Anaerobic Metabolism of Biodiesel and Its Impact on Metal Corrosion

Aktas, Deniz F.; Lee, Jason S.; Little, Brenda J.; Ray, Richard I.; Davidova, Irene A.; Lyles, Christopher N.; Suflita, Joseph M.

doi:10.1021/ef100084j

Cited by 89 publications

(73 citation statements)

References 33 publications

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“…The more extensive formation of primary oxidation products such as hydroperoxides and conjugated dienes then continuously occurs from the decomposition of unsaturated fatty acids [19], leading to the rapid degeneration of fuel properties such as the flash point, cetane index, and heating value. The presence of water in liquid fuel may also induce the formation of microorganisms and speed up the corrosion of metallic engine parts [20]. Hence, reducing the water content in liquid fuel to as low a level as possible is preferable to retain stable and superior fuel characteristics.…”

Section: Water Contentmentioning

confidence: 99%

Fuel Characteristics of Biodiesel Produced from a High-Acid Oil from Soybean Soapstock by Supercritical-Methanol Transesterification

Lin

2012

Energies

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A supercritical methanol transesterification method was applied to produce biodiesel from the high-acid oil of soybean soapstock. The fuel properties of biodiesel produced with various molar ratios of methanol to raw oil were analyzed and compared in this experimental study. Oleic acid (C18:1), linoleic acid (C18:2), and palmitic acid (C16:0)were the three main compounds in the high-acid oil-biodiesel. The saturated fatty acid content of the high-acid oil increased significantly due to the supercritical-methanol transesterification reaction. The fuel characteristics of the resulting high-acid oil, including the specific gravity and kinematic viscosity, were also greatly improved. The saturated fatty acid content of the biodiesel produced from the high-acid oil was higher than that of biodiesel from waste cooking oil produced by the subcritical transesterification using a strongly alkaline catalyst. The high-acid oil-biodiesel that was produced with a molar ratio of methanol to raw oil of 42 had the best fuel properties, including a higher distillation temperature and cetane index and a lower kinematic viscosity and water content, among the biodiesels with different molar ratios.

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Section: Water Contentmentioning

confidence: 99%

Fuel Characteristics of Biodiesel Produced from a High-Acid Oil from Soybean Soapstock by Supercritical-Methanol Transesterification

Lin

2012

Energies

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“…However, depending on the characteristics of the site and the amount spilled, anaerobic conditions may prevail over the aerobic ones. Al-though this is the case, few studies exist on the anaerobic biodegradation of biodiesel (Aktas et al 2010;Corseuil et al 2011b;Lapinskienne and Martinkus 2007;Ramos et al 2013;Sorensen et al 2011).…”

Section: Introductionmentioning

confidence: 99%

“…On the few existing studies on anaerobic conditions (Aktas et al 2010;Corseuil et al 2011b;Lapinskienne and Martinkus 2007;Ramos et al 2013;Sorensen et al 2011), some focused on evaluating the degradation in terms of LCFA (long-chain fatty acids), nitrates, and sulfates (Corseuil et al 2011b;Liu and Suflita 1994), and also on biodiesel impacts, for example, on metal corrosion (Aktas et al 2010). One recent study examined the efficacy of ammonium acetate for the biostimulation of anaerobic BTEX removal in the field at a B20 contaminated site (Ramos et al 2013).…”

Section: Introductionmentioning

confidence: 99%

Influence of the Anaerobic Biodegradation of Different Types of Biodiesel on the Natural Attenuation of Benzene

Borges¹,

Dias²,

Danko³

2014

Water Air Soil Pollut

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In the present research work, different types of biodiesel were produced by a homogeneous alkali transesterification reaction using soybean oil, pork lard, and castor bean oil as raw materials, to evaluate how their different compositions may affect the biodegradability, namely, in the presence of benzene. Biodiesel was characterized according to the European standard EN 14214. The anaerobic biodegradation of the different types of biodiesel was examined as well as its influence on the biodegradation of benzene. Analyses were performed to determine the volume of methane (directly related to the anaerobic biodegradation of bio-diesel), the concentration of benzene over time, and the production of organic acids. The results showed methane production resulting from the anaerobic degradation of all biodiesel types. The differences between the degradation behavior of each fuel were negligible, contrary to what was expected; however, the amount of methane produced was low due to nutrient limitations. This fact was confirmed by the organic acid analysis as well as by the addition of new media. Anaerobic benzene biodegradation was found to be negatively impacted by the presence of all biodiesel types on average; therefore, the results of this study may impact management of sites that contain biodiesel and fuel hydrocarbon contamination.

