Anaerobic and Aerobic Respiration in Yeast: Small-Scale Variations on a Classic Laboratory Activity

Koutsokali, Maria; Valahas, Michael

doi:10.1021/acs.jchemed.9b00994

Cited by 11 publications

(7 citation statements)

References 10 publications

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“…Saccharomyces spp. strains are facultative anaerobic microbes capable of proliferating under both aerobic and anaerobic conditions [33,34], and it was confirmed that SB and SC could grow in livestock manure under all tested conditions. After 24 h incubation, DNA proportion of SB increased from approximately 20-45% under all culture conditions (SB in aerobic condition, p = 0.0419; SB in anaerobic condition, p = 0.0481).…”

Section: Growth Characteristics Of Yeasts In Manure Depending On Cultmentioning

confidence: 61%

Characterisitcs of Saccharomyces boulardii for reducing ammonia emission from livestock manure

et al. 2021

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Ammonia from livestock manure acts as a precursor to produce particulate matter (PM) by reacting with atmospheric chemical components volatilized from various sources. Ammonia itself acts as a toxic substance to human health, and thus has direct or indirect adverse effects on human health. This study aimed to verify the effectiveness and mechanism of action of Saccharomyces boulardii (SB) in reducing the ammonia emission from livestock manure. The specific ability of SB was confirmed through comparative verification with S. cerevisiae (SC) belonging to the same genus. SB and SC could use 50% of ammonia–nitrogen as inorganic nitrogen source in minimal medium. In the control group, the pH level of manure was significantly increased compared to the pH level at 0 h, and the DNA concentration of Proteus mirabilis, which increase the manure pH through ammonia production, was found to increase by 2.7-fold. Significant decrease in pH and proliferation of P. mirabilis was found in SB group compared to control (p < 0.05). The SB group also reduced the amount of ammonia emitted from manure by 25% for 35 days. These results suggested that SB contributed to reducing ammonia emission from manure by reducing pH and inhibiting HAB as well as removing ammonia–nitrogen. Accordingly, SB as a microbiological agent is expected to contribute not only to reduce ammonia emission but also to improve manure quality as a fertilizer.

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Section: Growth Characteristics Of Yeasts In Manure Depending On Cultmentioning

confidence: 61%

Characterisitcs of Saccharomyces boulardii for reducing ammonia emission from livestock manure

et al. 2021

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“…Here we present an undergraduate laboratory experiment that involves labeling of the common baker’s yeast, Saccharomyces cerevisiae , with iron complexes with the goal of introducing cellular biology and coordination chemistry of Fe(III). This experiment shows the relative ease of handling yeast in comparison to other types of cells as acknowledged in previous articles in this Journal describing beer brewing activities, biochemistry principles, or enzymology of yeast proteins . Coordination chemistry and paramagnetic properties of complexes are also a focus of this laboratory experiment.…”

Section: Introductionmentioning

confidence: 61%

Magnetization of Yeast by Labeling with Iron Complexes: An Undergraduate Laboratory Experiment

et al. 2022

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This undergraduate laboratory experiment is designed for first-year general chemistry students with the goal of introducing the brilliant colors and magnetic properties of transition metal complexes within a bioinorganic/cell biology context. In the first laboratory period, a coordination complex formed from ferric chloride and maltol is prepared (Fe(maltol)3). The intense red color of the complex is noted, and the UV–vis spectra of the ligand and complex are compared. Yeast cells are incubated with ferric chloride and with Fe(maltol)3. In the second laboratory period, the yeast cells treated with the ferric chloride or Fe(maltol)3 are isolated and placed over ring magnets to study whether the yeast have paramagnetic properties. Yeast viability studies are done to compare the toxicity of Fe(maltol)3 and FeCl3. Students are asked to predict the magnetic properties of iron complexes of different spin and oxidation states and to consider the basis for iron uptake into yeast cells.

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“…This trend continues until approximately 19 h after the initiation of the culture, when a rapid decrease in CO 2 is observed. The exact reason is not clear, but this could be because of the lack of sufficient nutrients and oxygen ( Tervasmäki et al, 2018 ; Koutsokali and Valahas 2020 ). At about the time point of 16 h, dissolved oxygen level reaches zero.…”

Section: Resultsmentioning

confidence: 99%

A novel approach to noninvasive monitoring of dissolved carbon dioxide in small-scale cell culture processes

Rahmatnejad

Tolosa²,

Ge³

et al. 2022

Front. Bioeng. Biotechnol.

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Disposable small-scale vessels are commonly used in cell culture studies in academia as well as early stages of bioprocess development. These types of research are crucial for our understanding about cells and bioprocesses as they provide important information regarding different parameters affecting cells. Dissolved carbon dioxide (DCO2) is one main parameter affecting cell metabolism. It is also an indicator of cell culture well-being. Despite CO2 being a critical process parameter, there is a lack of appropriate monitoring system for CO2 in small-scale vessels. Here, we present a membrane-based noninvasive method for measuring DCO2 in cell culture medium. The idea was achieved by modifying a T-flask and replacing a small area of it with CO2 permeable silicone membrane. In the proposed method, the concentration of CO2 dissolved in the cell culture medium is determined by measuring the initial diffusion rate of CO2 through a silicone membrane attached to the bottom wall of the T-flask. The measurement method was validated previously, and the efficacy of the noninvasive method was evaluated by growing E.coli, Pichia pastoris, and CHO cells in the proposed prototype. The results obtained from this method were verified with other quantitative data obtained from the process such as optical density (OD), cell density, dissolved oxygen (DO) and pH. The results show that the proposed membrane-based method is an effective way for completely noninvasive monitoring of DCO2 in small-scale cell culture processes. Additional diffusing species such as oxygen could also be measured using the same approach.

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Anaerobic and Aerobic Respiration in Yeast: Small-Scale Variations on a Classic Laboratory Activity

Cited by 11 publications

References 10 publications

Characterisitcs of Saccharomyces boulardii for reducing ammonia emission from livestock manure

Characterisitcs of Saccharomyces boulardii for reducing ammonia emission from livestock manure

Magnetization of Yeast by Labeling with Iron Complexes: An Undergraduate Laboratory Experiment

A novel approach to noninvasive monitoring of dissolved carbon dioxide in small-scale cell culture processes

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