An Updated Gene Regulatory Network reconstruction of multidrug-resistant Pseudomonas aeruginosa CCBH4851

Abstract

BACKGROUNDHealthcare-associated infections due to multidrug-resistant (MDR) bacteria such as Pseudomonas aeruginosa are significant public health issues worldwide. A system biology approach can help understand bacterial behavior and provide novel ways to identify potential therapeutic targets and the development of new drugs. Gene regulatory networks (GRN) are an example of interaction representation in silico between regulatory genes and their targets.OBJECTIVESIn this work, we update the reconstruction of the MDR P. aeruginosa CCBH4851 GRN, and analyze and discuss its structural properties.METHODSWe based this study on the gene orthology inference methodology using the reciprocal best hit method. The P. aeruginosa CCBH4851 genome and GRN, published in 2019, and the P. aeruginosa PAO1 GRN, published in 2020, were used for this update reconstruction process.FINDINGSOur result is a GRN with a larger number of regulatory genes, target genes, and interactions compared to the previous networks, and its structural properties are consistent with the complexity of biological networks and the biological features of P. aeruginosa.MAIN CONCLUSIONSHere, we present the largest and most complete version of P. aeruginosa GRN published to this date, to the best of our knowledge.