An α2,3-Sialyltransferase fromPhotobacteriumsp. JT-ISH-224 TransfersN-Acetylneuraminic Acid to Both the O-2 and O-3′ Hydroxyl Groups of Lactose

Chemical approach for the syntheses of GM4 isomers with sialic acid to non-natural linkage positions on galactose

“…Therefore, a synthetic method that enables the introduction of sialic acid groups at the sites of the poor reactive hydroxyl groups in galactose is required. Recently, Mine et al 14 reported that a2,3-sialyltransferase from Photobacterium sp. JT-ISH-224 produced a regio-mistaken sialyl-transferred sialoside which had sialic acid at the C2 hydroxyl group of lactose.…”

Loss-of-Function Mutation in Bi-Functional Marine Bacterial Sialyltransferase

“…Lactose was from Wako Pure Chemical Industries (Osaka, Japan). 3 0 -Sialyllactose was synthesized enzymatically using marine bacterial 2,3-sialyltransferase, and purified by a method described by Mine et al 33) All other reagents were of commercially available analytical grade.…”

Recent Developments in Enzymatic Synthesis of Modified Sialic Acid Derivatives

“…Both pst3‐224 and pst6‐224 STs belong to the GT80 family with only pst6‐224 having a reported crystal structure 69. Pst3‐224 has unique acceptor specificity (Figure 6) and was reported to transfer Neu5Ac to both the O′ ‐3 hydroxy group and O ‐2 hydroxy group of Lac simultaneously to give 2,3′‐disialyllactose 96. In addition, pst3‐224 transfers Neu5Ac to the β‐anomeric hydroxy groups of mannose (Man) and Manα1,6Man 88.…”