Anaerobic soil disinfestation (ASD): a strategy for control of soil borne diseases in strawberry production

Shennan, Carol; Muramoto, Joji; Baird, Graeme; Zavatta, M.; Toyama, Lucinda; Mazzola, Mark; Koike, S.T.

doi:10.17660/actahortic.2016.1137.16

Cited by 11 publications

(4 citation statements)

References 12 publications

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“…‘Alpowa’); and rice bran (RB; Oryza sativa L., Farm Fuel Inc. LLC, Santa Cruz, CA, USA). The ‘standard rate’ of 20 t ha −1 corresponds with the rate previously employed in a multitude of controlled environments and field studies [ 17 , 23 ]. Multiple trials employed a ‘low rate’ amendment treatment due to the previously observed amplification of F. oxysporum f. sp.…”

Section: Methodsmentioning

confidence: 99%

“…Anaerobic soil disinfestation (ASD) is a soil-borne management practice that involves three steps: (i) incorporation of a labile carbon source, (ii) inundation to fill pore-spaces in soil, and (iii) covering the soil with an oxygen impermeable film, and incubating for several weeks [ 14 ]. This technique has been examined as a potential alternative to fumigation for soil-borne disease control in numerous cropping systems [ 15 ], including strawberry [ 16 , 17 ]. Disease control attained in response to ASD is thought to be derived primarily through the production of various active chemistries, such as volatile fatty acids, methyl sulfide compounds, and hydrocarbons possessing antimicrobial activity [ 18 , 19 , 20 ].…”

Section: Introductionmentioning

confidence: 99%

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Analysis of Environmental Variables and Carbon Input on Soil Microbiome, Metabolome and Disease Control Efficacy in Strawberry Attributable to Anaerobic Soil Disinfestation

et al. 2021

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Charcoal rot and Fusarium wilt, caused by Macrophomina phaseolina and Fusarium oxysporum f. sp. fragariae, respectively, are major soil-borne diseases of strawberry that have caused significant crop losses in California. Anaerobic soil disinfestation has been studied as an industry-level option to replace soil fumigants to manage these serious diseases. Studies were conducted to discern whether Gramineae carbon input type, incubation temperature, or incubation duration influences the efficacy of this disease control tactic. In experiments conducted using ‘low rate’ amendment applications at moderate day/night temperatures (24/18 °C), and carbon inputs (orchard grass, wheat, and rice bran) induced an initial proliferation and subsequent decline in soil density of the Fusarium wilt pathogen. This trend coincided with the onset of anaerobic conditions and a corresponding generation of various anti-fungal compounds, including volatile organic acids, hydrocarbons, and sulfur compounds. Generation of these metabolites was associated with increases in populations of Clostridium spp. Overall, carbon input and incubation temperature, but not incubation duration, significantly influenced disease suppression. All Gramineae carbon inputs altered the soil microbiome and metabolome in a similar fashion, though the timing and maximum yield of specific metabolites varied with input type. Fusarium wilt and charcoal rot suppression were superior when anaerobic soil disinfestation was conducted using standard amendment rates of 20 t ha−1 at elevated temperatures combined with a 3-week incubation period. Findings indicate that anaerobic soil disinfestation can be further optimized by modulating carbon source and incubation temperature, allowing the maximum generation of antifungal toxic volatile compounds. Outcomes also indicate that carbon input and environmental variables may influence treatment efficacy in a target pathogen-dependent manner which will require pathogen-specific optimization of treatment protocols.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Analysis of Environmental Variables and Carbon Input on Soil Microbiome, Metabolome and Disease Control Efficacy in Strawberry Attributable to Anaerobic Soil Disinfestation

et al. 2021

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“…Fresh leaf weight was measured in the field with a portable scale by separating leaves from stems, and dry weight was measured after 48 h of drying and then weighed using an electronic scale. In addition, the cost of disease management was estimated for each treatment combination in terms of annual stevia production with plastic mulch and irrigation based on prices of each region as reported in previous studies [33][34][35][36][37] (Tables S2-S4).…”

