Anaerobe Kultivierung von <i>Lactococcus lactis</i> im Festbettreaktor

Pörtner, Ralf; Seemuk, M.; Schlothauer, Ralf-Christian; Elsser, Dieter

doi:10.1002/cite.200407012

Cited by 7 publications

(3 citation statements)

References 15 publications

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“…Fixed-bed bioreactors consist of a mostly cylindrical column containing macroporous carriers, wherein cells are immobilized ( Figure 1A). The column is permanently perfused with fresh medium [4,7]. If required, the medium can be circulated in a loop ( Figure 1B).…”

Section: Fixed-bed Reactor Systems 21 Principlementioning

confidence: 99%

Design and Operation of Fixed-Bed Bioreactors for Immobilized Bacterial Culture

Pörtner¹,

Faschian²

2019

Growing and Handling of Bacterial Cultures

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Fixed-bed processes operated in perfusion, where cells are immobilized within macroporous carriers, are a promising alternative to processes with suspended microbial or mammalian cells. Their potential has been demonstrated for many purposes. Nevertheless, the number of industrial fixed-bed processes is quite small. To some extent, this is due to the lack of process development tools for fixed-bed processes. To fill this gap, a strategy was developed for the design and evaluation of relevant process parameters of fixed-bed processes. A scale-up concept is presented in order to evaluate the performance as part of process design of fixed-bed processes. This comprises fixed-bed reactors on three different scales, the smallest being the downscaled Multiferm with 10 mL fixed-bed units, the second a 100 mL fixed-bed reactor, and the third a pilot-scale reactor with 1 L fixed-bed volume. The performance of this concept will be discussed for fixed-bed cultures of lactic acid bacteria. Furthermore, a reaction kinetic model for the design of fixed-bed reactors will be presented.

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Section: Fixed-bed Reactor Systems 21 Principlementioning

confidence: 99%

Design and Operation of Fixed-Bed Bioreactors for Immobilized Bacterial Culture

Pörtner¹,

Faschian²

2019

Growing and Handling of Bacterial Cultures

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“…nisin) and aromatic compounds under high volumetric productivity compared to suspension cultivation [3, 5, 9 -13]. One reason for this is the application of dilution rates that are several times higher than the maximum specific growth rate during exponential growth [6,12,14]. Nevertheless, the number of industrial fixed-bed processes is quite small because of the lack of process development tools for fixed-bed processes.…”

Section: Introductionmentioning

confidence: 99%

Multi-Fixed-Bed Bioreactor System Applied for Bioprocess Development of Immobilized Lactic Acid Bacteria

Faschian¹,

De²,

Pörtner³

2016

TOBIOTJ

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Abstract:The multi-fixed-bed bioreactor "Multiferm" provides a downscaled system for the development of fixed-bed processes with immobilized microorganisms or cells. It consists of twelve single fixed-bed units that can be operated independently. Different bacterial strains, carriers used for immobilization, media or flow rates can be studied in one compact experimental set-up. Experiments can be run in parallel to allow for determination of experimental errors. In these studies several continuous cultivations were carried out with Lactobacillus delbrueckii ssp. bulgaricus using different dilution rates and culture durations as proof-ofconcept for the Multiferm. Parameters relevant for process design such as reproducibility of simultaneously performed cultures, optimal dilution rate and long-term performance were determined by means of volume specific lactate and biomass productivity. First indications on a reduced growth rate of the cells due to immobilization were found.

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“…One possibility to make these processes more profitable is the use of cheap raw materials [17] and to increase productivity. For the latter, fixed-bed processes, where cells are immobilized within macroporous carriers, are a promising alternative to common processes with suspended cells [18][19][20]. Immobilization of cells on inert porous carriers is an easy way to retain biomass as described in literature for many applications [20][21][22][23][24][25][26].…”

Section: Introductionmentioning

confidence: 99%