An update on the roles of circular RNAs in spinal cord injury

Ma, Xuexiao; Wang, Xuesong; Zhang, Xiugong; Gong, Xiaojin; Sun, Ruifu; Wong, Sunny H.; Chan, Matthew T. V.; Wu, William Ka Kei

doi:10.1007/s12035-021-02721-2

Cited by 8 publications

(6 citation statements)

References 38 publications

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“…Previous found that circRNA play a role in the pathological progression after spinal cord injury [17,29,30]. In order to identi eld that if circRNA play a role in HExo mediated spinal cord repair.…”

Section: Discussionmentioning

confidence: 99%

Hypoxic pretreatment ADSCs exosome treatment promote spinal cord repair after spinal cord injury by delivery circ-Astn1 and activation microglia autophagy

Shao

Jin

Zhu

et al. 2022

Preprint

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Background: Exosome (Exo) secretion by hypoxic pretreatment adipose-derived mesenchymal stem cells (ADSCs) promotes spinal cord repair after spinal cord injury (SCI). But the mechanism remains unclear. Methods: High-throughput sequencing was used to investigate abnormal expression of circRNA in hypoxic pretreatment ADSCs exosome (HExo) and ADSCs exosome (Exo). The abnormal expression of mRNA in spinal cord tissues after Exo or HExo treatment were also analysis using high-throughput sequencing. Bioinformatics analysis and luciferase reporting analysis were used to clarify the interacted relationship among circRNA, miRNA and mRNA. BV2 cells were employ to analysis the apoptosis, inflammatory cytokines expression under OGD condition by using immunofluorescence, ELISA detection. SCI mice model were constructed and the therapeutic effect of Exo were detected using immunohistochemistry, immunofluorescence. Results: The result show that HExo have more therapeutic effect on spinal cord repair after SCI by activation of autophagy. High-throughput sequencing detection show that circ-Astn1 play a role in HExo mediated spinal cord repair after SCI. Downregulation circ-Astn1 decreased the therapeutic effect of HExo to spinal cord repair after SCI. The study also found that Atg7 play a role in HExo mediated spinal cord repair after SCI. Luciferase reporting analysis confirmed that both miR-138-5p and Atg7 were downstream targets of circ-Astn1. Downregulation of Atg7 or overexpression of miR-138-5p reversed the protective effect of circ-Astn1 to BV2 after exposure to OGD condition. Upregulation circ-Astn1 increased the therapeutic effect of Exo to spinal cord repair after SCI by activation autophagy. Conclusions: In conclusion, ADSC-Exos containing circ-Astn1 promotes spinal cord repair after SCI by targeting miR-138-5p/Atg7 pathway mediated autophagy.

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Section: Discussionmentioning

confidence: 99%

Hypoxic pretreatment ADSCs exosome treatment promote spinal cord repair after spinal cord injury by delivery circ-Astn1 and activation microglia autophagy

Shao

Jin

Zhu

et al. 2022

Preprint

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“…More and more evidence shows that circRNAs have 5′ cap and 3′ tail structures, and generate covalent closed-loop structure through reverse splicing ( Bagchi, 2018 ; Wu et al, 2019 ), that is, phagocytic microRNAs by acting as a microRNAs sponge. Competing endogenous RNA (ceRNA) is involved in gene expression and regulating transcription, and interacts with microRNA to play an important biological and regulatory role in the progression of disease ( Ma et al, 2022b ). Especially in SCI it can activate astrocytes, improve neuroinflammatory response and regulate neuronal apoptosis, and participate in the regulation of nerve repair and regeneration.…”

Section: Circrnas and Micrornas In Scimentioning

confidence: 99%

MicroRNAs in spinal cord injury: A narrative review

Zhang

Talifu

et al. 2023

Front. Mol. Neurosci.

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Spinal cord injury (SCI) is a global medical problem with high disability and mortality rates. At present, the diagnosis and treatment of SCI are still lacking. Spinal cord injury has a complex etiology, lack of diagnostic methods, poor treatment effect and other problems, which lead to the difficulty of spinal cord regeneration and repair, and poor functional recovery. Recent studies have shown that gene expression plays an important role in the regulation of SCI repair. MicroRNAs (miRNAs) are non-coding RNA molecules that target mRNA expression in order to silence, translate, or interfere with protein synthesis. Secondary damage, such as oxidative stress, apoptosis, autophagy, and inflammation, occurs after SCI, and differentially expressed miRNAs contribute to these events. This article reviews the pathophysiological mechanism of miRNAs in secondary injury after SCI, focusing on the mechanism of miRNAs in secondary neuroinflammation after SCI, so as to provide new ideas and basis for the clinical diagnosis and treatment of miRNAs in SCI. The mechanisms of miRNAs in neurological diseases may also make them potential biomarkers and therapeutic targets for spinal cord injuries.

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“…Globally, there are over 27 million people living with SCI, with approximately one million new cases each year. Among them, falls and car accidents are the main causes [2]. Recently, the trend globally for SCI is for it to affect younger people, mostly under 30 years of age [3].…”

Section: Introductionmentioning

confidence: 99%

Research Progress of MicroRNAs in Spinal Cord Injury

Deng¹,

Chen²

2023

J. Integr. Neurosci.

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Spinal cord injury is a serious and devastating condition. Recently, research into microRNAs (miRNAs) has become increasingly exhaustive and it has been determined that they are closely related to the pathophysiological processes of spinal cord injury. They participate in the regulation of the inflammatory response of spinal cord injury, the death of neuronal cells, and the repair of neural functions, which are related to the recovery of spinal cord injury. This review focuses on the relationship between miRNA and spinal cord injury, lists miRNA-324-5p, miRNA-221 and miRNA-124, which are helpful for the repair of spinal cord injury, and finally summarizes the current research progress of miRNA-based therapies, so as to provide a foundational reference for clinical and scientific researchers.

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An update on the roles of circular RNAs in spinal cord injury

Cited by 8 publications

References 38 publications

Hypoxic pretreatment ADSCs exosome treatment promote spinal cord repair after spinal cord injury by delivery circ-Astn1 and activation microglia autophagy

Hypoxic pretreatment ADSCs exosome treatment promote spinal cord repair after spinal cord injury by delivery circ-Astn1 and activation microglia autophagy

MicroRNAs in spinal cord injury: A narrative review

Research Progress of MicroRNAs in Spinal Cord Injury

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