Anaerobic and Aerobic Bacteria in Secretions of Prostate and Seminal Vesicles of Infertile Men

Abstract

A cytological and bacteriological study was made of prostatic (EPS) and vesicular (EVS) secretions from 123 infertile men who were suspected of having chronic genital tract inflammation and from 31 men with premature ejaculation (1,12,16). In the microbiological investigations samples were inoculated within 10 min on various culture media and incubated under both aerobic and anaerobic conditions. Bacterial loads of more than 10,000 colony-forming units of a single species or genus per milliliter of EPS or EVS were considered to be pathological. In the infertile subjects with proven inflammation of the seminal accessory glands, the EPS and the EVS that gave positive cultures and had bacterial loads defined as pathological contained large numbers of anaerobic or microaerophilic organisms (EPS: 51 of the 63 bacterial strains found, congruent to 81%; EVS: 19 of the 20 bacterial strains found, congruent to 95%).

Relevance of Leukocytospermia and Semen Culture and Its True Place in Diagnosing and Treating Male Infertility

“…In cases of suspected MAGI or a patient with a positive semen culture, prostatic massage may be performed in order to examine the expressed prostate secretion (EPS). The cytological examination of EPS is often diagnostic and is followed by EPS culture [ 91 92 ]. EPS examination is an easy procedure for urologists or andrologists and may be a “second line” diagnostic tool in suspected cases of MAGI or MGTI.…”

High-Resolution Protein Patterns of Human Expressed Prostatic Secretion: A New Tool for the Diagnosis of Prostatitis

“…The first two drops of prostatic secretion were discarded. One drop was then collected for microbiological investigation with immediate preparation of aerobic and anaerobic cultures [5]. A further drop was used for cytological examination as described by Anderson and Weller [ 11.…”