An Update on Applications of Cattle Mesenchymal Stromal Cells

Merlo, Barbara; Gugole, Penelope Maria; Iacono, Eleonora

doi:10.3390/ani12151956

Cited by 3 publications

(4 citation statements)

References 128 publications

(154 reference statements)

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“…The isolation, in vitro differentiation and immunophenotype of bovine MSCs has already been reported (Table 1). As shown in Table 1, it is clear that bovine MSCs represent a heterogeneous cell population with differences originating from donor age, tissue of origin, genetic background, passage, and culture conditions [10, 51, 52]. For example, Kato et al showed that bone marrow (BM)‐derived bovine MSCs lacked CD90 expression, while other researchers showed a variable expression of CD90 (Table 1) [53].…”

Section: Introductionmentioning

confidence: 99%

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Validation of multiparametric panels for bovine mesenchymal stromal cell phenotyping

et al. 2023

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Bovine mesenchymal stromal cells (MSCs) display important features that render them valuable for cell therapy and tissue engineering strategies, such as self‐renewal, multi‐lineage differentiation, as well as immunomodulatory properties. These cells are also promising candidates to produce cultured meat. For all these applications, it is imperative to unequivocally identify this cell population. The isolation and in vitro tri‐lineage differentiation of bovine MSCs is already described, but data on their immunophenotypic characterization is not yet complete. The currently limited availability of monoclonal antibodies (mAbs) specific for bovine MSC markers strongly hampers this research. Following the minimal criteria defined for human MSCs, bovine MSCs should express CD73, CD90, and CD105 and lack expression of CD14 or CD11b, CD34, CD45, CD79α, or CD19, and MHC‐II. Additional surface proteins which have been reported to be expressed include CD29, CD44, and CD106. In this study, we aimed to immunophenotype bovine adipose tissue (AT)‐derived MSCs using multi‐color flow cytometry. To this end, 13 commercial Abs were screened for recognizing bovine epitopes using the appropriate positive controls. Using flow cytometry and immunofluorescence microscopy, cross‐reactivity was confirmed for CD34, CD73, CD79α, and CD90. Unfortunately, none of the evaluated CD105 and CD106 Abs cross‐reacted with bovine cells. Subsequently, AT‐derived bovine MSCs were characterized using multi‐color flow cytometry based on their expression of nine markers. Bovine MSCs clearly expressed CD29 and CD44, and lacked expression of CD14, CD45, CD73, CD79α, and MHCII, while a variable expression was observed for CD34 and CD90. In addition, the mRNA transcription level of different markers was analyzed using reverse transcription quantitative polymerase chain reaction. Using these panels, bovine MSCs can be properly immunophenotyped which allows a better characterization of this heterogenous cell population.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Validation of multiparametric panels for bovine mesenchymal stromal cell phenotyping

et al. 2023

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“…Bovine MSCs isolated from different adult and fetal tissues have been shown to be immunophenotypically positive for CD29, CD44, CD73, CD90, CD105, CD166, CD49d, CD13 and MHC-I antigens and negative for CD45, CD34, CD14, CD31, CD117, CD74, CD11b, CD80, CD86, CD106 and MHC-II surface markers (Merlo et al, 2022;Wang et al, 2022, Cahuascanco et al, 2019.…”

Section: Immunophenotypic Cytobiochemical Cytophysiological and Molec...mentioning

confidence: 99%

Multi-faceted characterization of mesenchymal stem cells and their application as a powerful tool for agrobiotechnology, assisted reproductive technologies, and veterinary and regenerative biomedicine – a review

Romanek

Trzcińska

Samiec

2024

Annals of Animal Science

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A broad spectrum of cytologically specific immunophenotypic, biochemical, physiological and molecular attributes of neonatal and adult mesenchymal stem cells (MSCs) exists. Taking into account the biological traits of these types of multipotent stem cells, they play a pivotal role in the wide utilization of MSC-mediated engineering and MSC-based assisted reproductive technologies (ARTs) for animal production, veterinary research, regenerative and reconstructive medicine, and cell/tissue transplantology. Consequently, this paper sought to comprehensively provide research highlights, and insightful interpretation of current goals and future targets related to the agricultural, embryological and biomedical application of MSC-assisted biotechnological strategies and MSC-dependent interdisciplinary research techniques.

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“…Nowadays, in veterinary medicine, the owners require their animals to be treated with sophisticated and new treatments with the aim to improve the patient’s life quality but also, in the case of livestock animals, to improve the quality of products, aiming to preserve human health [ 1 ]. MSCs therapy could be then considered as an opportunity for researchers, veterinary practitioners, and animal owners for contributing to animal and human health and well-being.…”

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confidence: 99%