An UPLC-MS/MS Method for Simultaneous Quantification of the Components of Shenyanyihao Oral Solution in Rat Plasma

Abstract

Objectives. To study the quantification of the components in rat plasma after oral administration of Shenyanyihao oral solution. Methods. Shenyanyihao oral solution has been traditionally used for the treatments of chronic nephritis in clinics. Stachydrine, Danshensu, chlorogenic acid, protocatechuic acid, plantamajoside, aesculetin, isoquercitrin, ferulic acid, baicalin, and baicalein are regarded as the main compounds in Shenyanyihao oral solution. A sensitive, efficient, and precise UPLC-MS/MS method was established and validated for the quantification of the components in rat plasma after oral administration of Shenyanyihao oral solution. Results. The main pharmacokinetic parameters of the components were acquired based on the analysis of the plasma sample by a noncompartmental method using the WinNonlin7.0 pharmacokinetic program. Danshensu, protocatechuic acid, isoquercitrin, and ferulic acid from Shenyanyihao oral solution were quickly absorbed, and their peak concentration occurred at less than 0.5 h. The pharmacokinetic parameter of the average t1/2 from Danshensu was 3.91 h in rats, and it was the most rapid distribution and elimination among the components. In addition, the Cmax of stachydrine and baicalin were revealed as the higher plasma concentrations in rats. Conclusions. This pharmacokinetic study seems to be useful for a further clinical study of Shenyanyihao oral solution in the treatments of chronic nephritis.

Pharmacokinetic study of Tangwang Mingmu granule for the management of diabetic retinopathy based on network pharmacology

“…To understand the pharmacokinetic parameters of absorption, distribution, metabolism, and excretion (ADME) of a drug product, the high-content-ingredients that emigrate into the circulatory system are generally selected as target compounds in a pharmacokinetic study (Jiang et al. 2020 ; Liu et al. 2020 ).…”

“…Ten high-content compounds including stachyBine, danshensu, chlorogenic acid, protocatechuic acid, plantamajoside, aesculetin, isoquercitrin, ferulic acid, baicalin, and baicalein were selected for a pharmacokinetic study which resulted in little clinical relevance (Jiang et al. 2020 ). Similarly, Liu et al.…”

“…Pharmacokinetic research plays a critical role in assessing drug clinical dosing parameters, side effects, and treatment mechanisms in vivo (Ding et al 2019;. To understand the pharmacokinetic parameters of absorption, distribution, metabolism, and excretion (ADME) of a drug product, the highcontent-ingredients that emigrate into the circulatory system are generally selected as target compounds in a pharmacokinetic study (Jiang et al 2020;. In certain instances, the activity of the high content ingredients does not match the clinical application of the herbal medicine, resulting in difficulties in obtaining pharmacokinetic indicators to guide clinical use.…”

“…For example, the clinical symptoms of chronic nephritis have been reported to be alleviated by shenyanyihao oral solution. Ten high-content compounds including stachyBine, danshensu, chlorogenic acid, protocatechuic acid, plantamajoside, aesculetin, isoquercitrin, ferulic acid, baicalin, and baicalein were selected for a pharmacokinetic study which resulted in little clinical relevance (Jiang et al 2020). Similarly, reported that Chaihu-Shugan-San could be used to treat depression.…”

Qualitative and quantitative determination of the primary active components and metabolites in human plasma after oral administration of Shuanghuanglian liquid

“…Several LC-MS/MS methods for the simultaneous quantification of baicalin and baicalein in biological samples have been reported [7][8][9][10][11][12][13][14]. However, most of these methods require complex sample preparation to improve detection sensitivity, such as solid-phase extraction, liquid-liquid extraction, or concentration by evaporation.…”