Anaerobic ammonium oxidation in an estuarine sediment

Risgaard-Petersen, Nils; Meyer, Rikke Louise; Schmid, Markus; Jetten, Mike S. M.; Enrich‐Prast, Alex; Rysgaard, Søren; Revsbech, Niels Peter

doi:10.3354/ame036293

Cited by 243 publications

(190 citation statements)

References 49 publications

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“…Various anoxic marine ecosystems have been monitored for anammox activity and the presence of anammox cells (Kuypers et al 2003Rysgaard et al 2004;Risgaard-Petersen et al 2004;Schmid et al 2007). The anammox bacteria in marine environments seem to be restricted to the Scalindua branch with relatively little biodiversity as reflected in very similar 16S rRNA gene sequences (Fig.…”

Section: The Anammox and Nc10 Bacteriamentioning

confidence: 99%

Potential roles of anaerobic ammonium and methane oxidation in the nitrogen cycle of wetland ecosystems

Zhu

Jetten

Kuschk

et al. 2010

Appl Microbiol Biotechnol

166

108

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Anaerobic ammonium oxidation (anammox) and anaerobic methane oxidation (ANME coupled to denitrification) with nitrite as electron acceptor are two of the most recent discoveries in the microbial nitrogen cycle. Currently the anammox process has been relatively well investigated in a number of natural and man-made ecosystems, while ANME coupled to denitrification has only been observed in a limited number of freshwater ecosystems. The ubiquitous presence of anammox bacteria in marine ecosystems has changed our knowledge of the global nitrogen cycle. Up to 50% of N 2 production in marine sediments and oxygendepleted zones may be attributed to anammox bacteria. However, there are only few indications of anammox in natural and constructed freshwater wetlands. In this paper, the potential role of anammox and denitrifying methanotrophic bacteria in natural and artificial wetlands is discussed in relation to global warming. The focus of the review is to explore and analyze if suitable environmental conditions exist for anammox and denitrifying methanotrophic bacteria in nitrogen-rich freshwater wetlands.

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Section: The Anammox and Nc10 Bacteriamentioning

confidence: 99%

Potential roles of anaerobic ammonium and methane oxidation in the nitrogen cycle of wetland ecosystems

Zhu

Jetten

Kuschk

et al. 2010

Appl Microbiol Biotechnol

166

108

View full text Add to dashboard Cite

show abstract

“…Measuring anammox and denitrification rate with 15 N-labeled ammonium and nitrate The presence, activity and potential of anammox and denitrifying bacteria were measured as described in Risgaard-Petersen et al (2004) and Engström et al (2005). Soil samples of known weight and density were transferred to the He-flushed, 6.6-ml glass vials (Exetainer, Labco, High Wycombe, Buckinghamshire, UK), together with N 2 -purged media water from the paddy field.…”

Section: Methodsmentioning

confidence: 99%

Anaerobic ammonia oxidation in a fertilized paddy soil

et al. 2011

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Evidence for anaerobic ammonium oxidation in a paddy field was obtained in Southern China using an isotope-pairing technique, quantitative PCR assays and 16S rRNA gene clone libraries, along with nutrient profiles of soil cores. A paddy field with a high load of slurry manure as fertilizer was selected for this study and was shown to contain a high amount of ammonium (6.2-178.8 mg kg À1 ). The anaerobic oxidation of ammonium (anammox) rates in this paddy soil ranged between 0.5 and 2.9 nmolN per gram of soil per hour in different depths of the soil core, and the specific cellular anammox activity observed in batch tests ranged from 2.9 to 21 fmol per cell per day. Anammox contributed 4-37% to soil N2 production, the remainder being due to denitrification. The 16S rRNA gene sequences of surface soil were closely related to the anammox bacteria 'Kuenenia', 'Anammoxoglobus' and 'Jettenia'. Most of the anammox 16S rRNA genes retrieved from the deeper soil were affiliated to 'Brocadia'. The retrieval of mainly bacterial amoA sequences in the upper part of the paddy soil indicated that nitrifying bacteria may be the major source of nitrite for anammox bacteria in the cultivated horizon. In the deeper oxygen-limited parts, only archaeal amoA sequences were found, indicating that archaea may produce nitrite in this part of the soil. It is estimated that a total loss of 76 g N m À2 per year is linked to anammox in the paddy field.

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“…Following homogenisation, * 1 g wet sediment and 900 lL of nitrate-free artificial river water (Smart and Barko 1985) were added to gas-tight vials (3 mL). The sediment slurries were then left to ''pre-incubate'' for 24 h to reduce any traces of ambient 14 N-nitrate and oxygen before starting the 15 N experiments (Risgaard-Petersen et al 2004). To start the experiment, 100 lL of 98 at.% 15 N-sodium nitrate was injected through the butyl septum of each vial (n = 4 per original sediment sample), then they were then gently shaken (rpm 60, SSM1 Orbital Shaker, Stuart, Stone, UK) before bacterial activity was stopped via injection of 100 lL of zinc chloride after 0, 1.5, 4 and 6 h (see Lansdown et al 2012 for more detail).…”

Section: Laboratory Determination Of Nitrate Reducing Potentials In Smentioning

confidence: 99%

Enhanced hyporheic exchange flow around woody debris does not increase nitrate reduction in a sandy streambed

et al. 2017

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Anthropogenic nitrogen pollution is a critical problem in freshwaters. Although riverbeds are known to attenuate nitrate, it is not known if large woody debris (LWD) can increase this ecosystem service through enhanced hyporheic exchange and streambed residence time. Over a year, we monitored the surface water and pore water chemistry at 200 points along a * 50 m reach of a lowland sandy stream with three natural LWD structures. We directly injected 15 N-nitrate at 108 locations within the top 1.5 m of the streambed to quantify in situ denitrification, anammox and dissimilatory nitrate reduction to ammonia, which, on average, contributed 85, 10 and 5% of total nitrate reduction, respectively. Total nitrate reducing activity ranged from 0 to 16 lM h -1 and was highest in the top 30 cm of the stream bed. Depth, ambient nitrate and water residence time explained 44% of the observed variation in nitrate reduction; fastest rates were associated with slow flow and shallow depths. In autumn, when the river was in spate, nitrate reduction (in situ and laboratory measures) was enhanced around the LWD compared with non-woody areas, but this was not seen in the spring and summer. Overall, there was no significant effect of LWD on nitrate reduction rates in surrounding streambed sediments, but higher pore water nitrate concentrations and shorter residence times, close to LWD, indicated enhanced delivery of surface water into the streambed under high flow. When hyporheic exchange is too strong, overall nitrate reduction is inhibited due to short flow-paths and associated high oxygen concentrations.

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Anaerobic ammonium oxidation in an estuarine sediment

Cited by 243 publications

References 49 publications

Potential roles of anaerobic ammonium and methane oxidation in the nitrogen cycle of wetland ecosystems

Potential roles of anaerobic ammonium and methane oxidation in the nitrogen cycle of wetland ecosystems

Anaerobic ammonia oxidation in a fertilized paddy soil

Enhanced hyporheic exchange flow around woody debris does not increase nitrate reduction in a sandy streambed

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