield disease suppression in response to ASD.…”

Section: Introductionmentioning

confidence: 99%

Temporal Dynamics of the Soil Metabolome and Microbiome During Simulated Anaerobic Soil Disinfestation

et al. 2019

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Significant interest exists in engineering the soil microbiome to attain suppression of soil-borne plant diseases. Anaerobic soil disinfestation (ASD) has potential as a biologically regulated disease control method; however, the role of specific metabolites and microbial community dynamics contributing to ASD mediated disease control is mostly uncharacterized. Understanding the trajectory of co-evolutionary processes leading to syntrophic generation of functional metabolites during ASD is a necessary prelude to the predictive utilization of this disease management approach. Consequently, metabolic and microbial community profiling were used to generate highly dimensional datasets and network analysis to identify sequential transformations through aerobic, facultatively anaerobic, and anaerobic soil phases of the ASD process and distinct groups of metabolites and microorganisms linked with those stages. Transient alterations in abundance of specific microbial groups, not consistently accounted for in previous studies of the ASD process, were documented in this time-course study. Such events initially were associated with increases and subsequent diminution in highly labile metabolites conferred by the carbon input. Proliferation and dynamic compositional changes in the Firmicutes community continued throughout the anaerobic phase and was linked to temporal changes in metabolite abundance including accumulation of small chain organic acids, methyl sulfide compounds, hydrocarbons, and p-cresol with antimicrobial properties. Novel potential modes of disease control during ASD were identified and the importance of the amendment and “community metabolism” for temporally supplying specific classes of labile compounds were revealed.

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Section: Introductionmentioning

confidence: 99%

Temporal Dynamics of the Soil Metabolome and Microbiome During Simulated Anaerobic Soil Disinfestation

et al. 2019

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“…It has been found to be effective in suppressing numerous soil borne plant pathogens including Fusarium oxysporum f. sp. cubense (FOC) (Wen et al 2015), Rhizoctonia solani AG-5 and Pratylenchus penetrans (Hewavitharana and Mazzola 2016), Meloidogyne arenaria, M. incognita and M. javanica (Kokalis-Burelle et al 2013) Phytophthora nicotianae (Serrano-Pérez et al 2017), and Sclerotium rolfsii (Shrestha et al 2018). Interestingly it has been found that the suppression depends on the C source added.…”

Section: Introductionmentioning

confidence: 99%

Carbon source dependent-anaerobic soil disinfestation (ASD) mitigates the sclerotial germination of Sclerotinia sclerotiorum

Mahalingam

Rajapakse

Somachandra³

et al. 2020

Trop. plant pathol.

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Though Sclerotinia sclerotiorum is a well-studied plant pathogen that causes significant economic damage worldwide, sustainable and environmental friendly control methods are difficult to establish due to it wide host range, cosmopolitan distribution and production of recalcitrant structures that can survive in soil for a long time. The pathogen was found causing a severe disease incidence on cabbage in 2016 in Sri Lanka. It was hypothesized that a) isolates of the recent disease outbreak display cross resistance to commonly applied fungicides and b) carbon (C) source supplemented Anaerobic Soil Disinfestation (ASD) is effective in mitigating the germination of sclerotia. In vitro fungicide sensitivity assays showed large variation in mycelial growth inhibition indicating high adaptability of the population towards environmental fluctuations and management practices. Signatures of cross resistance were evident. ASD was carried out using cabbage (Brassica oleracea) and leek (Allium ampeloprasum) cull piles, durian (Durio zibethinus) peels and grass cuttings (Axonopus compressus) as C sources and determined the sclerotial viability. Cabbage and leek cull piles at rates of 60-100 mg/g soil completely inhibited sclerotial germination. Maintaining anaerobic condition along with C source amendments was found to be a critical step in mitigating the sclerotial germination. GC-MS analysis of the volatiles of cabbage leaves, leeks and durian further confirmed the presence of various bioactive compounds with potential antifungal activity. Therefore, in addition to elevated microbial activity in treatments, the volatiles of C sources may have helped mitigating sclerotial germination.

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“…During the treatment, anoxic conditions in the soil are maintained with rather higher temperature (around 30 °C) under the sunlight. Effectiveness of BSD treatments to kill or inactivate many kinds of soil-borne plant pathogens has been demonstrated (Browne et al 2018; Butler et al 2014; Huang et al 2016; Mazzola et al 2018; Meng et al 2018; Messiha et al 2007; Muramoto et al 2014; Serrano-Pérez et al 2017; Shennan et al 2018; Shrestha et al 2018). Mowlick et al (2012, 2013a, b, c, 2014) reported that obligate anaerobic bacteria, mainly various linages from the class Clostridia , proliferate in the soil under such treatments.…”

Section: Introductionmentioning

confidence: 99%

Production of β-1,3-glucanase and chitosanase from clostridial strains isolated from the soil subjected to biological disinfestation

et al. 2019

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Biological soil disinfestation (BSD) or anaerobic (reductive) soil disinfestation (ASD/RSD) is a bioremediation method used to eliminate soil-borne plant pathogens by exploiting the activities of anaerobic bacteria in soil. In this study, two obligate anaerobic bacterial strains isolated from BSD-treated soil and identified as Clostridium beijerinckii were examined for their abilities to suppress the spinach wilt disease pathogen ( Fusarium oxysporum f. sp. spinaciae ) as a representative soil-borne fungal plant pathogen. Both strains degraded β-1,3-glucan and chitosan, two major polysaccharide components of ascomycetes fungal cell wall, supplemented in the medium. β-1,3-Glucanase was detected in the supernatants of cultures supplemented with different types of glucan. Similarly, chitosanase was detected in cultures supplemented with chitosan. Both the enzyme activities were also detected in cultures containing glucose as a substrate. Live cells of F. oxysporum f. sp. spinaciae that were co-incubated with each anaerobic strain under anaerobic conditions using glucose as a substrate died during incubation. Freeze-dried dead fungal biomass of the pathogen, when added to the culture, supported good growth of both anaerobes and production of both enzymes. Severe and nearly complete degradation of both live and dead fungal cells during incubation with anaerobic bacteria was observed by fluorescence microscopy. When individual anaerobic bacterial strain was co-incubated with live pathogenic fungal cells in wheat bran, a popular material for BSD-treatment, both the strains grew well and killed the fungal pathogen promptly by producing both enzymes. These results indicate that both the bacterial strains attack the fungal cells by releasing extracellular fungal cell wall-degrading enzymes, thereby eliminating the pathogen.

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Anaerobic soil disinfestation reduces survival and infectivity of Phytophthora nicotianae chlamydospores in pepper

Cited by 40 publications

References 63 publications

Temporal Dynamics of the Soil Metabolome and Microbiome During Simulated Anaerobic Soil Disinfestation

Temporal Dynamics of the Soil Metabolome and Microbiome During Simulated Anaerobic Soil Disinfestation

Carbon source dependent-anaerobic soil disinfestation (ASD) mitigates the sclerotial germination of Sclerotinia sclerotiorum

Production of β-1,3-glucanase and chitosanase from clostridial strains isolated from the soil subjected to biological disinfestation

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