Anaerobic cellulolytic microbial communities decomposing the biomass of Anabaena variabilis

Петрова, Е. В.; Егорова, М.А.; Piskunkova, N. F.; Kozhevin, P. A.; Нетрусов, А.И.; Цавкелова, Е. А.

doi:10.1134/s0026261717060133

Cited by 3 publications

(5 citation statements)

References 26 publications

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“…Strain 10-4 is capable of producing IAA, siderophores, and fix atmospheric N2 [32]. The cells of BS were cultured in a liquid Luria-Bertani (LB) medium for 24 h (37 • C, 180 rpm) [44]. To produce inoculum, freshly obtained liquid bacterial culture containing 10 9 cells mL −1 was diluted down to 10 5 cells mL −1 using sterile water.…”

Section: Bacterial Strain and Inoculum Preparationmentioning

confidence: 99%

The Contribution of Hormonal Changes to the Protective Effect of Endophytic Bacterium Bacillus subtilis on Two Wheat Genotypes with Contrasting Drought Sensitivities under Osmotic Stress

Lastochkina,

Yuldashev,

Avalbaev

et al. 2023

Microorganisms

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A comparative analysis was conducted to evaluate the effects of seed priming with endophytic bacterium Bacillus subtilis 10-4 (BS) on the hormonal system and cell wall tolerance (lipid peroxidation (LPO), electrolyte leakage (EL), and root lignin deposition) of two Triticum aestivum L. (wheat) varieties with contrasting drought sensitivities (Ekada 70—drought-tolerant (DT); Salavat Yulaev—drought-sensitive (DS)) under normal conditions and 12% polyethylene glycol-6000 (PEG)-induced osmotic stress. The results showed that under normal conditions, the growth stimulation in wheat plants by BS was attributed to changes in the hormonal balance, particularly an increase in endogenous indole-3-acetic acid (IAA) accumulation. However, under stress, a significant hormonal imbalance was observed in wheat seedlings, characterized by a pronounced accumulation of abscisic acid (ABA) and a decrease in the levels of IAA and cytokinins (CK). These effects were reflected in the inhibition of plant growth. BS exhibited a protective effect on stressed plants, as evidenced by a significantly lower amplitude of stress-induced changes in the hormonal system: maintaining the content of IAA at a level close to the control, reducing stress-induced ABA accumulation, and preventing CK depletion. These effects were further reflected in the normalization of growth parameters in dehydrated seedlings, as well as a decrease in leaf chlorophyll degradation, LPO, and EL, along with an increase in lignin deposition in the basal part of the roots in both genotypes. Overall, the findings demonstrate that BS, producing phytohormones, specifically IAA and ABA, had a more pronounced protective effect on DT plants, as evidenced by a smaller amplitude of stress-induced hormonal changes, higher leaf chlorophyll content, root lignin deposition, and lower cell membrane damage (LPO) and permeability (EL) compared to DS plants.

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Section: Bacterial Strain and Inoculum Preparationmentioning

confidence: 99%

The Contribution of Hormonal Changes to the Protective Effect of Endophytic Bacterium Bacillus subtilis on Two Wheat Genotypes with Contrasting Drought Sensitivities under Osmotic Stress

Lastochkina,

Yuldashev,

Avalbaev

et al. 2023

Microorganisms

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“…The seeds were supplied by the Chishminsky Breeding Station UFRC RAS (Chishmy, Ufa, Russia). The endophytic bacterium B. subtilis 10-4 (BS) was previously isolated from dryland arable soils of the Republic of Bashkortostan (52 • 36 N 58 • 19 E, Russia) using a classical microbiological method [50], identified using 16S rRNA [44], characterized in detail [44,45], and deposited in the National Bio-Resource Center of the All-Russian Collection of Industrial Microorganisms (VKPM) with registration number B-12988. BS is able to colonize inner wheat tissues (endophyte) and induce plant growth-promoting traits, particularly, the production of auxins, siderophores, catalase and the fixation of atmospheric nitrogen [44].…”

Section: Plant Materials and Bacterial Strainmentioning

confidence: 99%

“…Previously, we found that the pre-sowing seed treatment with the PGPM B. subtilis strain 10-4 had a growth-stimulating and anti-stress effect on various plants, including wheat under the influence of salinity [44,45], drought [46], and a combination of herbicide and drought [47]. It was shown that the protective effect of strain 10-4 is based on its ability to colonize the internal tissues of the host plant (including wheat), produce auxins, fix atmospheric nitrogen, solubilize phosphates, modulate the level of plant phytohormones (in particular, salicylic acid) [48] and the content of photosynthetic pigments, regulate the components of the ascorbate-glutathione complex [49], reduce stress-induced oxidative and osmotic cell damages, and also accelerate the lignification of root cell walls, as demonstrated for bean plants during salinity [50]. Based on the above-mentioned findings, we suggested that this bacterium has the potential to protect wheat plants against Cd toxicity as well.…”

