Anaerobic metabolism of the ribbed mussel, Geukensia demissa

Ho, Ming-Shan; Zubkoff, Paul L.

doi:10.1016/0305-0491(82)90339-x

Cited by 12 publications

(10 citation statements)

References 13 publications

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“…Data in the present study allow us to make a rough calculation of metabolic arrest for M. galloprovincialis provided that two assumptions are made. We assume that the anaerobic ATP and phosphoarginine contributions to ATP provision that are established for the adductor muscle can also be applied to the whole body (perhaps a slight overestimate) and that negligible conversion of succinate to proprionate occurred during the 12 h incubation [proprionate production occurs only after a lag period; for M. edulis this is about 10 h at 20°C (Kluytmans et al 1978, Ho andZubkoff 1982)]. From the data in Fig.…”

Section: Discussionmentioning

confidence: 97%

Differential sensitivities to hypoxia by two anoxia-tolerant marine molluscs: A biochemical analysis

et al. 1991

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Abstract.The metabolic responses to a series of low oxygen tensions were compared for two species of Mediterranaean bivalves, Mytilus galloprovincialis and Scapharca inaequivalvis. Whereas both species have welldeveloped and similar tolerances of anoxia, the metabolic responses of S. inaequivalvis to low oxygen tensions indicate a substantially greater tolerance of hypoxia. Compared with M. galloprovincialis, the responses of S. inaequivalvis included the ability to maintain a constant oxygen consumption down to a much lower pO2 value (ca. 1.7 vs 3.4 ppm), and a lower critical pO2 for the recruitment of fermentative pathways of ATP production (ca. 1 vs 3 ppm). Furthermore, a graded increase in the output of anaerobic products (succinate, alanine) occured at oxygen tensions below 3 ppm in M. galloprovincialis and reached a maximum at 1.6 ppm whereas in S. inaequivalvis the net accumulation of anaerobic products at the lowest oxygen tension tested (0.5 ppm) was still substantially less than the level of production output in complete anoxia. This suggests that fermentative pathways are maximally activated at all oxygen tensions below 1.6 ppm in M. galloprovincialis whereas rates of anaerobic pathways are still less than maximum at 0.5 ppm in S. inaequivalvis. These results indicate that in situations of declining oxygen tensions, such as occur due to eutrophication, M. galloprovincialis would not only begin to experience metabolic stress at higher oxygen tensions than S. inaequivalvis but would experience greater stress at any given pO2. Such differences in hypoxia tolerances may explain the success of the recently introduced S. inaequivalvis in out-competing the native M. galloprovincialis in the Adriatic Sea.

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Section: Discussionmentioning

confidence: 97%

Differential sensitivities to hypoxia by two anoxia-tolerant marine molluscs: A biochemical analysis

et al. 1991

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“…One of the hallmarks of bivalve anaerobic metabolism is the use of fermentative pathways that produce succinate and propionate as end products, rather than lactate as is more commonly found in vertebrates (Hochachka and Mustafa, 1972;Kluytmans et al, 1977;Ho and Zubkoff, 1982). Succinate/propionate fermentation appears to have evolved in eukaryotes such as helminth parasites (Barrett, 1991;Müller et al, 2012) and bivalves that undergo…”

Section: Discussion Anaerobic Metabolismmentioning

confidence: 99%

“…The Journal of Experimental Biology (2014) organism (De Zwaan and Zandee, 1972;De Zwaan and van Marrewijk, 1973;Kluytmans et al, 1977;Ho and Zubkoff, 1982). The alternative aerobic and anaerobic pathways used by Mytilid mussels are shown in Fig.…”

Section: Research Articlementioning

confidence: 99%

Changes in protein expression in the salt marsh musselGeukensia demissa: evidence for a shift from anaerobic to aerobic metabolism during prolonged aerial exposure

Fields

Eurich

Gao

et al. 2014

Journal of Experimental Biology

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During aerial exposure (emersion), most sessile intertidal invertebrates experience cellular stress caused by hypoxia, and the amount and types of hypoxia-induced stress will differ as exposure time increases, likely leading to altered metabolic responses. We examined proteomic responses to increasing emersion times and decreasing recovery (immersion) times in the mussel Geukensia demissa, which occurs in salt marshes along the east coast of North America. Individuals are found above mean tide level, and can be emersed for over 18 h during spring tides. We acclimated mussels to full immersion at 15°C for 4 weeks, and compared changes in gill protein expression between groups of mussels that were continually immersed (control), were emersed for 6 h and immersed during recovery for 18 h (6E/18R), were emersed for 12 h and recovered for 12 h (12E/12R), or were emersed for 18 h with a 6 h recovery (18E/6R). We found clear differences in protein expression patterns among the treatments. Proteins associated with anaerobic fermentation increased in abundance in 6E/18R but not in 12E/12R or 18E/6R. Increases in oxidative stress proteins were most apparent in 12E/12R, and in 18E/6R changes in cytoskeletal protein expression predominated. We conclude that G. demissa alters its strategy for coping with emersion stress over time, relying on anaerobic metabolism for short-to medium-duration exposure, but switching to an air-gaping strategy for long-term exposure, which reduces hypoxia stress but may cause structural damage to gill tissue.

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“…MDH supports anaerobic metabolism in bivalves by reoxidizing NADH produced by glyceraldehyde 3-phosphate dehydrogenase and facilitating carbon transport via the malate/ aspartate shuttle to the mitochondria (Ho and Zubkoff 1982;Gade 1983;de Zwaan and Dando 1984). In the mitochondrion, conversion of oxaloacetate to succinate and propionate yields more ATP per unit carbon than classical fermentation pathways (reviewed by Livingstone 1982;Schulz et al 1982;de Zwaan 1983;de Zwaan and Dando 1984;Larade and Storey 2002).…”

Section: Introductionmentioning

confidence: 98%

Interindividual variation of malate dehydrogenase activity in the oyster Crassostrea virginica

Ulrich

Marsh

2006

Marine and Freshwater Behaviour and Physiology

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Intertidal environments are dynamic, stressful niches and variation in physiological parameters may determine distribution and survival of individuals in a population. We demonstrated that mitochondria of the oyster Crassostrea virginica oxidize malate more readily than other Krebs cycle intermediates and investigated the level of interindividual variability in oyster malate dehydrogenase (MDH) activity and total protein content in muscle tissues. Both MDH activity and total protein evidenced a high level of interindividual variation in heart and adductor among a sample of more than 50 oysters. Normalization to total DNA failed to explain the variation in either MDH activity or protein content of phasic adductor and explained less than 40% of the variation in heart. This range of MDH titers defines a continuum of biochemical phenotypes important for understanding the relative selection forces operative on metabolic pathways within the muscles of the oyster.

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Anaerobic metabolism of the ribbed mussel, Geukensia demissa

Cited by 12 publications

References 13 publications

Differential sensitivities to hypoxia by two anoxia-tolerant marine molluscs: A biochemical analysis

Differential sensitivities to hypoxia by two anoxia-tolerant marine molluscs: A biochemical analysis

Changes in protein expression in the salt marsh musselGeukensia demissa: evidence for a shift from anaerobic to aerobic metabolism during prolonged aerial exposure

Interindividual variation of malate dehydrogenase activity in the oyster Crassostrea virginica

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