Anaerobic digestion performance and microbial community structure of corn stover in three-stage continuously stirred tank reactors

Liu, Yue; Wachemo, Akiber Chufo; Yuan, Hairong

doi:10.1016/j.biortech.2019.121339

Cited by 59 publications

(27 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…An effluent tank can be installed between the stages in order to control process conditions, namely, the recirculation ratio and the flow to the second stage. The utilization of effluent instead of chemicals for pH control is a benefit for costs and sustainability [32,73].…”

Section: Recirculation Systemsmentioning

confidence: 99%

Role of Microbial Hydrolysis in Anaerobic Digestion

2020

View full text Add to dashboard Cite

There is a growing need of substrate flexibility for biobased production of energy and value-added products that allows the application of variable biodegradable residues within a circular economy. It can be used to balance fluctuating energy provision of other renewable sources. Hydrolysis presents one of the biggest limitations during anaerobic digestion. Methods to improve it will result in broader process applicability and improved integration into regional material cycles. Recently, one focus of anaerobic digestion research has been directed to systems with a separate hydrolysis–acidogenesis stage as it might be promised to improve process performance. Conditions can be adjusted to each class of microorganisms individually without harming methanogenic microorganisms. Extensive research of separate biomass pretreatment via biological, chemical, physical or mixed methods has been conducted. Nevertheless, several methods lack economic efficiency, have a high environmental impact or focus on specific substrates. Pretreatment via a separate hydrolysis stage as cell-driven biotransformation in a suspension might be an alternative that enables high yields, flexible feeding and production, and a better process control. In this review, we summarize existing technologies for microbial hydrolytic biotransformation in a separate reactor stage and the impacts of substrate, operational parameters, combined methods and process design as well as remaining challenges.

show abstract

Section: Recirculation Systemsmentioning

confidence: 99%

Role of Microbial Hydrolysis in Anaerobic Digestion

2020

View full text Add to dashboard Cite

show abstract

“…All these aspects have made amplicon-sequencing approaches extremely popular and led to a multitude of studies investigating all kinds of habitats. Among them there are the microbiota of humans [7], aquatic ecosystems [8], soils [9], deadwood [10], wastewater treatment systems [11] and anaerobic digesters [12][13][14], to name only a few. Although most of these studies are focused on prokaryotes by targeting the 16S rRNA genes of bacteria and/or archaea, also other structural genes like 18S rRNA [15], 28S rRNA [16,17] and ITS [18], or different functional genes [19,20] are commonly used.…”

Section: Introductionmentioning

confidence: 99%

CoMA – an intuitive and user-friendly pipeline for amplicon-sequencing data analysis

et al. 2020

View full text Add to dashboard Cite

In recent years, there has been a veritable boost in next-generation sequencing (NGS) of gene amplicons in biological and medical studies. Huge amounts of data are produced and need to be analyzed adequately. Various online and offline analysis tools are available; however, most of them require extensive expertise in computer science or bioinformatics, and often a Linux-based operating system. Here, we introduce “CoMA–Comparative Microbiome Analysis” as a free and intuitive analysis pipeline for amplicon-sequencing data, compatible with any common operating system. Moreover, the tool offers various useful services including data pre-processing, quality checking, clustering to operational taxonomic units (OTUs), taxonomic assignment, data post-processing, data visualization, and statistical appraisal. The workflow results in highly esthetic and publication-ready graphics, as well as output files in standardized formats (e.g. tab-delimited OTU-table, BIOM, NEWICK tree) that can be used for more sophisticated analyses. The CoMA output was validated by a benchmark test, using three mock communities with different sample characteristics (primer set, amplicon length, diversity). The performance was compared with that of Mothur, QIIME and QIIME2-DADA2, popular packages for NGS data analysis. Furthermore, the functionality of CoMA is demonstrated on a practical example, investigating microbial communities from three different soils (grassland, forest, swamp). All tools performed well in the benchmark test and were able to reveal the majority of all genera in the mock communities. Also for the soil samples, the results of CoMA were congruent to those of the other pipelines, in particular when looking at the key microbial players.

