An updated phylogeny ofDeprea(Solanaceae) with a new species from Colombia: interspecific relationships, conservation assessment, and a key for Colombian species

Abstract

Deprea is a neotropical genus that comprises 51 species. Recent work based on molecular data has explored its monophyly and interspecific relationships, but the relationships amongst Deprea species were not entirely elucidated. The inclusion of more accessions and molecular markers in phylogenetic analysis is likely to produce more supported hypotheses. Therefore, the main objective of this study was to perform a combined phylogenetic analysis of Deprea including seven new samples representing six species and one more DNA plastid marker than in previous studies. In that process, a new species of Deprea from Colombia was discovered and is described and illustrated here. Deprea teresitae Deanna & A. Orejuela, sp. nov. is morphologically similar to D. dilloniana and phylogenetically closely related to D. hawkesii and D. harlingiana. This new species is distinguished by the combination of short calyx lobes, non-mucronate anthers, long staminal filaments and corolla lobes, and elongated appressed fruiting calyx around a subglobose green berry. The new phylogenetic results are presented, including discussion on phylogenetic interspecific relationships, taxonomy, geographic distribution, and conservation status from D. teresitae. The synonymy of Deprea sylvarum subsp. novogranatensis under D. glabra is also proposed and an identification key to the 10 Deprea species distributed in Colombia is included.

Repeated evolution of a morphological novelty: a phylogenetic analysis of the inflated fruiting calyx in the Physalideae tribe (Solanaceae)

“…In a prescient review, Hepper () pointed out that these two western African species, W. aristata and W. frutescens , are morphologically unlike others in Withania and suggested that their closest relatives may instead be across the Atlantic. Beyond the rearrangement of Withaninae necessitated by this apparent split within Withania , most of the genera that have been placed in the subtribe are monophyletic (or nearly so), based on extensive taxonomic work in recent years (Zamberlan et al., ; Deanna et al., ).…”

“…Taxon coverage was 92.8% for ITS, 77.9% for LFY , 78.4% for waxy , and 87% for the chloroplast fragment (Appendix S3). DNA extractions were done following a modified 2 × CTAB procedure (Doyle and Doyle, ); primers and polymerase chain reaction conditions followed previous works (Smith and Baum, ; Deanna et al., ).…”

Repeated evolution of a morphological novelty: a phylogenetic analysis of the inflated fruiting calyx in the Physalideae tribe (Solanaceae)

“…In a prescient review, Hepper (1991) pointed out that these two western African species, W. aristata and W. frutescens , are morphologically unlike others in Withania and suggested that their closest relatives may instead be across the Atlantic. Beyond the rearrangement of Withaninae necessitated by this apparent split within Withania , most of the genera which have been placed in the subtribe are monophyletic (or nearly so) given extensive taxonomic work in recent years (Zamberlan et al, 2015; Deanna et al, 2018).…”

“…Taxa coverage was 92.8 % for ITS, 77.9 % for LFY, 78.4 % for waxy , and 87 % for the chloroplast fragment (Appendix S3). DNA extractions were done following a modified 2 x CTAB procedure (Doyle and Doyle, 1987); primers and PCR conditions followed previous work (Smith and Baum, 2006; Deanna et al, 2018).…”

“…given its phylogenetic position outside of Physalideae. However, we included sequences of a voucher previously identified as C. harlingiana (Smith and Baum, 2006; Deanna et al, 2017, 2018), which does fall within Cuatresia and appears to belong to an undescribed taxon (appearing here as Cuatresia sp. ).…”