Anaerobic Dehalogenation of Organohalide Contaminants in the Marine Environment

Häggblom, Max M.; Ahn, Young Beom; Fennell, Donna E.; Kerkhof, Lee J.; Rhee, Sang Dal

doi:10.1016/s0065-2164(03)53002-7

Cited by 41 publications

(18 citation statements)

References 91 publications

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“…This fact suggests the possibility that members of the "Dehalococcoides" group more diverse than those described so far exist and play roles in the dechlorination of PCDD/Fs in the environment. In addition, since dehalorespiration is a widespread property among different phyla of bacteria (31,40,41,53), there is a need to elucidate whether bacteria belonging to phyla other than Chloroflexi are involved in the dechlorination of PCDD/Fs.…”

Section: Discussionmentioning

confidence: 99%

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Phylogenetic Characterization of a Polychlorinated-Dioxin- Dechlorinating Microbial Community by Use of Microcosm Studies

Yoshida

Takahashi

Hiraishi

2005

Appl Environ Microbiol

131

133

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Microcosms capable of reductive dechlorination of polychlorinated dibenzo-p-dioxins/dibenzofurans (PCDD/Fs) were constructed in glass bottles by seeding them with a polluted river sediment and incubating them anaerobically with an organic medium. All of the PCDD/F congeners detected were equally reduced without the accumulation of significant amounts of less-chlorinated congeners as the intermediate or end products. Alternatively, large amounts of catechol and salicylic acid were produced in the upper aqueous phase. Thus, the dechlorination of PCDD/Fs and the oxidative degradation of the dechlorinated products seemed to take place simultaneously in the microcosm. Denaturing gel gradient electrophoresis and clone library analyses of PCR-amplified 16S rRNA genes from the microcosm showed that members of the phyla Firmicutes, Proteobacteria, and Bacteroidetes predominated. A significant number of Chloroflexi clones were also detected. Quantitative real-time PCR with specific primer sets showed that the 16S rRNA genes of a putative dechlorinator, "Dehalococcoides," and its relatives accounted for 0.1% of the total rRNA gene copies of the microcosm. Most of the clones thus obtained formed a cluster distinct from the typical "Dehalococcoides" group. Quinone profiling indicated that ubiquinones accounted for 18 to 25% of the total quinone content, suggesting the coexistence and activity of ubiquinone-containing aerobic bacteria. These results suggest that the apparent complete dechlorination of PCDD/Fs found in the microcosm was due to a combination of the dechlorinating activity of the "Dehalococcoides"-like organisms and the oxidative degradation of the dechlorinated products by aerobic bacteria with aromatic hydrocarbon dioxygenases.

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Section: Discussionmentioning

confidence: 99%

“…The biological significance and potential for biotechnological applications of microbial reductive dehalogenation and dehalorespiration have been well reviewed (31,40,41,53). Reductive dechlorination of PCDD/Fs has been demonstrated in anaerobic mixed cultures from polluted sediments, sludge, and soils (1-3, 9, 10-12, 15, 27, 28, 36, 45, 65).…”

mentioning

confidence: 99%

Phylogenetic Characterization of a Polychlorinated-Dioxin- Dechlorinating Microbial Community by Use of Microcosm Studies

Yoshida

Takahashi

Hiraishi

2005

Appl Environ Microbiol

131

133

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“…Thus, the problem of how to remedy organohalogen pollution is central to environmental science and technology. Harnessing microbial reductive dehalogenation may offer scientifically sound and costeffective bioremediation procedures, because this anaerobic process may work more efficiently than aerobic biodegradation in removing halogen atoms from (poly)halogenated compounds 54,61,80,96,109) . During the past decade, a number of dehalorespiring microorganisms, including a unique group of dehalorespirers, "Dehalococcoides", have been isolated and characterized from phylogenetic, physiologic, and genetic points of view 81,113) .…”

Section: Introductionmentioning

confidence: 99%

Biodiversity of Dehalorespiring Bacteria with Special Emphasis on Polychlorinated Biphenyl/Dioxin Dechlorinators

Hiraishi

2008

Microb. Environ.

102

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A wide variety of haloorganic compounds undergo reductive dehalogenation by certain anaerobic microorganisms. Metabolic reductive dehalogenation is coupled with energy-conserving respiratory electron transport in which a halogenated compound is used as the terminal electron acceptor, the biological process called dehalorespiration or halorespiration. Dehalorespiring bacteria may play important roles in the geochemical cycle with organohalogens in nature and have great promise in their application to the bioremediation of haloorganic contaminants derived from anthropogenic sources. During the past decade, a number of dehalorespiring microorganisms, including a unique group of strictly dehalorespiring bacteria, "Dehalococcoides", have been isolated and characterized at phylogenetic, physiologic, and genetic levels. Also, new perspectives of dehalorespiring bacteria have emerged based on information about genomics and molecular microbial ecology. This review article focuses on up-to-date knowledge of the biodiversity of dehalorespiring bacteria and reductively dehalogenating microbial consortia with special emphasis on those capable of transforming polychlorinated biphenyls and dioxins.

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“…Briefly, notwithstanding consortia abilities, biodegradation is theoretically proportional to energy yield. However, as explained above, microbial degradation of persistent pesticides tends to increase with decreasing redox potential when dehalogenation processes involving anaerobic enzymes are involved: thus biotransformation of organohalide contaminants in situ will vary as a function of the redox conditions within the electron acceptor profile (Häggblom et al, 2003b;Kuhn et al, 1990;Colberg, 1991).…”

Section: In Situ Observations Of Consortia Biodegradation Efficiencymentioning

confidence: 99%

How Early Diagenesis Reveals in Situ Biodegradation of Herbicides in Sediment

Delmotte¹,

Macarie²,

Dolfing³

et al. 2011

Herbicides and Environment

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Anaerobic Dehalogenation of Organohalide Contaminants in the Marine Environment

Cited by 41 publications

References 91 publications

Phylogenetic Characterization of a Polychlorinated-Dioxin- Dechlorinating Microbial Community by Use of Microcosm Studies

Phylogenetic Characterization of a Polychlorinated-Dioxin- Dechlorinating Microbial Community by Use of Microcosm Studies

Biodiversity of Dehalorespiring Bacteria with Special Emphasis on Polychlorinated Biphenyl/Dioxin Dechlorinators

How Early Diagenesis Reveals in Situ Biodegradation of Herbicides in Sediment

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