show abstract

“…Enabling the utilization of straw as energy is an effective way, not only, to potentially solve the problem of environmental pollution caused by straw, but also to ease the world energy crisis. As anaerobic digestion (AD) can degrade the organic components in biomass under mild conditions and produce a large amount of biogas, with methane as the main component, AD is considered to be one of the most suitable and promising biomass energy utilization technologies [3] .…”

Section: Introductionmentioning

confidence: 99%

Biogas and volatile fatty acid production during anaerobic digestion of straw, cellulose, and hemicellulose with analysis of microbial communities and functions

Liu

Zuo

Peng

et al. 2020

Preprint

View full text Add to dashboard Cite

Background Anaerobic digestion (AD) is a promising method for straw treatment, but the complex composition and structure of straw limit AD efficiency and methane production. The main biodegradable components of straw are cellulose and hemicellulose. Because of the different chemical structures and physicochemical properties, the performance of AD of cellulose and hemicellulose is different, thus it’s also different from that of straw. Research on the similarities and differences of AD of straw, cellulose and hemicellulose is helpful to clarify the law of anaerobic digestion of straw and provide theoretical basis for further improving the efficiency of anaerobic digestion. However, there are very few studies on AD using cellulose and hemicellulose as raw materials. Results Rice straw (RS), cellulose, and hemicellulose were used as raw materials to study biogas production performance and changes in the volatile fatty acids (VFAs). Further, microbial communities and genetic functions were analyzed separately for each material. The biogas production potential of RS, cellulose, and hemicellulose was different, with cumulative biogas production of 620.64, 412.50, and 283.75 mL/g·VS− 1, respectively. The methane content of the biogas produced from cellulose and hemicellulose was approximately 10% higher than that produced from RS after the methane content stabilized. Biogas production and the methane content of RS stabilized more quickly than that of cellulose and hemicellulose. The accumulation of VFAs occurred in the early stage of anaerobic digestion in all the three materials, and the main volatile fatty acid component of RS was acetic acid, whereas that of cellulose and hemicellulose was propionic acid. The cumulative amount of VFAs in both cellulose and hemicellulose was relatively higher than that in RS, and the accumulation time was 12 and 14 days longer, respectively. When anaerobic digestion progressed to a stable stage, Clostridium was the dominant bacterial genus in all three AD systems, and the abundance of Ruminofilibacter was higher during anaerobic digestion of RS. Genetically, AD of all the three materials proceeded mainly via aceticlastic methanogenesis, with similar functional components. Conclusion The biogas and VFAs production during AD of RS, cellulose, and hemicellulose showed marked differences. But when the AD progressed to the stable stage, there was no significant difference in microbial community and genetic function. Specifically, the biogas production potential of cellulose and hemicellulose was greater than that of RS. The accumulation of VFAs in the three AD systems occurred in the early stages. The main component of VFA that accumulated in RS was acetic acid, while the major component of VFAs accumulated in cellulose and hemicellulose digestions was propionic acid. At the stable stage, Clostridium was the dominant bacterial genus in all three AD systems. The AD of all the three materials proceeded mainly via aceticlastic methanogenesis, with similar components of gene functions.

show abstract

Anaerobic digestion performance and microbial community structure of corn stover in three-stage continuously stirred tank reactors

Cited by 59 publications

References 42 publications

Role of Microbial Hydrolysis in Anaerobic Digestion

Role of Microbial Hydrolysis in Anaerobic Digestion

CoMA – an intuitive and user-friendly pipeline for amplicon-sequencing data analysis

Biogas and volatile fatty acid production during anaerobic digestion of straw, cellulose, and hemicellulose with analysis of microbial communities and functions

Contact Info

Product

Resources

About