Anaerobic Protein Purification and Kinetic Analysis via Oxygen Electrode for Studying DesB Dioxygenase Activity and Inhibition

Uchendu, Stacy; Rafalowski, Angelika; Cohn, Erin Frances; Davoren, Luke W; Taylor, Erika A.

doi:10.3791/58307

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Holo‐metalloprotein Purification1

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2021

2024

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“…Redox‐active metalloproteins with low oxidation states are often oxygen sensitive; they will oxidize upon exposure to atmospheric oxygen and must be kept in an oxygen‐free environment. Some proteins, such as those with nonheme Fe(II) cofactors, are expressed with oxygen‐sensitive metals and need to be purified under an anaerobic or hypoxic atmosphere, which can be accomplished using a polyvinyl glove bag (Uchendu, Rafalowski, Cohn, Davoren, & Taylor, 2018). It is sometimes necessary to work with purified protein samples in an anaerobic environment.…”

Section: Holo‐metalloprotein Purificationmentioning

confidence: 99%

Overview of Methods for Purification and Characterization of Metalloproteins

2021

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Metalloproteins make up one third of all proteins and perform some of the most essential reactions on earth. The unique properties of the metal ions within these proteins, and in particular of redox‐active metal ions, enables the use of a number of characterization techniques. It also necessitates unique considerations in terms of purification and characterization. In this overview, we describe the considerations and methods used for metalloprotein purification and characterization. © 2021 Wiley Periodicals LLC.

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Section: Holo‐metalloprotein Purificationmentioning

confidence: 99%