Anaerobic bacterial populations on normal and diseased human biopsy tissue obtained at colonoscopy

Edmiston, Charles E.; Avant, George R.; Wilson, Felicia

doi:10.1128/aem.43.5.1173-1181.1982

Cited by 49 publications

(10 citation statements)

References 15 publications

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“…All tissue homogenates contained at least 107 anaerobes and 106 aerobes per g, and the most frequently isolated organisms were anaerobic and microaerophilic streptococci and enterobacteria. It is unlikely that the higher counts of aerobes obtained in these two previous studies were due to the use of different techniques; it is more likely that the mucosal flora was altered as a result of preoperative treatment, and Edmiston et al (15) recently suggested that, in colonoscopy specimens, the decreased numbers of anaerobes associated with the mucosa of the distal colon may result from the routine enemas given before investigation. In the present study a wider range of organisms was found, and the flora of each subject was quite distinct when individual groups of organisms were compared.…”

Section: Discussionmentioning

confidence: 88%

“…The mucosal flora may be of importance in maintaining the stability of the fecal flora and may have specific functions (10). Results from studies on the flora of the human colon wall conflict (15, [25][26][27], perhaps because of the difficulties in obtaining suitable material, as biopsies taken during colonoscopic examination of the large bowel are very small and may not be representative (15) and specimens obtained during intestinal surgery from uninvolved tissue (26, 27) may be abnormal due to the clinical condition or as a result of premedication.…”

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confidence: 99%

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Bacterial Populations Associated with Different Regions of the Human Colon Wall

Croucher¹,

Houston²,

Bayliss³

et al. 1983

Appl Environ Microbiol

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The microorganisms associated with the undiseased human colon wall were examined in material obtained from four sudden-death victims. In traffic accident subjects (aged 45 and 16 years) the anaerobe-aerobe ratio was about 10 4 :1 in all areas of the colon examined, whereas in acute heart failure subjects (aged 74 and 46 years) the ratio was as low as 1.2:1. The flora of each individual was distinct and complex. Although the predominant anaerobes isolated were Bacteroides and Fusobacterium spp., which composed over 50% of the flora in some samples, the species isolated (indicated by morphology and glucose fermentation products) varied between individuals. Other major types observed were gram-positive nonsporing rods, including Bifidobacterium spp., and anaerobic cocci (between 8 and 20% of isolates). Clostridia were only isolated in significant numbers from one individual. Scanning electron microscopy showed that most of the organisms were present below the top surface of the mucin layer overlying the mucosa. The use of several different preparative procedures for microscopy showed a complex microbial structure within the mucus, but major variations in the bacterial populations in different areas of the colon were not found. Spiral-shaped organisms up to 60 μm long in the form of double helices were found in two subjects by scanning electron microscopy but were not isolated during the parallel bacteriological investigation. The differences between this and previous studies are discussed in relation to experimental procedures and also in contrast to results with animals that showed a particularly specialized flora associated with the colon wall.

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Section: Discussionmentioning

confidence: 88%

mentioning

confidence: 99%

Bacterial Populations Associated with Different Regions of the Human Colon Wall

Croucher¹,

Houston²,

Bayliss³

et al. 1983

Appl Environ Microbiol

View full text Add to dashboard Cite

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“…Fusobacteria (17) (20) were the most frequently identified and reported bacteria from malignant tissues of colorectal cancer, our study didn't showed any above mentioned species while we employed candle jar cultivation.…”

Section: Resultsmentioning

confidence: 60%

“…Other most fusobacterial members strictly require anaerobic environment to grow (15) and are associated greatly with cancer tissues than in normal tissues (16). Despite the genera Bacteriodes (17), Leptotrichia species (18)(19) and…”

Section: Resultsmentioning

confidence: 99%

Mucosa-associated Cultivable Aerobic Gut Bacterial Microbiota among Colorectal Cancer Patients Attending at the Referral Hospitals of Amhara Regional State, Ethiopia

Siraj

Byadgelegn

Yassin

et al. 2020

Preprint

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Background: Colorectal cancer is one of the top ten cancer death in the world. Despite an increased prevalence of colorectal cancer has been documented from developing countries, reports on gut microbiota among colorectal cancer patients are none especially in Ethiopia. Therefore, the current study evaluated cultivable aerobic bacterial distributions among malignant tissue of colorectal cancer and its adjacent normal biopsies. Methods: Fifteen CRC patients who were undergoing colorectal cancer resection surgery during April 2017 to February 2018 at Felege Hiwot Referral and University of Gondar Teaching Hospitals were included. Biopsy specimens were taken from malignant and its adjacent normal tissues. Bacterial cultivation, quantification and characterization of saline washed biopsies were performed under aerobic and candle jar conditions. Differences in bacterial microbiota compositions between malignant and normal tissue biopsies were evaluated and analyzed using Microsoft excel 2010 and GraphPad Prism5 statistical software. Results: Fifteen CRC patients were participated with a mean age of 53.8 ± 10.8 years old and majorities (73.3%) of patients were in between the age groups of 40 and 60 years old. The mean ± SD bacterial microbiota of malignant biopsies (3.2x10 5 ± 1.6x10 5 CFU/ml) was significantly fewer than that of adjacent normal tissue biopsies (4.0x10 5 ± 2.2x10 5 CFU/ml). This dysbacteriosis is positively correlated with the occurrence of CRC (p=0.019). Proteobacteria (55.6%), Firmicutes (33.3%) and Fusobacteria (11.1%) were the most frequently isolated phyla from non-malignant biopsies while only Proteobacteria (58.8%) and Firmicutes (41.2%) were from malignant ones. Family level differences were observed among phyla (Firmicutes and Proteobacteria) isolated from the study participants. For instance, the relative abundance of family Bacillaceae from malignant (26%) was lower than the normal biopsies (39%). On other hand, family Enterobacteriaceae was twice more abundant in malignant tissues (45%) than in its matched normal tissues (23%). Furthermore, the family Enterococcaceae (14%) of family Firmicutes was solely isolated from malignant tissue biopsies. Conclusion: The overall microbial composition of normal and malignant tissues was considerably different among the study participants. Further culture independent analysis of mucosal microbiota will provide detail pictures of microbial composition differences and pathogenesis of CRC in Ethiopian settings.

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“…This finding is correlated with an increased excretion in the feces of indole and skatole (16), which are known as Clostridium metabolites (6), but the relationships between these phenomena remain unclear (16). On the other hand, clostridia and other anaerobic bacteria are found in higher numbers in colonic polyps than on the adjacent normal mucosa (7). It is therefore reasonable to assume that clostridia can represent an explanation for these phenomena.…”

Section: Discussionmentioning

confidence: 99%