Anaerobic degradation of long-chain alkylamines by a denitrifying Pseudomonas stutzeri

Abstract

The anaerobic degradation of tetradecylamine and other long-chain alkylamines by a newly isolated denitrifying bacterium was studied. Strain ZN6 was isolated from a mixture of soil and active sludge and was identified as representing Pseudomonas stutzeri, based on partial 16S rRNA gene sequence analysis. Strain ZN6 was a mesophilic, motile, Gram-negative rod-shaped bacterium and was able to grow on a variety of compounds including even-numbered primary fatty amines with alkyl chains ranging from C(4) to C(18) coupled to nitrate reduction. Alkylamines were used as sole carbon, energy and nitrogen source and were completely mineralized. Nitrate was dissimilated by ZN6 to nitrite. When strain ZN6 was grown under nitrate limitation, nitrite was slowly dissimilated further. When cocultivated with the complete denitrifier Castellaniella defragens ZN3, anaerobic degradation under denitrifying of alkylamines by strain ZN6 was slightly faster. Strain ZN3 is a complete denitrifier, unable to convert tetradecylamine, and was copurified from the same enrichment culture as strain ZN6. The proposed pathway for the degradation of alkylamines in strain ZN6 starts with C-N cleavages to alkanals and further oxidation to the corresponding fatty acids.

Isolation and characterization of homocholine-degrading Pseudomonas sp. strains A9 and B9b

“…Bacteria belonging to this genus are engaged in important metabolic activities in the environment, including element cycling and the degradation of biogenic and xenobiotic pollutants (Timmis 2002). They play a key role in the metabolisms of quaternary ammonium compounds such as choline (Nagasawa et al 1976), c-butyrobetaine (Miura-Fraboni and Englard 1983), trimethylamino-1-butanol (Hassan et al 2007), 2,3-dihydroxypropyl-trimethylammonium (Kaech et al 2005), and many other long-chain quaternary ammonium compounds (Nishihara et al 2000;Nguyen et al 2008;Takenaka et al 2007;van Ginkel et al 1992). Additionally, we reported herein the isolation of two Pseudomonas strains able to degrade homocholine and to use it as sole carbon and nitrogen source.…”

Biofilm Interaction Mapping and Analysis (BIMA) of Interspecific Interactions in Pseudomonas Co-culture Biofilms

“…Pseudomonas stutzeri , a model denitrifying pseudomonas, can grow in diverse conditions ( Lalucat et al, 2006 ) and its use has been demonstrated in a number of bioremediation processes, including phenol ( Jiang et al, 2014 ), carbon tetrachloride ( Sepúlveda-Torres et al, 2002 ), uranium ( Han et al, 2012 ), polycyclic aromatic hydrocarbons ( Moscoso et al, 2012a ), and diesel oil ( Kaczorek et al, 2012 ). Additionally, P. stutzeri has been identified in and used in a number of consortia-based applications, such as bioremediation ( Nguyen et al, 2008 ; Brodie et al, 2011 ; Gałazka et al, 2012 ), wastewater denitrification ( Clarens et al, 1998 ; Liu et al, 2006 ), aquaculture water quality management ( Erna et al, 2013 ; Deng et al, 2014 ), as a plant growth promoter for the biocontrol of phytopathogens ( Cottyn et al, 2009 ; Shen et al, 2013 ), and manufacturing/municipal waste management ( Miyahara et al, 2012 ; Moscoso et al, 2012b ). The use of P. stutzeri biofilms for various bioremediation efforts has potential benefits in terms of activity and yields for copper removal ( Badar et al, 2013 ), drinking water denitrification ( Lazarova et al, 1992 ) and naphthalene ( Zhao et al, 2016 ), and phenol ( Viggiani et al, 2006 ) degradation.…”

Biofilm Interaction Mapping and Analysis (BIMA): A tool for deconstructing interspecific interactions in co-culture biofilms

“…Pseudomonas stutzeri, a model denitrifying pseudomonas, can grow in diverse conditions (13) and its use has been demonstrated in a number of bioremediation processes, including phenol (14), carbon tetrachloride (15), uranium (16), polycyclic aromatic hydrocarbons (17) and diesel oil (18). Additionally, P. stutzeri has been discovered and used in a number of consortia based applications, such as bioremediation (19)(20)(21), wastewater denitrification (22,23), aquaculture water quality management (24,25), as a plant growth promoter for the biocontrol of phytopathogens (26,27) and manufacturing/municipal waste management (28,29). The use of P. stutzeri biofilms for various bioremediation efforts has potential benefits in terms of activity and yields for copper removal (30), drinking water denitrification (31) and naphthalene (32) and phenol (33) degradation.…”