Section: Effectiveness Of Integrated Pathogen Management Under Field ...mentioning

confidence: 99%

Integrated Pathogen Management in Stevia Using Anaerobic Soil Disinfestation Combined with Different Fungicide Programs in USA, Mexico, and Paraguay

et al. 2023

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Stevia is a semi-perennial crop grown to obtain the diterpene glycosides in its leaves, which are processed to manufacture non-caloric sweeteners. Anaerobic soil disinfestation (ASD) and fungicide application were evaluated for the management of stevia stem rot (SSR) and Septoria leaf spot (SLS) in lab and field experiments. In 2019 and 2021, experiments using carbon sources for ASD were carried out in microplots at NCSU (Clayton, NC, USA). In 2020/21 and 2021/22 seasons, field experiments were conducted at CSAEGRO, Mexico (MX) and CEDIT, Paraguay (PY) using a 2 × 3 factorial design with two ASD treatments and three fungicide treatments. ASD treatments included soil amended with cornmeal (MX) or wheat bran (PY) at a rate of 20.2 Mg ha−1, molasses at 10.1 Mg ha−1, and non-amended controls. Fungicide applications included chemical (azoxystrobin), organic (pyroligneous acid, PA), and a non-treated control. ASD was effective in reducing sclerotia viability of Sclerotium rolfsii in laboratory assays (p < 0.0001) and microplot trials (p < 0.0001) in NC. During field trials, the viability of sclerotia was significantly reduced (p < 0.0001) in soils amended with cornmeal + molasses or wheat bran + molasses as carbon sources for ASD. While there was no significant effectiveness of ASD in reducing SLS in 2020 and 2021 or SSR in MX 2020 field trials (p = 0.83), it did exhibit efficacy on SSR in 2021 (p < 0.001). The application of fungicides was significantly effective in reducing SSR (p = 0.01) and SLS (p = 0.001), with azoxystrobin being the most consistent and PA not being statistically different from the control or azoxystrobin. The effects of ASD on fresh yield were inconsistent, exhibiting significant effects in Mexican fields in 2020 but not in 2021. During Paraguayan field trials, ASD only significantly interacted with fungicide applications in the dry yield in 2022. In the 2020/21 MX and 2020 PY field trials, fungicides were significantly effective in enhancing dry leaf yields, with azoxystrobin showing the highest consistency among treatments and PA variable control. In conclusion, utilizing ASD alongside organic fungicides can be a valuable tool for stevia farmers when the use of chemical fungicides is limited. Further research is required to enhance consistency and reduce the costs associated with these treatments under diverse edaphoclimatic conditions.

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“…In agriculture, anaerobic soil disinfestation (ASD), a preplanting soil treatment method that creates anaerobic soil conditions, was conventionally utilized to suppress the growth of soil-borne pathogens such as Ralstonia solanacearum, Fusarium oxysporum, and Verticillium spp. (Momma, 2008;Shennan et al, 2009;Ueki et al, 2018). The determinants of ASD were mainly reported as short-chain fatty acids (SCFAs), such as acetic acid and butyric acid (BA), derived from the metabolic pathways of anaerobic bacteria such as Clostridium spp.…”

Section: Introductionmentioning

confidence: 99%

An anaerobic rhizobacterium primes rice immunity

et al. 2023

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Anaerobic soil disinfestation (ASD): a strategy for control of soil borne diseases in strawberry production

Cited by 11 publications

References 12 publications

Analysis of Environmental Variables and Carbon Input on Soil Microbiome, Metabolome and Disease Control Efficacy in Strawberry Attributable to Anaerobic Soil Disinfestation

Analysis of Environmental Variables and Carbon Input on Soil Microbiome, Metabolome and Disease Control Efficacy in Strawberry Attributable to Anaerobic Soil Disinfestation

Integrated Pathogen Management in Stevia Using Anaerobic Soil Disinfestation Combined with Different Fungicide Programs in USA, Mexico, and Paraguay

An anaerobic rhizobacterium primes rice immunity

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