Anaerobic oxidation of hydrocarbons in crude oil by new types of sulphate-reducing bacteria

Rueter, P.; Rabus, Ralf; Wilkes, Heinz; Aeckersberg, Frank; Rainey, Frederick A.; Hw, Jannasch; Widdel, Friedrich

doi:10.1038/372455a0

Cited by 443 publications

(272 citation statements)

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“…The high microbial sulfide production in these sediments fosters patchy mats of sulfide-oxidizing bacteria such as Beggiatoa (Jannasch et al, 1989). From such areas, a variety of sulfate-reducing bacteria (Rueter et al, 1994;Jeanthon et al, 2002), sulfate-reducing archaea (Burggraf et al, 1990;Khelifi et al, 2010) and methanogenic archaea (Kurr et al, 1991) have been isolated.…”

Section: Introductionmentioning

confidence: 99%

Thermophilic anaerobic oxidation of methane by marine microbial consortia

et al. 2011

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The anaerobic oxidation of methane (AOM) with sulfate controls the emission of the greenhouse gas methane from the ocean floor. AOM is performed by microbial consortia of archaea (ANME) associated with partners related to sulfate-reducing bacteria. In vitro enrichments of AOM were so far only successful at temperatures p25 1C; however, energy gain for growth by AOM with sulfate is in principle also possible at higher temperatures. Sequences of 16S rRNA genes and core lipids characteristic for ANME as well as hints of in situ AOM activity were indeed reported for geothermally heated marine environments, yet no direct evidence for thermophilic growth of marine ANME consortia was obtained to date. To study possible thermophilic AOM, we investigated hydrothermally influenced sediment from the Guaymas Basin. In vitro incubations showed activity of sulfate-dependent methane oxidation between 5 and 70 1C with an apparent optimum between 45 and 60 1C. AOM was absent at temperatures X75 1C. Long-term enrichment of AOM was fastest at 50 1C, yielding a 13-fold increase of methane-dependent sulfate reduction within 250 days, equivalent to an apparent doubling time of 68 days. The enrichments were dominated by novel ANME-1 consortia, mostly associated with bacterial partners of the deltaproteobacterial HotSeep-1 cluster, a deeply branching phylogenetic group previously found in a butane-amended 60 1C-enrichment culture of Guaymas sediments. The closest relatives (Desulfurella spp.; Hippea maritima) are moderately thermophilic sulfur reducers. Results indicate that AOM and ANME archaea could be of biogeochemical relevance not only in cold to moderate but also in hot marine habitats.

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Section: Introductionmentioning

confidence: 99%

Thermophilic anaerobic oxidation of methane by marine microbial consortia

et al. 2011

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“…Microbial studies of subsurface ecosystems, such as oilfields, have shown the presence of fermentative bacteria (Bernard et al, 1992;Bhupathiraju et al, 1991 ;Bhupathiraju & McInerney, 1993;DavydovaCharakhch'yan et al, 1993b;Fardeau et al, 1996;Holloway et al, 1980;Jeanthon et al, 1995;L'Haridon et al, 1995;Ravot et al, 1995;Stetter et al, 1993), sulfate reducers (Bhupathiraju & McInerney, 1993 ;Cord-Ruwisch et al, 1987 ;Nazina & Rozanova, 1978;Nilsen et al, 1996;Rees et al, 1995;Rozanova & Nazina, 1979;Rozanova & Galushko, 1990;Rozanova et al, 1989;Rueter et al, 1994;Stetter et al, 1993;Tardy-Jacquenod et al, 1996;Voordouw et al, 1996), and acetogens (Davydova-Charakhch'yan et al, 1993b). It has been established that methanogens…”

Section: Introductionmentioning

confidence: 99%

Methanocalculus halotolerans gen. nov., sp. nov., isolated from an oil-producing well

Ollivier

Fardeau

Cayol

et al. 1998

International Journal of Systematic Bacteriology

128

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Two irregular coccoid methanogens designated SEBR 4845T and FRlT were isolated from an oilfield in Alsace, France. Strain SEBR 4845l (T = type strain) is a hydrogenotrophic halotolerant methanogen, which grows optimally at 5 O/ O NaCl (whr) and tolerates up t o 12O/0 NaCI. It does not use methylated compounds and therefore cannot be ascribed to any of the known genera of the halophilic methylotrophic methanogens. It differs from hydrogenotrophic members of the orders Methanococcales and Methanomicrobiales in the NaCl growth range (0-12% NaCI), which is the widest reported t o date for any hydrogenotrophic methanogen. 165 rRNA gene sequence analysis indicated that strain SEBR 4845l is a novel isolate for which a new genus is proposed, Methanocalculus halotolerans gen. nov., sp. nov. (= OCM 4703 that might be indigenous to the oilfield ecosystem. Strain FRlT (= OCM 471) is a moderately halophilic methanogen which grows optimally at 10% NaCl and tolerates up t o 20% NaCI. It grows on trimethylamine and methanol as carbon and energy sources. The G+C content of its DNA is 43 mol%. It is therefore phenotypically and genotypically related t o members of the genus Methanohalophilus. This report provides evidence that methylotrophic and hydrogenotrophic, but not aceticlastic methanogens are present in a saline subsurface oilfield environment, as already observed in surface saline t o hypersaline environments.