Section: Introductionmentioning

confidence: 99%

Endophytic Plant Growth-Promoting Bacterium Bacillus subtilis Reduces the Toxic Effect of Cadmium on Wheat Plants

et al. 2023

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Heavy metal ions, in particular cadmium (Cd), have a negative impact on the growth and productivity of major crops, including wheat. The use of environmentally friendly approaches, in particular, bacteria that have a growth-stimulating and protective effect, can increase the resistance of plants. The effects of the pre-sowing seed treatment with the plant growth-promoting endophyte Bacillus subtilis 10-4 (BS) on cadmium acetate (Cd)-stressed Triticum aestivum L. (wheat) growth, photosynthetic pigments, oxidative stress parameters, roots’ lignin content, and Cd ions accumulation in plants were analyzed. The results showed that the tested Cd-tolerant BS improved the ability of wheat seeds to germinate in the presence of different Cd concentrations (0, 0.1, 0.5, and 1 mM). In addition, the bacterial treatment significantly decreased the damaging effects of Cd stress (1 mM) on seedlings’ linear dimensions (lengths of roots and shoots), biomass, as well as on the integrity and permeability of the cell walls (i.e., lipid peroxidation and electrolyte leakage) and resulted in reduced H2O2 generation. The pretreatment with BS prevented the Cd-induced degradation of the leaf photosynthetic pigments chlorophyll (Chl) a, Chl b, and carotenoids. Moreover, the bacterial treatment intensified the lignin deposition in the roots under normal and, especially, Cd stress conditions, thereby enhancing the barrier properties of the cell wall. This manifested in a reduced Cd ions accumulation in the roots and in the restriction of its translocation to the aboveground parts (shoots) of the bacterized plants under Cd stress in comparison with non-bacterized controls. Thus, the pre-sowing seed treatment with the endophyte BS may serve as an eco-friendly approach to improve wheat production in Cd-contaminated areas.

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“…Several microalgae have been used to combine biomethane production with biorefinery approaches, for example, Chlamydomonas reinhardtii and Scenedesmus obliquus . There are some reports of T. variabilis biomass conversion into biogas under high temperature using anaerobic digesters without, or in combination with, immobilizing technology to enhance gas production . Methane production yield was recorded at 450 mL g −1 biomass using immobilized methanogenic bacteria and Rhodobacter capsulatus on polymeric matrices in an anaerobic bioreactor .…”

Section: Bioenergy Production Using T Variabilismentioning

confidence: 99%

“…Methane production yield was recorded at 450 mL g −1 biomass using immobilized methanogenic bacteria and Rhodobacter capsulatus on polymeric matrices in an anaerobic bioreactor . In another research cumulative methane yield (64%) was recorded at ~4 mmol g −1 biomass when anaerobic cellulolytic substrate were used together with methanogenic Archaea from genera of Methanoculleus and Methanosarcina Biogas is generated in four successive stages: (i) hydrolysis of biopolymers to monomers, (ii) fermentation (acidogenesis) of amino acids and sugars to intermediary products, (iii) acetogenesis of intermediary products to acetate, CO 2 and hydrogen, and (iv) methanogenesis, which transforms acetate into methane. During anaerobic digestion, hydrolysis is known to be the rate limiting step which needs to be optimized via efficient pre‐treatment technologies.…”

Section: Bioenergy Production Using T Variabilismentioning

confidence: 99%

Bioenergy production using Trichormus variabilis – a review

Abedi

Astaraei

Ghobadian

et al. 2019

Biofuels Bioprod Bioref

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Fossil-fuel processing and consumption contaminates air, soil, and water resources through the release of hazardous chemicals. The harnessing of renewable energy resources and development of sustainable technologies have become prime targets of research and increased investment to protect the environment. The use of bio-based feedstocks in energy production provides a valuable pollutioncurbing pathway with sustainability credentials, especially when wastewater is used to provide the nutrient requirements. The filamentous cyanobacterium Trichormus variabilis has attracted substantial attention from researchers due to its potential for dual industrial functions in bioenergy production and bioremediation. This species can use the power of sunlight energy efficiently to fix atmospheric CO 2 and to generate valuable chemical compounds, such as carbohydrates and fatty acids, which can be converted to biofuels. As it grows in nutrient-rich wastewater (industrial effluent) it can serve as a bioabsorbant and replace costly chemical catalysts and nano-materials traditionally used for the removal of nutrients and metals. However, no recent review has presented the potential for state-of-the-art T. variabilisdriven phycoremediation-bioenergy production systems. This review suggests possible routes from phycoremediation to energy production as a strategy for developing the industrial application of T. variabilis. It brings important research results on this species together and highlights major related challenges and opportunities. It explores the current status of the use of algae in bioremediation and the production of liquid and gaseous fuels utilizing wild-type and mutants of T. variabilis. Finally, key points underlying the potential for future research on optimization of robust technologies for supplying sustainable bioenergy using this organism are presented.

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Anaerobic cellulolytic microbial communities decomposing the biomass of Anabaena variabilis

Cited by 3 publications

References 26 publications

The Contribution of Hormonal Changes to the Protective Effect of Endophytic Bacterium Bacillus subtilis on Two Wheat Genotypes with Contrasting Drought Sensitivities under Osmotic Stress

The Contribution of Hormonal Changes to the Protective Effect of Endophytic Bacterium Bacillus subtilis on Two Wheat Genotypes with Contrasting Drought Sensitivities under Osmotic Stress

Endophytic Plant Growth-Promoting Bacterium Bacillus subtilis Reduces the Toxic Effect of Cadmium on Wheat Plants

Bioenergy production using Trichormus variabilis – a review

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