This study compares the diagnostic of "Tonsillitis" is inflammation of the pharyngeal tonsils and is also known as pharyngitis to extend the inflammation to the adenoid and the lingual tonsils. Most cases of bacterial tonsillitis occur group β-"hemolytic Streptococcus pyogenes" (GABHS), "S. aureus" generally. The current study aims to bacterial profile assessment with serum CRP, antibodies RF, and Anti-CCP in acute & chronic tonsillitis patients in Babylon Province. Completely, (45) a sample of blood and swabs of (22-56) years of male and female tonsillitis patients. Sera antibodies of (CRP, RF, and anti-CCP) identified by serological techniques for every one of these patients. Results showed that the culture of the samples gave all patient 45 positive growths and the presentation of the positive conclusion in grams of positive bacteria and negative grams depending on the staining. The highest percentages of S. pyogenes and S. aureus in patients with tonsillitis of other pathogens. Additionally, it is the highest proportion of tonsillitis disease is unlike the healthy individual's group according to different criteria by age group (21-59) years, disease duration (4-13) years, females and autoantibody tests (CRP, RF, and Anti-CCP antibodies) in patients with tonsillitis patients (acute & chronic). Our study concluded that it is important to determine the values of these criteria for their effects on patients suffering from acute and chronic tonsillitis.