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“…In the presence of oxygen, mono-and dioxygenases catalyze the oxidative functionalization of recalcitrant compounds, i.e., hydrocarbons. Microbial mineralization of such substances also occurs in nature in the absence of molecular oxygen, and in the last decade anaerobic bacteria have been isolated on substances such as alkylbenzenes (6,18,22), alkanes (1,7,25,26), and alkenes (15). Thus far, the biochemistry of the initial activation reactions has been revealed only for toluene.…”

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Geranic Acid Formation, an Initial Reaction of Anaerobic Monoterpene Metabolism in Denitrifying Alcaligenes defragrans

Heyen

Harder

2000

Appl Environ Microbiol

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Monoterpenes with an unsaturated hydrocarbon structure are mineralized anaerobically by the denitrifying ␤-proteobacterium Alcaligenes defragrans. Organic acids occurring in cells of A. defragrans and culture medium were characterized to identify potential products of the monoterpene activation reaction. Geranic acid (E,E-3,7-dimethyl-2,6-octadienoic acid) accumulated to 0.5 mM in cells grown on ␣-phellandrene under nitrate limitation. Cell suspensions of A. defragrans 65Phen synthesized geranic acid in the presence of ␤-myrcene, ␣-phellandrene, limonene, or ␣-pinene. Myrcene yielded the highest transformation rates. The alicyclic acid was consumed by cell suspensions during carbon limitation. Heat-labile substances present in cytosolic extracts catalyzed the formation of geranic acid from myrcene. These results indicated that a novel monoterpene degradation pathway must be present in A. defragrans.The mineralization of organic matter by microorganisms is often severely hampered by the chemical structure of the substrate. In the presence of oxygen, mono-and dioxygenases catalyze the oxidative functionalization of recalcitrant compounds, i.e., hydrocarbons. Microbial mineralization of such substances also occurs in nature in the absence of molecular oxygen, and in the last decade anaerobic bacteria have been isolated on substances such as alkylbenzenes (6, 18, 22), alkanes (1, 7, 25, 26), and alkenes (15). Thus far, the biochemistry of the initial activation reactions has been revealed only for toluene. Catalysis via radicals seems to be involved in the addition of toluene to fumarate by the enzyme benzylsuccinate synthase, a putative glycine radical enzyme (3,21). This novel enzyme raises the question of how the activation of alkanes and alkenes commences.Monoterpenes are ubiquitous alkenes in nature (Fig. 1). The physiological trait of anaerobic mineralization of monoterpenes to carbon dioxide has been demonstrated by the enrichment and isolation of denitrifying strains of Alcaligenes defragrans and Thauera terpenica (11,12,16). During growth of bicyclic monoterpenes, e.g., ␣-pinene, traces of monocyclic monoterpenes are detected, suggesting menthadienes as intermediates (19). A. defragrans cometabolically catalyzes the transformation of isolimonene to isoterpinolene. This 3,1-hydrogen-⌬ 1 -⌬ 3 -mutase reaction confirms the microbial activation of alkene bonds that are not polarized by adjacent functional groups. It also indicates the necessity for a sp 2 hybridization of the C-1 atom in order for microbial monoterpene oxidation to occur (19).It seems likely that the unsaturated monoterpenes may be transformed initially into an organic acid, because intermediates in catabolic pathways are usually organic acids, e.g., in the citrate cycle. If this acid cannot diffuse through membranes, the carbon source is fixed in the cell. To explore this hypothesis, we characterized the organic acids in cells and culture medium and studied the formation of geranic acid (3,7-dimethyl-2,6-trans,trans-octadienoic acid) in cells ...

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Anaerobic oxidation of hydrocarbons in crude oil by new types of sulphate-reducing bacteria

Cited by 443 publications

References 20 publications

Thermophilic anaerobic oxidation of methane by marine microbial consortia

Thermophilic anaerobic oxidation of methane by marine microbial consortia

Methanocalculus halotolerans gen. nov., sp. nov., isolated from an oil-producing well

Geranic Acid Formation, an Initial Reaction of Anaerobic Monoterpene Metabolism in Denitrifying Alcaligenes defragrans

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