Anabolic Response of Mouse Bone-Marrow-Derived Stromal Cell Clone ST2 Cells to Low-Intensity Pulsed Ultrasound

Naruse, Keiko; Mikuni-Takagaki, Yuko; Azuma, Yoshiaki; Ito, Masaya; Oota, Tomohiro; Kameyama, Kohzoh; Itoman, Moritoshi

doi:10.1006/bbrc.2000.2094

Cited by 119 publications

(92 citation statements)

References 23 publications

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“…Moreover, high cellularity and less mineralization at the early stage of consolidation might be in favor of LIPUS treatment. Periosteal cells, 32 marrow stem cells, 33 and less mature osteoblasts, 34 which were found in distraction callus, were stimulated by LIPUS in vitro studies.…”

Section: Discussionmentioning

confidence: 95%

Low intensity pulsed ultrasound accelerated bone remodeling during consolidation stage of distraction osteogenesis

Chan

Qin

Lee

et al. 2005

Journal Orthopaedic Research

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Bone regeneration in distraction osteogenesis occurs under tensile stress with axial rhythmic distraction after osteotomy. In this study, we evaluated if the low intensity pulsed ultrasound (LIPUS) was also effective on enhancement of bone remodeling during consolidation stage of distraction osteogenesis. Open osteotomy of seventeen 18-week-old female New Zealand rabbit tibiae were performed. The distraction was applied with the rate of 1 mm per day. LIPUS (30 mW/cm 2 , 1.5 MHz) was delivered for 20 min per day during 4-week consolidation stage (n ¼ 10). The animals without treatment served as sham group (n ¼ 7). Plain X-ray, peripheral quantitative computational tomography (pQCT), and torsional test were performed. Results showed that smaller radiolucent interzone of LIPUS treatment group was gradually occupied by calcified tissue in plain X-ray at week 2. The bone mineral density (BMD) measured on radiographs increased by 9.18% in the LIPUS group. Bone mineral content (BMC), hard callus volume, and bone strength index (BSI) measured by pQCT were 83%, 116%, and 94%, respectively, in LIPUS group that were significantly greater than those of the controls. At the 4th week, LIPUS-treated callus showed the development of neocorticalization in the proximal and distal region. The BMC, hard callus volume, and BSI of LIPUS group decreased and was not significantly different from control. This was also confirmed by the maximum torque of LIPUS-treated callus (1424.2 AE 457.3 N Á mm) obtained at week 4, which did not differ from that of the sham group (1968.8 AE 895.1 N Á mm). In conclusion, the effective period of LIPUS treatment was at the initial stage of consolidation, with accelerated bone formation and remodeling. ß

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Section: Discussionmentioning

confidence: 95%

Low intensity pulsed ultrasound accelerated bone remodeling during consolidation stage of distraction osteogenesis

Chan

Qin

Lee

et al. 2005

Journal Orthopaedic Research

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“…66 Low-intensity pulsed ultrasound In vitro and in vivo studies have demonstrated that lowintensity pulsed ultrasound (US) might constitute an osteogenic stimulus. 83,84 Recently, Warden et al 85 applied specific US at the calcaneum for 6 weeks in young subjects with 1-6-month histories of complete SCI. The results showed that low-intensity pulsed US was unable to protect against SCI-induced calcaneal bone demineralization.…”

