This study was aimed to explore the effect of elastic filler on pollutant removal in each compartment of anaerobic baffled reactor (ABR), ABR with elastic filler, and ABR without elastic filler were compared. The result showed that elastic filler increased the removal rate of chemical oxygen demand (COD) and suspended solid (SS) in the first compartment, decreased the removal rate of COD and SS in the second and third compartments, and had little effect on the removal rate of COD and SS in the fourth compartment. Elastic filler increased the increase rate of ammonia nitrogen in the first and second compartments, decreased the increase rate of ammonia nitrogen in the third and fourth compartments; elastic filler had little effect on the nitrate nitrogen, nitrite nitrogen, total nitrogen and total phosphorus in each compartment of ABR. In general, elastic filler could improve the removal rate of COD and SS of ABR but had little effect on the increase rate of ammonia nitrogen, the removal rate of nitrate nitrogen, nitrite nitrogen, total nitrogen and total phosphorus. Elastic filler slightly increased the concentration of formic acid, acetic acid, propionic acid, and butyric acid in the first compartment of ABR, and slightly decreased the sum of formic acid and acetic acid in the second, hird and fourth compartments. Elastic filler did not change the composition of dissolved organic matter (DOM)in each compartment of ABR, but changed the kinds and contents of aromatic proteins, soluble microbial products and humic acids in each compartment of ABR. Elastic filler had little effect on ABR in the removal of aromatic proteins, but they could improve the removal rate of soluble microbial products and humicacids. Elastic filler slightly increased the degree of DOM humification (or maturity) in ABR effluent, but did not change the main source of humus like substance and DOM in ABR effluent.Sustainability 2020, 12, 2325 2 of 16 suspended solids in the sewage [8] but also effectively kill the pathogenic bacteria in the sewage and fully retain the nitrogen and phosphorus in the sewage [9], so as to reduce the amount of fertilizer, reduce the non-point source pollution caused by fertilizer application, and improve the yield of crops. However, the chemical oxygen demand (COD)and suspended solid (SS)of ABR effluent are high [10][11][12], which not only cannot meet the water quality requirements of all crop irrigation but also improves the operation load of the subsequent treatment process.Elastic filler has the advantages of light weight, stable performance, and low price. Adding elastic filler to ABR can increase its biomass and retention capacity, thus improving its removal effect on COD and SS. Wang Binkeet al. [13] showed that the removal efficiency of COD by ABR (five compartments) without elastic filler and ABR (four compartments) with elastic filler was similar. Yu Yaqin et al. [14] showed that elastic filler accelerated the acclimation of granular sludge in ABR and shortened the start-up time of ABR. Huang Jiguo et al. [...