An Update on the Streptococcus bovis Group: Classification, Identification, and Disease Associations

Dekker, John P.; Lau, Anna F.

doi:10.1128/jcm.02977-15

Cited by 80 publications

(77 citation statements)

References 37 publications

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“…1 GSP active form is a 21-mer peptide 2 We first aimed at determining the active form of GSP in Sgg strain UCN34. GSP is derived from 3 a 45 residue precursor encoded by gsp gene, and is predicted to be processed to a 24-mer peptide 4 by cleavage of the amino terminal leader sequence after a double glycine motif, then exported to 5 the extracellular environment by the BlpT transporter.…”

Section: Resultsmentioning

confidence: 99%

Secretion, maturation and activity of a quorum-sensing peptide (GSP) inducing bacteriocins transcription in Streptococcus gallolyticus

Harrington

Proutière

Tal‐Gan

et al. 2020

Preprint

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words)Streptococcus gallolyticus subsp. gallolyticus (Sgg) is an emerging opportunistic pathogen responsible for septicemia and endocarditis in the elderly. Invasive infections by Sgg are strongly linked to the occurrence of colorectal cancer (CRC). It was previously shown that increased secondary bile salts in CRC-conditions enhances the bactericidal activity of gallocin, a bacteriocin produced by Sgg, enabling it to colonize the mouse colon by outcompeting resident enterococci.In a separate study, we have shown that Sgg produces and secretes a 21-mer peptide that activates bacteriocin production. This peptide was named CSP because of its sequence similarity with competence stimulating peptides found in other streptococci. Here we demonstrate that CSP is a bona fide quorum-sensing peptide involved in activation of gallocin gene transcription. We therefore refer to CSP as GSP (gallocin stimulating peptide). GSP displays some unique features since its N-terminal amino-acid lies three residues after the double glycine leader sequence. Herein, we set out to investigate the processing and export pathway that leads to mature GSP. We also conducted the first comprehensive structure-activity relationship (SAR) of Sgg GSP to identify its key structural features.Significance (116 words) Streptococcus gallolyticus subsp. gallolyticus (Sgg) is an opportunistic pathogen associated with colorectal cancer (CRC) and endocarditis. Sgg utilizes quorum-sensing (QS) to regulate the production of a bacteriocin (gallocin) and gain selective advantage in colonizing the colon. In this manuscript, we report 1) the first structure-activty relationship study of the Sgg QS pheromone that regulates gallocin production; 2) evidence that the active QS pheromone is processed to its mature form by a unique ABC transporter and not processed by an extracellular protease; and 3) supporting evidence of interspecies interactions between streptococci pheromones. Our results revealed the minimal pheromone scaffold needed for gallocin activation and uncovered unique interactions between two streptococci species QS signals that warrant further studies.

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Section: Resultsmentioning

confidence: 99%

Secretion, maturation and activity of a quorum-sensing peptide (GSP) inducing bacteriocins transcription in Streptococcus gallolyticus

Harrington

Proutière

Tal‐Gan

et al. 2020

Preprint

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“…The association between S. bovis bacteremia and colonic pathologies is thought to be bacterial translocation from the gut mucosa into blood stream due to the impairment of the gut–vascular barrier integrity . Well‐studied and efficacious treatments include β‐lactams (such as penicillin G, amoxicillin‐clavulanate, and ceftriaxone) and vancomycin for a 2–4‐week course …”

Section: Discussionmentioning

confidence: 99%

Mesenteric ischemia as an unusual cause of Streptococcus bovis bacteremia in the absence of endocarditis or colorectal neoplasm

Tse

Nguyen

2018

JGH Open

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Streptococcus bovis bacteremia is nearly always pathological and has been associated with colorectal cancer and endocarditis. Here, we present the first published case of S. bovis bacteremia secondary to acute mesenteric ischemia. This case illustrates the typical presentation of acute mesenteric ischemia with an atypical consequence of S. bovis bacteremia, as well as the diagnostic evaluations and management options.

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“…To date, the species belonging to the Streptococcus bovis group constitute the non-enterococcal group D streptococci (Dekker and Lau, 2016) .f Facultad de Veterinaria; Universidad Complutense de Madrid; Madrid (Spain) .…”

Section: Methodsmentioning

confidence: 99%

“…To date, the species belonging to the Streptococcus bovis group constitute the non-enterococcal group D streptococci (Dekker and Lau, 2016) .…”

Section: Methodsmentioning

confidence: 99%

Fluorescence Imaging of Streptococcus pneumoniae with the Helix pomatia agglutinin (HPA) As a Potential, Rapid Diagnostic Tool

Domenech

Garcı́a

2017

Front. Microbiol.

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Streptococcus pneumoniae is a common human pathogen and a major causal agent of life-threatening infections that can either be respiratory or non-respiratory. It is well known that the Helix pomatia (edible snail) agglutinin (HPA) lectin shows specificity for terminal αGalNAc residues present, among other locations, in the Forssman pentasaccharide (αGalNAc1→3βGalNAc1→3αGal1→4βGal1→4βGlc). Based on experiments involving choline-independent mutants and different growth conditions, we propose here that HPA recognizes the αGalNAc terminal residues of the cell wall teichoic and lipoteichoic acids of S. pneumoniae. In addition, experimental evidence showing that pneumococci can be specifically labeled with HPA when growing as planktonic cultures as well as in mixed biofilms of S. pneumoniae and Haemophilus influenzae has been obtained. It should be underlined that pneumococci were HPA-labeled despite of the presence of a capsule. Although some non-pneumococcal species also bind the agglutinin, HPA-binding combined with fluorescence microscopy constitutes a suitable tool for identifying S. pneumoniae and, if used in conjunction with Gram staining and/or other suitable technique like antigen detection, it may potentially facilitate a fast and accurate diagnosis of pneumococcal infections.

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An Update on the Streptococcus bovis Group: Classification, Identification, and Disease Associations

Cited by 80 publications

References 37 publications

Secretion, maturation and activity of a quorum-sensing peptide (GSP) inducing bacteriocins transcription in Streptococcus gallolyticus

Secretion, maturation and activity of a quorum-sensing peptide (GSP) inducing bacteriocins transcription in Streptococcus gallolyticus

Mesenteric ischemia as an unusual cause of Streptococcus bovis bacteremia in the absence of endocarditis or colorectal neoplasm

Fluorescence Imaging of Streptococcus pneumoniae with the Helix pomatia agglutinin (HPA) As a Potential, Rapid Diagnostic Tool

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