Anaerobic and aerobic cleavage of the steroid core ring structure by Steroidobacter denitrificans

Wang, Po-Hsiang; Leu, Yann‐Lii; Ismail, Wael; Tang, Sen-Lin; Tsai, Ching-Yen; Chen, Hsing‐Ju; Kao, Ann-Tee; Chiang, Yin-Ru

doi:10.1194/jlr.m034223

Cited by 50 publications

(61 citation statements)

References 41 publications

(56 reference statements)

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“…denitrificans (Jin et al, 2011). We previously hypothesized that a MhyADHlike molybdoenzyme might catalyze the hydration of 1-testosterone (Wang et al, 2013B). Similar genes were harbored by Stl.…”

Section: Sdomentioning

confidence: 89%

“…denitrificans The androgen metabolite profile (Wang et al, 2013B) and genomic and transcriptomic (this study) analyses suggested the involvement of a MhyADH-like molybdoenzyme in anaerobic testosterone catabolism by Sdo. denitrificans.…”

Section: Sdomentioning

confidence: 99%

“…denitrificans and the identification of genes expressed during anaerobic testosterone degradation Depending on oxygen availability, Sdo. denitrificans degrades testosterone through two distinct pathways (Wang et al, 2013B). Therefore, we proposed that testosterone catabolic genes were regulated on the basis of the prevailing oxygen tension.…”

Section: Sdomentioning

confidence: 99%

“…denitrificans cells suggested the involvement of various redox enzymes in the initial steps of both catabolic pathways (Wang et al, 2013B). In the Sdo.…”

Section: Sdomentioning

confidence: 99%

“…denitrificans DSMZ 18526 (gammaproteobacterium) and Sterolibacterium (Stl.) denitrificans DSMZ 13999 (betaproteobacterium) as model microorganisms (Wang et al, 2013b(Wang et al, , 2014 (Figure 1b). However, information regarding the catabolic genes and enzymes involved in the anaerobic catabolic pathway is scant.…”

Section: Introductionmentioning

confidence: 99%

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Integrated multi-omics analyses reveal the biochemical mechanisms and phylogenetic relevance of anaerobic androgen biodegradation in the environment

et al. 2016

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Steroid hormones, such as androgens, are common surface-water contaminants. However, literature on the ecophysiological relevance of steroid-degrading organisms in the environment, particularly in anoxic ecosystems, is extremely limited. We previously reported that Steroidobacter denitrificans anaerobically degrades androgens through the 2,3-seco pathway. In this study, the genome of Sdo. denitrificans was completely sequenced. Transcriptomic data revealed gene clusters that were distinctly expressed during anaerobic growth on testosterone. We isolated and characterized the bifunctional 1-testosterone hydratase/dehydrogenase, which is essential for anaerobic degradation of steroid A-ring. Because of apparent substrate preference of this molybdoenzyme, corresponding genes, along with the signature metabolites of the 2,3-seco pathway, were used as biomarkers to investigate androgen biodegradation in the largest sewage treatment plant in Taipei, Taiwan. Androgen metabolite analysis indicated that denitrifying bacteria in anoxic sewage use the 2,3-seco pathway to degrade androgens. Metagenomic analysis and PCR-based functional assays showed androgen degradation in anoxic sewage by Thauera spp. through the action of 1-testosterone hydratase/dehydrogenase. Our integrative 'omics' approach can be used for culture-independent investigations of the microbial degradation of structurally complex compounds where isotopelabeled substrates are not easily available.

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Section: Sdomentioning

confidence: 89%

Section: Sdomentioning

confidence: 99%

Section: Sdomentioning

confidence: 99%

“…denitrificans cells suggested the involvement of various redox enzymes in the initial steps of both catabolic pathways (Wang et al, 2013B). In the Sdo.…”

Section: Sdomentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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