Anaerobic and hydrogenogenic carbon monoxide-oxidizing prokaryotes: Versatile microbial conversion of a toxic gas into an available energy

Fukuyama, Yuto; Inoue, Masao; Omae, Kimiho; Yoshida, Takashi; Sako, Yoshihiko

doi:10.1016/bs.aambs.2019.12.001

Cited by 38 publications

(38 citation statements)

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“…CO uptake at low concentrations by soils in particular contributes significantly to the global atmospheric CO budget [ 7 ]. In contrast, CO uptake attributed to Ni-dependent CO dehydrogenases has been reported for a phylogenetically more restricted group of Bacteria and Archaea, most of which are thermophilic [ 10 , 18 , 40 , 41 ]. The molecular biology, biochemistry, and physiology of selected isolates have been characterized extensively [ 9 , 10 , 15 , 18 , 42 ], but the ecological significance of Ni-COX remains largely unknown with a few exceptions.…”

Section: Discussionmentioning

confidence: 99%

“…Applications of stable and radioisotopic probing approaches and CO uptake assays have revealed a relatively low diversity for active Ni-COX, but also indicated that CO uptake can contribute significantly to hot spring community metabolism when CO is relatively abundant [ 14 , 20 , 45 ]. Other studies have addressed Ni-COX in sewage sludge [ 46 , 47 ], the use of Ni-COX in microbial fuel cells [ 48 , 49 ] and the prospects for bioconversions of syngas to hydrogen [ 41 ].…”

Section: Discussionmentioning

confidence: 99%

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Anaerobic Carbon Monoxide Uptake by Microbial Communities in Volcanic Deposits at Different Stages of Successional Development on O-yama Volcano, Miyake-jima, Japan

2020

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Research on Kilauea and O-yama Volcanoes has shown that microbial communities and their activities undergo major shifts in response to plant colonization and that molybdenum-dependent CO oxidizers (Mo-COX) and their activities vary with vegetation and deposit age. Results reported here reveal that anaerobic CO oxidation attributed to nickel-dependent CO oxidizers (Ni-COX) also occurs in volcanic deposits that encompass different developmental stages. Ni-COX at three distinct sites responded rapidly to anoxia and oxidized CO from initial concentrations of about 10 ppm to sub-atmospheric levels. CO was also actively consumed at initial 25% concentrations and 25 °C, and during incubations at 60 °C; however, uptake under the latter conditions was largely confined to an 800-year-old forested site. Analyses of microbial communities based on 16S rRNA gene sequences in treatments with and without 25% CO incubated at 25 °C or 60 °C revealed distinct responses to temperature and CO among the sites and evidence for enrichment of known and potentially novel Ni-COX. The results collectively show that CO uptake by volcanic deposits occurs under a wide range of conditions; that CO oxidizers in volcanic deposits may be more diverse than previously imagined; and that Ni-dependent CO oxidizers might play previously unsuspected roles in microbial succession.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Anaerobic Carbon Monoxide Uptake by Microbial Communities in Volcanic Deposits at Different Stages of Successional Development on O-yama Volcano, Miyake-jima, Japan

2020

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“…Hydrogenogenic carbon monoxide (CO) oxidizers are microbes that conserve energy by coupling CO oxidation with hydrogen (H 2 ) production in the following reaction (WGSR; water-gas shift reaction): CO+H 2 O↔CO 2 +H 2 (Fox et al, 1996;Fukuyama et al, 2020). In WGSR, CO is oxidized by a CO dehydrogenase (CODH), which is the only enzyme that oxidizes CO (Xavier et al, 2018), and electrons are transferred to an energy-converting hydrogenase (ECH), in which protons and sodium ions are translocated (Schoelmerich and Müller, 2019).…”

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confidence: 99%

“…In WGSR, CO is oxidized by a CO dehydrogenase (CODH), which is the only enzyme that oxidizes CO (Xavier et al, 2018), and electrons are transferred to an energy-converting hydrogenase (ECH), in which protons and sodium ions are translocated (Schoelmerich and Müller, 2019). These microbes have the potential to produce H 2 , an energy source with a low environmental impact, on an industrial scale from industrial waste gases containing CO (Fukuyama et al, 2020).…”

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confidence: 99%

“…Genetic engineering is regarded as one of the major approaches to establish metabolically manipulated cell lines with the more efficient biocatalytic production of H 2 from CO (Fukuyama et al, 2020). Genetic engineering methods and tools have been developed in P. thermoglucosidasius to delete target genes (Cripps et al, 2009;Bacon et al, 2017) and transform exogenous genes (Taylor et al, 2008;Reeve et al, 2016).…”

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confidence: 99%

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Genetic Engineering of Carbon Monoxide-dependent Hydrogen-producing Machinery in <i>Parageobacillus thermoglucosidasius</i>

et al. 2020

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The metabolic engineering of carbon monoxide (CO) oxidizers has the potential to create efficient biocatalysts to produce hydrogen and other valuable chemicals. We herein applied markerless gene deletion to CO dehydrogenase/energyconverting hydrogenase (CODH/ECH) in the thermophilic facultative anaerobe, Parageobacillus thermoglucosidasius. We initially compared the transformation efficiency of two strains, NBRC 107763 T and TG4. We then disrupted CODH, ECH, and both enzymes in NBRC 107763 T. The characterization of growth in all three disruptants under 100% CO demonstrated that both enzymes were essential for CO-dependent growth with hydrogen production in P. thermoglucosidasius. The present results will become a platform for the further metabolic engineering of this organism.

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Biological Production of Hydrogen

Martins

Pereira

Pita

et al. 2020

Enzymes for Solving Humankind's Problems

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Decrease of greenhouse gases like CO2 has become a key challenge for human kind and the study of the electrocatalytic properties of CO2-reducing enzymes such as formate dehydrogenases is of importance for this goal. In this work we study the covalent bonding of Desulfovibrio vulgaris Hildenborough FdhAB formate dehydrogenase to chemically modified gold and low-density graphite electrodes, using electrostatic interactions for favoring oriented immobilization of the enzyme.Electrochemical measurements show both bioelectrocatalytic oxidation of formate and reduction of CO2 by direct electron transfer. Atomic force microscopy and quartz crystal microbalance characterization, as well as comparison of direct and mediated electrocatalysis, suggest that a compact layer of formate dehydrogenase was anchored to the electrode surface with some crosslinked aggregates. Furthermore, operational stability for CO2 electroreduction to formate by direct electron transfer is shown with approximately 100% faradaic yield.

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Anaerobic and hydrogenogenic carbon monoxide-oxidizing prokaryotes: Versatile microbial conversion of a toxic gas into an available energy

Cited by 38 publications

References 181 publications

Anaerobic Carbon Monoxide Uptake by Microbial Communities in Volcanic Deposits at Different Stages of Successional Development on O-yama Volcano, Miyake-jima, Japan

Anaerobic Carbon Monoxide Uptake by Microbial Communities in Volcanic Deposits at Different Stages of Successional Development on O-yama Volcano, Miyake-jima, Japan

Genetic Engineering of Carbon Monoxide-dependent Hydrogen-producing Machinery in <i>Parageobacillus thermoglucosidasius</i>

Biological Production of Hydrogen

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