Section: Physical Exercisementioning

confidence: 99%

Bone loss in spinal cord-injured patients: from physiopathology to therapy

et al. 2005

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Study design: Review article on bone metabolism and therapeutic approach on bone loss in patients with spinal cord injury (SCI). Objective: The first part aims to describe the process of bone demineralization and its effects on bone mass in patients with SCI. The second part describes and discusses the therapeutic approaches to limiting the alteration in bone metabolism related to neurological lesions. Setting: Propara Rehabilitation Center, Montpellier, France. Results: During the first 24 months postinjury, demineralization occurs exclusively in the sublesional areas and predominantly in weight-bearing skeletal sites such as the distal femur and proximal tibia, both of which are trabecular-rich sites. Reduced bone mass, in association with a modified bone matrix property and composition, is very likely at the origin of pathological fractures after minor trauma to which these patients are frequently exposed. Since these fractures may be asymptomatic yet may lead to complications, preventing and managing 'neurological osteoporosis' remains a considerable challenge. Two main approaches are considered: the first consists in applying a mechanical stimulus to the bone tissue by standing, orthotically aided walking or functional electrical stimulation (FES). The second uses medications, particularly antiresorptive drugs such as calcitonin or diphosphonates. Conclusion: To develop well-adapted treatments, a more precise understanding of bone loss etiology is needed. The current rehabilitation programs are based on the idea that the bone physiological changes observed in patients with SCI are due to immobility, but results indicate that alterations inherent to neurological damage may play an even greater role in inducing osteoporosis.

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“…[12][13][14][15] The results of several histological studies have shown that LIPUS has an important influence on key functional activities of all major cell types involved in bone healing, such as osteoblasts, osteoclasts, chondrocytes, and mesenchymal stem cells. [16,17] In addition, several studies showed a positive effect of US to increase the levels of intracellular calcium incorporation in cultures of differentiating bone and cartilage cells. Increases in calcium incorporation were modulated transforming growth factor (TGF)-beta and adenylate cyclase activity.…”

Section: Review Articlementioning

confidence: 99%

“…[40] Other several in vitro studies had shown LIPUS elevated mRNA levels for insulin-like growth factor-I, OC, and BSP and also it was found to stimulate mRNA expression of the bone matrix proteins ALP and OC in UMR-106 cells. [16,17] In addition, there have been some reports that LIPUS may have a direct effect on cell membrane permeability. [23,[45][46][47][48][49][50] …”

Section: Lipus In Cellular Levelsmentioning

confidence: 99%

Untitled

Rashidi

2017

AJP

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Ultrasound (US) waves due to their unique features can be a treatment option for osteoporosis. Initial studies have shown promising but controversial outcomes. Several preclinical and clinical studies have been conducted on osteoporosis and studies are ongoing to find optimum US parameters, mechanisms of action, and therapeutic efficacies of these techniques for osteoporosis treatment. This paper was aimed to review the recent advances of using US waves in the treatment of osteoporosis and possible mechanisms of actions. The databases of PubMed (1980PubMed ( -2016, EMBASE (1980EMBASE ( -2016, Web of Sciences (1980-2016), and Google Scholar (1980-2016 were searched using the set terms. The obtained records were reviewed, and relevant studies were selected for comprehensive review of the current literature. Low-intensity pulsed US (LIPUS) has biological effects on the bone healing process and it can accelerate bone regeneration. Current evidence is limited on the efficacy of US waves for treatment or prevention of osteoporosis; however, the initial studies are promising. The US waves can promote osteoblast and inhibit osteoclast, enhance angiogenesis, trigger expression of different genes associated with osteogenesis. No definite dose-response existed on the clinical trials of US wave applications. The current evidence shows the therapeutic efficacy of US waves particularly LIPUS for osteoporosis treatment; however, to observe therapeutic outcomes long-term US stimulation is required. No definitive dose-response is proposed for osteoporosis. Further in vitro and clinical trials should be conducted to develop US-based techniques for the treatment of osteoporosis as a clinical treatment option.

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Anabolic Response of Mouse Bone-Marrow-Derived Stromal Cell Clone ST2 Cells to Low-Intensity Pulsed Ultrasound

Cited by 119 publications

References 23 publications

Low intensity pulsed ultrasound accelerated bone remodeling during consolidation stage of distraction osteogenesis

Low intensity pulsed ultrasound accelerated bone remodeling during consolidation stage of distraction osteogenesis

Bone loss in spinal cord-injured patients: from physiopathology to therapy

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