Anaerobic Degradation of Propanil in Soil and Sediment Using Mixed Bacterial Culture

Oanh, Nguyễn Thị; Duc, Ha Danh

doi:10.1007/s00284-021-02419-7

Cited by 7 publications

(3 citation statements)

References 37 publications

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“…The degradation rate of propanil reached 60% at 2 h and the highest degradation rate of 84% at 8 h. Furthermore, the degradation product of propanil by AmiH52 was determined as 3,4-dichloroaniline (3,4-DCA), which is consistent with previous studies (Herrera-Gonzalez et al 2013 ; Zhang et al 2011 , 2019 ). In contrast, a mixed culture of four bacterial strains, which were isolated from the sediment slurry, transformed 90% of propanil within 10 days in liquid media (Oanh and Duc 2021 ). Another study reported that 81% of propanil was degraded after 100 days of incubation in an anaerobic soil environment (Pettigrew et al 1985 ).…”

Section: Discussionmentioning

confidence: 99%

Characterization of a novel amidohydrolase with promiscuous esterase activity from a soil metagenomic library and its application in degradation of amide herbicides

Sun,

Chen,

Peng

et al. 2024

Environ Sci Pollut Res

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Amide herbicides have been extensively used worldwide and have received substantial attention due to their adverse environmental effects. Here, a novel amidohydrolase gene was identified from a soil metagenomic library using diethyl terephthalate (DET) as a screening substrate. The recombinant enzyme, AmiH52, was heterologously expressed in Escherichia coli and later purified and characterized, with the highest activity occurring at 40 ℃ and pH 8.0. AmiH52 was demonstrated to have both esterase and amidohydrolase activities, which exhibited highly specific activity for p-nitrophenyl butyrate (2669 U/mg) and degrading activity against several amide herbicides. In particular, it displayed the strongest activity against propanil, with a high degradation rate of 84% at 8 h. A GC–MS analysis revealed that propanil was transformed into 3,4-dichloroaniline (3,4-DCA) during this degradation. The molecular interactions and binding stability were then analyzed by molecular docking and molecular dynamics simulation, which revealed that several key amino acid residues, including Tyr164, Trp66, Ala59, Val283, Arg58, His33, His191, and His226, are involved in the specific interactions with propanil. This study provides a function-driven screening method for amide herbicide hydrolase from the metagenomic libraries and a promising propanil-degrading enzyme (AmiH52) for potential applications in environmental remediation.

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Section: Discussionmentioning

confidence: 99%

Characterization of a novel amidohydrolase with promiscuous esterase activity from a soil metagenomic library and its application in degradation of amide herbicides

Sun,

Chen,

Peng

et al. 2024

Environ Sci Pollut Res

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“…1.0 g of soil collected from every single experiment was diluted with sterile distilled water (1:10; w/v), from which bacterial DNA was isolated using the UltraCleanTM Soil DNA Isolation Kit (Mo Bio Laboratories, Inc., Solana Beach, CA, USA). The relative abundance of the bacterial community in soil was determined through Illumina MiSeq sequencing of 16 S rRNA genes as described in a previous study (Oanh & Duc, 2021). The relative abundances of the bacterial species in the soil slurries were determined by sequencing 16 S rRNA genes using an Illumina MiSeq bench-top sequencer.…”

Section: Diversity and Relative Abundance Of Soil Bacterial Communitymentioning

confidence: 99%

Effects of augmentation with \(\textit{Bacillus}\) sp. DT1 on carbonfuran degradation and bacterial community in soil

Duc

Huy

Thanh

et al. 2022

AJB

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Carbofuran is frequently applied to crop fields to combat insects globally causing serious environmental pollution. In this study, the enhanced degradation of carbofuran in soil by augmentation with Bacillus sp. DT1 was determined. The results showed that the augmentation with the bacterial strain increased the degradation by almost 30% compared to attenuation after 30 days at an initial concentration of 10 mg/kg dry soil. The half-life values of the insecticide were 41.6 and 17.9 days for attenuation and augmentation, respectively. Moreover, the augmentation with Bacillus sp. DT1 significantly increased sequence numbers of 16 S rRNA in soil compared to the initial stage. However, α-diversity indices, i.e., values of OTUs, ACE, Chao1 and Shannon showed no statistical differences in soil with and without the insecticide addition, with and without augmentation after 30 days. These results showed that carbofuran did not inhibit or stimulate the growth of soil bacteria at 10 mg/kg dry soil.

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“…16S rRNA and ITS sequences of distinct regions (16S V3-V4, ITS1) were amplified using specific primers with a barcode. The forward primer 338F (5 -ACTCCTACGGGAGGCAG CAG-3 ) and the reverse primer 806R (5 -GGACTACHVGGGTWTCTAAT-3 ) were used to amplify the V3-V4 region of 16S rRNA genes [25]. The ITS1 region of fungal nuclear DNA was amplified with primers ITS1F (5 -CTTGGTCATTTAGAGGAAGTAA-3 ) and ITS2 (5 -GCTGCGTTCTTCATCGATGC-3 ) [26].…”

Section: Gene Amplicons and Illumina Miseq Sequencingmentioning

confidence: 99%

Application of Polyaluminium Chloride Coagulant in Urban River Water Treatment Influenced the Microbial Community in River Sediment

Liu

Zhuang²,

Wang

2021

Water

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Polyaluminium chloride (PAC) has been widely used as a chemical coagulant in water treatment. However, little is known about the impact of PAC performance on the microbial community in sediments. In this study, the archaeal, bacterial, and fungal communities in urban river sediments with and without PAC treatment were investigated. Prokaryotic diversity decreased at the PAC addition site (A2) and increased along with the river flow (from A3 to A4), while eukaryotic diversity was the opposite. The abundance of core microbiota showed a similar trend. For example, the dominant Proteobacteria presented the highest relative abundance in A1 (26.8%) and the lowest in A2 (15.3%), followed by A3 (17.5%) and A4 (23.0%). In contrast, Rozellomycota was more dominant in A2 (56.6%) and A3 (58.1%) than in A1 (6.2%) and A4 (16.3%). Salinity, total dissolved solids, and metal contents were identified as the key physicochemical factors affecting the assembly of core microorganisms. The predicted functions of archaea and fungi were mainly divided into methane cycling and saprotrophic nutrition, respectively, while bacterial function was more diversified. The above findings are helpful to enhance our understanding of microorganism response to PAC and have significance for water treatment within the framework of microecology.

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Anaerobic Degradation of Propanil in Soil and Sediment Using Mixed Bacterial Culture

Cited by 7 publications

References 37 publications

Characterization of a novel amidohydrolase with promiscuous esterase activity from a soil metagenomic library and its application in degradation of amide herbicides

Characterization of a novel amidohydrolase with promiscuous esterase activity from a soil metagenomic library and its application in degradation of amide herbicides

Effects of augmentation with \(\textit{Bacillus}\) sp. DT1 on carbonfuran degradation and bacterial community in soil

Application of Polyaluminium Chloride Coagulant in Urban River Water Treatment Influenced the Microbial Community in River Sediment

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