Anaerobic oxidations of cysteate: degradation via L-cysteate: 2-oxoglutarate aminotransferase in Paracoccus pantotrophus

Mikosch, Carola A. R. M.; Denger, Karin; Schäfer, Eva-Maria; Cook, Alasdair M.

doi:10.1099/13500872-145-5-1153

“…In aerobic organisms (and in anaerobic oxidations of sulphonates [29,31]), it is the acetate that is largely involved in energy conservation, the sulphite playing a minor role, being oxidized to sulphate (e.g. [6,29]).…”

Section: Discussionmentioning

confidence: 99%

Sulphoacetaldehyde sulpho-lyase (EC 4.4.1.12) from Desulfonispora thiosulfatigenes: purification, properties and primary sequence

Denger

¹

,

Ruff

²

,

Rein

³

et al. 2001

Biochemical Journal

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The strictly anaerobic bacterium Desulfonispora thiosulfatigenes ferments taurine via sulphoacetaldehyde, which is hydrolysed to acetate and sulphite by sulphoacetaldehyde sulpho-lyase (EC 4.4.1.12). The lyase was expressed at high levels and a two-step, 4.5-fold purification yielded an apparently homogeneous soluble protein, which was presumably a homodimer in its native form; the molecular mass of the subunit was about 61kDa (by SDS/PAGE). The mass was determined to be 63.8kDa by matrix-assisted laser-desorption ionization–time-of-flight (MALDI–TOF) MS. The purified enzyme converted 1mol of sulphoacetaldehyde to 1mol each of sulphite and acetate, but no requirement for thiamine pyrophosphate (TPP) was detected. The N-terminal and two internal amino acid sequences were determined, which allowed us to generate PCR primers. The gene was amplified and sequenced. The DNA sequence had no significant homologue in the databases searched, whereas the derived amino acid sequence indicated an oxo-acid lyase, revealed a TPP-binding site and gave a derived molecular mass of 63.8kDa.

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“…Sulfonates are also used as electron donors by an Alcaligenes strain [205] and a Paracoccus denitri¢cans isolate [320], in the presence of nitrate as electron acceptor. In Alcaligenes sp.…”

Section: Organosulfur Utilization Without Oxygenmentioning

confidence: 99%

Riding the sulfur cycle – metabolism of sulfonates and sulfate esters in Gram-negative bacteria

Kertesz

¹

2000

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Sulfonates and sulfate esters are widespread in nature, and make up over 95% of the sulfur content of most aerobic soils. Many microorganisms can use sulfonates and sulfate esters as a source of sulfur for growth, even when they are unable to metabolize the carbon skeleton of the compounds. In these organisms, expression of sulfatases and sulfonatases is repressed in the presence of sulfate, in a process mediated by the LysR-type regulator protein CysB, and the corresponding genes therefore constitute an extension of the cys regulon. Additional regulator proteins required for sulfonate desulfonation have been identified in Escherichia coli (the Cbl protein) and Pseudomonas putida (the AsfR protein). Desulfonation of aromatic and aliphatic sulfonates as sulfur sources by aerobic bacteria is oxygendependent, carried out by the K-ketoglutarate-dependent taurine dioxygenase, or by one of several FMNH 2 -dependent monooxygenases. Desulfurization of condensed thiophenes is also FMNH 2 -dependent, both in the rhodococci and in two Gram-negative species. Bacterial utilization of aromatic sulfate esters is catalyzed by arylsulfatases, most of which are related to human lysosomal sulfatases and contain an active-site formylglycine group that is generated post-translationally. Sulfate-regulated alkylsulfatases, by contrast, are less well characterized. Our increasing knowledge of the sulfur-regulated metabolism of organosulfur compounds suggests applications in practical fields such as biodesulfurization, bioremediation, and optimization of crop sulfur nutrition. ß

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“…Sulfonates are also used as electron donors by an Alcaligenes strain [205] and a Paracoccus denitrificans isolate [320], in the presence of nitrate as electron acceptor. In Alcaligenes sp.…”

Section: Organosulfur Utilization Without Oxygenmentioning

confidence: 99%

Riding the sulfur cycle â metabolism of sulfonates and sulfate esters in Gram-negative bacteria

Kertesz

¹

2000

FEMS Microbiology Reviews

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Sulfonates and sulfate esters are widespread in nature, and make up over 95% of the sulfur content of most aerobic soils. Many microorganisms can use sulfonates and sulfate esters as a source of sulfur for growth, even when they are unable to metabolize the carbon skeleton of the compounds. In these organisms, expression of sulfatases and sulfonatases is repressed in the presence of sulfate, in a process mediated by the LysR-type regulator protein CysB, and the corresponding genes therefore constitute an extension of the cys regulon. Additional regulator proteins required for sulfonate desulfonation have been identified in Escherichia coli (the Cbl protein) and Pseudomonas putida (the AsfR protein). Desulfonation of aromatic and aliphatic sulfonates as sulfur sources by aerobic bacteria is oxygendependent, carried out by the K-ketoglutarate-dependent taurine dioxygenase, or by one of several FMNH 2 -dependent monooxygenases. Desulfurization of condensed thiophenes is also FMNH 2 -dependent, both in the rhodococci and in two Gram-negative species. Bacterial utilization of aromatic sulfate esters is catalyzed by arylsulfatases, most of which are related to human lysosomal sulfatases and contain an active-site formylglycine group that is generated post-translationally. Sulfate-regulated alkylsulfatases, by contrast, are less well characterized. Our increasing knowledge of the sulfur-regulated metabolism of organosulfur compounds suggests applications in practical fields such as biodesulfurization, bioremediation, and optimization of crop sulfur nutrition. ß

show abstract

Anaerobic oxidations of cysteate: degradation via L-cysteate: 2-oxoglutarate aminotransferase in Paracoccus pantotrophus

Cited by 22 publications

References 44 publications

Sulphoacetaldehyde sulpho-lyase (EC 4.4.1.12) from Desulfonispora thiosulfatigenes: purification, properties and primary sequence

Sulphoacetaldehyde sulpho-lyase (EC 4.4.1.12) from Desulfonispora thiosulfatigenes: purification, properties and primary sequence

Riding the sulfur cycle – metabolism of sulfonates and sulfate esters in Gram-negative bacteria

Riding the sulfur cycle â metabolism of sulfonates and sulfate esters in Gram-negative bacteria

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Anaerobic oxidations of cysteate: degradation via L-cysteate: 2-oxoglutarate aminotransferase in Paracoccus pantotrophus

Cited by 22 publications

References 44 publications

Sulphoacetaldehyde sulpho-lyase (EC 4.4.1.12) from Desulfonispora thiosulfatigenes: purification, properties and primary sequence

Sulphoacetaldehyde sulpho-lyase (EC 4.4.1.12) from Desulfonispora thiosulfatigenes: purification, properties and primary sequence

Riding the sulfur cycle – metabolism of sulfonates and sulfate esters in Gram-negative bacteria

Riding the sulfur cycle â metabolism of sulfonates and sulfate esters in Gram-negative bacteria

Contact Info

Product

Resources

About

Riding the sulfur cycle â metabolism of sulfonates and sulfate esters in Gram-negative bacteria