Anaerobic self-assembly of a regenerable bacteria-quantum dot hybrid for solar hydrogen production

Wang, Xuemeng; Chen, Lin; He, Rui; Cui, Shuo; Li, Jie; Fu, Xian-Zhong; Wu, Qi-Zhong; Liu, Houqi; Huang, Tianyin; Li, Wen‐Wei

doi:10.1039/d2nr01777f

Cited by 20 publications

(7 citation statements)

References 46 publications

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“…[ 50 , 51 , 52 ] CdSe x S 1‐ x QDs are commonly used as an excellent model for intracellular mineralization in E. coli . [ 33 , 53 , 54 ] The HM‐expressing E. coli cells were exposed to CdCl 2 , Na 2 SeO 3, and cysteine (Cys) to intracellular biomineralization QDs ( Figure 4 A ). The CLSM imaging revealed the formation of fluorescent QDs that were spatially localized near the pole regions of HM‐expressing cells (Figure 4B ), whereas the control cells rarely supported the biogenesis of fluorescent QDs, and the fluorescence was diffuse (Figure S7 , Supporting Information).…”

Section: Resultsmentioning

confidence: 99%

Liquid–Liquid Phase Separation‐Mediated Photocatalytic Subcellular Hybrid System for Highly Efficient Hydrogen Production

Yu,

Li,

et al. 2024

Advanced Science

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Plant chloroplasts have a highly compartmentalized interior, essential for executing photocatalytic functions. However, the construction of a photocatalytic reaction compartment similar to chloroplasts in inorganic–biological hybrid systems (IBS) has not been reported. Drawing inspiration from the compartmentalized chloroplast and the phenomenon of liquid–liquid phase separation, herein, a new strategy is first developed for constructing a photocatalytic subcellular hybrid system through liquid–liquid phase separation technology in living cells. Photosensitizers and in vivo expressed hydrogenases are designed to coassemble within the cell to create subcellular compartments for synergetic photocatalysis. This compartmentalization facilitates efficient electron transfer and light energy utilization, resulting in highly effective H2 production. The subcellular compartments hybrid system (HM/IBSCS) exhibits a nearly 87‐fold increase in H2 production compared to the bare bacteria/hybrid system. Furthermore, the intracellular compartments of the photocatalytic reactor enhance the system's stability obviously, with the bacteria maintaining approximately 81% of their H2 production activity even after undergoing five cycles of photocatalytic hydrogen production. The research brings forward visionary prospects for the field of semi‐artificial photosynthesis, offering new possibilities for advancements in areas such as renewable energy, biomanufacturing, and genetic engineering.

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Section: Resultsmentioning

confidence: 99%

Liquid–Liquid Phase Separation‐Mediated Photocatalytic Subcellular Hybrid System for Highly Efficient Hydrogen Production

Yu,

Li,

et al. 2024

Advanced Science

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“…QD-microbe biohybrids have been assembled by constructed microbial cells containing intracellular or extracellular biosynthesized QDs. 99 This biosystem integrates the advantages of QDs and microbes. For instance, as QDs have been used in bioimaging and photodynamic therapy, 16,137 QD-microbe hybrids can be applied in the same scenario and microbial cells can serve as the delivery vehicles.…”

Section: Discussionmentioning

confidence: 99%

“…The oxygen level is a factor that can affect the accumulation of intracellular ROS, thereby impairing the metabolism of microbial cells and their capability to biosynthesize QDs. 99 Under anaerobic conditions, E. coli cells accumulated less ROS under exposure to Cd and Se contents and exhibited better efficiency for CdSe QD biosynthesis compared to that of aerobic culture (Fig. 4B).…”

Section: Regulation Of Quantum Dot Biosynthesismentioning

confidence: 91%

“…By incubating E. coli under anaerobic conditions instead of aerobic ones, the biosynthetic efficiency of CdSe QDs was increased by two orders of magnitude, resulting in an E. coli –QD hybrid system with a quantum efficiency of 28.7% for H 2 production under visible light. 99 The as-assembled biohybrid system was applied in the treatment of wastewater containing organic chemicals to achieve reuse of waste resources and sustainable energy production.…”

Section: Applicationmentioning

confidence: 99%

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Microbial biosynthesis of quantum dots: regulation and application

Jin

et al. 2023

Inorg. Chem. Front.

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“…Our previous study demonstrated that Se could stimulate the biodetoxification of cadmium (Cd) through upregulating the synthesis of thiol protein for enhanced Se(VI) reduction and Cd complexation, which subsequently leads to the formation of less toxic CdSe nanoparticles. − However, it remains unclear whether such synergistic biodetoxification machinery can be extended to other heavy metals beyond Cd. Copper (Cu) is also a highly toxic heavy metal widely present in soil and aquatic environments. − It can inactivate enzymes and disrupt intracellular oxidative homeostasis in living organisms through directly binding to enzymes and inducing generation of reactive oxygen species. − Similar to Cd, Cu also usually co-exists with Se in diverse environments and is prone to bioaccumulation.…”

Section: Introductionmentioning

confidence: 99%

AQDS Activates Extracellular Synergistic Biodetoxification of Copper and Selenite via Altering the Coordination Environment of Outer-Membrane Proteins

Wang

Chen

et al. 2022

Environ. Sci. Technol.

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The biotransformation of heavy metals in the environment is usually affected by co-existing pollutants like selenium (Se), which may lower the ecotoxicity of heavy metals, but the underlying mechanisms remain unclear. Here, we shed light on the pathways of copper (Cu 2+ ) and selenite (SeO 3 2− ) synergistic biodetoxification by Shewanella oneidensis MR-1 and illustrate how such processes are affected by anthraquinone-2,6disulfonate (AQDS), an analogue of humic substances. We observed the formation of copper selenide nanoparticles (Cu 2−x Se) from synergistic detoxification of Cu 2+ and SeO 3 2− in the periplasm. Interestingly, adding AQDS triggered a fundamental transition from periplasmic to extracellular reaction, enabling 14.7-fold faster Cu 2+ biodetoxification (via mediated electron transfer) and 11.4-fold faster SeO 3 2− detoxification (via direct electron transfer). This is mainly attributed to the slightly raised redox potential of the heme center of AQDS-coordinated outer-membrane proteins that accelerates electron efflux from the cells. Our work offers a fundamental understanding of the synergistic detoxification of heavy metals and Se in a complicated environmental matrix and unveils an unexpected role of AQDS beyond electron mediation, which may guide the development of more efficient environmental remediation and resource recovery biotechnologies.

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Anaerobic self-assembly of a regenerable bacteria-quantum dot hybrid for solar hydrogen production

Abstract: Inorganic-biological hybrid systems (bio-hybrid), comprising fermentative bacteria and inorganic semiconductor photosensitizers for synergistic utilization of solar energy and organic wastes, offer opportunities for sustainable fuel biosynthesis, but the low quantum...

Cited by 20 publications

References 46 publications

Liquid–Liquid Phase Separation‐Mediated Photocatalytic Subcellular Hybrid System for Highly Efficient Hydrogen Production

Liquid–Liquid Phase Separation‐Mediated Photocatalytic Subcellular Hybrid System for Highly Efficient Hydrogen Production

Microbial biosynthesis of quantum dots: regulation and application

AQDS Activates Extracellular Synergistic Biodetoxification of Copper and Selenite via Altering the Coordination Environment of Outer-Membrane Proteins

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