Anabolism versus catabolism of [5-3H]uridine and its relationship to ribonucleic acid labelling in mouse liver after partial hepatectomy

Yngner, Torsten; Engelbrecht, Claes; Lewan, Lillemor; Annerfeldt, Jan-Erik

doi:10.1042/bj1780001

Cited by 14 publications

(3 citation statements)

References 41 publications

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“…The radioactivity of UTP was determined in the neutralized acid-soluble fraction after homogenization in 0.4 M HCIO... The amount of UTP used for calculation of the UTP-specific radioactivity was 257 nmol/g wet weight (33). Specific radioactivity of RNA was determined in the precipitated acid-insoluble material obtained after homogeniza tion of the liver tissue in the 0. by others (5).…”

Section: Resultsmentioning

confidence: 99%

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Possible Significance of Changes in the Energy Metabolism for the Release of Liver Lactate Dehydrogenase and for the Uptake and Incorporation of [<sup>14</sup>C]-Orotic Acid into Liver Ribonucleic Acid after Partial Hepatectomy

Yngner¹,

Bengtsson²,

Carlberg³

et al. 1980

Pathobiology

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The mouse liver revealed no increased incorporation of [¹⁴C]-orotic acid into either the total acid-soluble fraction, the uridine triphosphate or the RNA at 6 and 24 h after partial hepatectomy. In regenerating mouse and rat liver, the concentration of adenosine triphosphate was decreased 15–20% at 6 h, but was in the same range as that of the controls at 24 h. The adenosine monophosphate concentration of mouse liver increased 4-fold and 2-fold at these times after partial hepatectomy, respectively. The results indicate no direct relationship between the energy metabolism and the uptake and incorporation of orotic acid into RNA of regenerating liver. The activity of mouse plasma lactate dehydrogenase 5 (LDH 5) was increased 12-fold at 6 h and 5-fold at 24 h after partial hepatectomy. In rat, the LDH 5 activity was increased 2-fold at 6 h but was not different from that of the controls at 24 h. An increased leakage of LDH 5, possibly related to the decreased energy content of the liver, was thus revealed by the partially hepatectomized mice.

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Section: Resultsmentioning

confidence: 99%

“…Nor is there an increased incorporation of labelled uridine into mouse liver UTP and RNA at these times (33) or at 48 h ( Yngner et al, unpublished results) after partial hepatectomy.…”

Section: Introductionmentioning

confidence: 99%

Possible Significance of Changes in the Energy Metabolism for the Release of Liver Lactate Dehydrogenase and for the Uptake and Incorporation of [<sup>14</sup>C]-Orotic Acid into Liver Ribonucleic Acid after Partial Hepatectomy

Yngner¹,

Bengtsson²,

Carlberg³

et al. 1980

Pathobiology

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“…In contrast with regenerating rat liver, mouse liver reveals no enhanced uptake of orotic acid or incorporation of this precursor into either UTP or RNA at 6 and 24h after partial hepatectomy (Yngner et al, 1979a(Yngner et al, , 1980, nor is there an increased incorporation of labelled uridine into UTP or RNA of regenerating mouse liver, despite an increased uptake of the precursor into the total acid-soluble fraction (Yngner et al, 1979b).…”

mentioning

confidence: 90%

RNA synthesis in regenerating mouse liver evaluated by incorporation of [methyl-14C]methionine and by determination of RNA polymerase activity

Ljungquist¹,

Yngner²,

Lewan³

et al. 1984

Biochemical Journal

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The synthesis of RNA during mouse liver regeneration was studied by two different methods at 24 and 48 h after partial hepatectomy. Total chromatin-bound RNA polymerase activity showed an increase of 32% at 24 h after partial hepatectomy. At 48 h a slight increase in total activity was also observed in regenerating liver, but the difference was not significant. The increase in total RNA polymerase activity was due to a rise in RNA polymerase I plus III activity. This enzyme activity was increased at both 24 and 48 h. The increase was 57% at 24 h and 51% at 48 h. When [methyl-14C]methionine was used for labelling of methyl groups in rRNA, there was an increased specific radioactivity of this class of RNA at both 24 h and 48 h. The increases were 263 and 103% at 24 and 48 h respectively. Thus both methods revealed an increased synthesis of rRNA during mouse liver regeneration. The results are discussed in relation to previous results from this laboratory [Yngner, Carlberg, Lewan & Engelbrecht (1979) Hoppe-Seyler's Z. Physiol. Chem. 360, 1069-1074; Yngner, Engelbrecht, Lewan & Annerfeldt (1979) Biochem. J. 178, 1-8; Yngner, Bengtsson, Carlberg, Engelbrecht & Wieslander (1980) Exp. Cell. Biol. 48, 393-403], which have shown that the incorporation of orotic acid or uridine into RNA is not increased in mouse liver regenerating after partial hepatectomy.

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Capacity of rat liver for pyrimidine synthesis and catabolism during fetal and neonatal development

Hardiman

Alfant

Wakelin

et al. 1983

Archives of Biochemistry and Biophysics

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Anabolism versus catabolism of [5-3H]uridine and its relationship to ribonucleic acid labelling in mouse liver after partial hepatectomy

Cited by 14 publications

References 41 publications

Possible Significance of Changes in the Energy Metabolism for the Release of Liver Lactate Dehydrogenase and for the Uptake and Incorporation of [<sup>14</sup>C]-Orotic Acid into Liver Ribonucleic Acid after Partial Hepatectomy

Possible Significance of Changes in the Energy Metabolism for the Release of Liver Lactate Dehydrogenase and for the Uptake and Incorporation of [<sup>14</sup>C]-Orotic Acid into Liver Ribonucleic Acid after Partial Hepatectomy

RNA synthesis in regenerating mouse liver evaluated by incorporation of [methyl-14C]methionine and by determination of RNA polymerase activity

Capacity of rat liver for pyrimidine synthesis and catabolism during fetal and neonatal development

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