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“…The entire content of the bottles was extracted with ethyl acetate (10% [vol/vol]; 4 times). The extracts were dried over anhydrous Na 2 SO 4 , concentrated by rotary evaporation, and reduced further under a stream of N 2 to a volume of 50 l. The extracts and the phenol-D 6 standard were derivatized with N,O-bis(trimethylsilyl)trifluoroacetamide (BSTFA) (Pierce Chemical Co., Rockford, IL) prior to the analyses of the resulting compounds using an Agilent 6890 model GC coupled with an Agilent model 5973 mass spectrometer (MS) as previously described (1). All the identifications were made by comparison to the GC retention times and the mass spectral fragmentation profiles of commercial standards (Sigma-Aldrich, St. Louis, MO) that were similarly analyzed or by comparison with the National Institute of Standards and Technology Mass Spectral Library, version 2.0a.…”

Section: Chemicalsmentioning

confidence: 99%

Proteogenomic Elucidation of the Initial Steps in the Benzene Degradation Pathway of a Novel Halophile, Arhodomonas sp. Strain Rozel, Isolated from a Hypersaline Environment

Dalvi

Azetsu

Patrauchan

et al. 2012

Appl Environ Microbiol

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bLately, there has been a special interest in understanding the role of halophilic and halotolerant organisms for their ability to degrade hydrocarbons. The focus of this study was to investigate the genes and enzymes involved in the initial steps of the benzene degradation pathway in halophiles. The extremely halophilic bacteria Arhodomonas sp. strain Seminole and Arhodomonas sp. strain Rozel, which degrade benzene and toluene as the sole carbon source at high salinity (0.5 to 4 M NaCl), were isolated from enrichments developed from contaminated hypersaline environments. To obtain insights into the physiology of this novel group of organisms, a draft genome sequence of the Seminole strain was obtained. A cluster of 13 genes predicted to be functional in the hydrocarbon degradation pathway was identified from the sequence. Two-dimensional (2D) gel electrophoresis and liquid chromatography-mass spectrometry were used to corroborate the role of the predicted open reading frames (ORFs). ORFs 1080 and 1082 were identified as components of a multicomponent phenol hydroxylase complex, and ORF 1086 was identified as catechol 2,3-dioxygenase (2,3-CAT). Based on this analysis, it was hypothesized that benzene is converted to phenol and then to catechol by phenol hydroxylase components. The resulting catechol undergoes ring cleavage via the meta pathway by 2,3-CAT to form 2-hydroxymuconic semialdehyde, which enters the tricarboxylic acid cycle. To substantiate these findings, the Rozel strain was grown on deuterated benzene, and gas chromatography-mass spectrometry detected deuterated phenol as the initial intermediate of benzene degradation. These studies establish the initial steps of the benzene degradation pathway in halophiles. Many hypersaline environments, such as natural saline lakes, salt flats, solar salterns, saline industrial effluents, oil fields, and salt marshes, have been shown to be contaminated with high levels of petroleum hydrocarbons. Among these, oil fields pose a special problem due to their sheer numbers worldwide and due to their high salinity caused by produced water (salty brackish water) generated during oil and natural gas extraction. Produced water is highly saline and contains a complex mixture of hydrocarbons, including alkanes (linear or branched), cycloalkanes, mono-and polyaromatics, asphaltenes, heavy metals, and resins.The ability of microorganisms to degrade aromatic hydrocarbons in terrestrial and marine environments has been studied extensively under oxic conditions (28,43,44,50,56). On the other hand, little is known about hydrocarbon degradation in hypersaline environments. Bioremediation of polluted hypersaline wastewaters and other environments with nonhalophilic microorganisms is difficult because salt inhibits their growth and the degradation of hydrocarbons (41, 57). Therefore, the cleanup of such environments can only be accomplished by stimulating the growth of indigenous microorganisms capable of degrading petroleum hydrocarbons or through the bioaugmentation of halophilic...

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Anaerobic Metabolism of Biodiesel and Its Impact on Metal Corrosion

Cited by 89 publications

References 33 publications

Fuel Characteristics of Biodiesel Produced from a High-Acid Oil from Soybean Soapstock by Supercritical-Methanol Transesterification

Fuel Characteristics of Biodiesel Produced from a High-Acid Oil from Soybean Soapstock by Supercritical-Methanol Transesterification

Influence of the Anaerobic Biodegradation of Different Types of Biodiesel on the Natural Attenuation of Benzene

Proteogenomic Elucidation of the Initial Steps in the Benzene Degradation Pathway of a Novel Halophile, Arhodomonas sp. Strain Rozel, Isolated from a Hypersaline Environment

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