An unusual variant glycophorin expressing protease‐resistant M antigen encoded by the <i>GYPB‐E(2‐4)‐B</i> hybrid gene

Tsuneyama, Hatsue; Isa, Kaoru; Watanabe‐Okochi, Naoko; Ogasawara, Kenichi; Uchikawa, Makoto; Satake, Masahiro

doi:10.1111/vox.12918

Cited by 3 publications

(4 citation statements)

References 10 publications

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“…MNS is a highly polymorphic blood group comprising 50 antigens recognized by International Society of Blood Transfusion by June 2021, some of which may have been generated by genomic recombination among the closely linked genes GYPA , GYPB , and GYPE ( 13 ). In the blood group system of MNS, anti-M antibody is the most common accidental antibody, and immune stimulation is an important factor for the generation of anti-M antibody.…”

Section: Discussionmentioning

confidence: 99%

Analysis of anti-M antibody status and blood transfusion strategy in Hunan, China

Wang¹,

Wang²,

Mo³

et al. 2022

Ann Transl Med

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Background: By analyzing the detection rate of anti-M antibody in patients with the MNS blood group system in the Hunan area, we aimed to explore its clinical significance and blood transfusion strategy. Methods:We retrospectively analyzed the clinical data of patients who had been confirmed to contain anti-M antibodies through serological methods such as the saline tube method and cassette anti-human globulin method.Results: Irregular antibody screening tests had been applied to 94,452 patients, from which 652 results were positive. Among those positive patients, 93 cases were positive for anti-M antibodies, accounting for 14.26% of the positive rate of irregular antibodies; 11 cases had a blood transfusion history, accounting for 11.8%; 59 cases had a pregnancy history, accounting for 63.4%; and 2 cases had a transplant history, accounting for 2.2%. The patients with anti-M antibodies included 23 pregnant woman, accounting for 24.7%, and 19 tumor patients, accounting for 20.4%. A total of 66 cases were immunoglobulin M (IgM) + immunoglobulin G (IgG) class, accounting for 71.0%, 26 cases were IgM class, accounting for 28.0%, and 1 case was IgG class, accounting for 1.0%. Conclusions:The detection rates of anti-M antibody in the Hunan area and unexpected antibodies in literature reports are mainly related to a pregnancy history, and the type of antibody is predominantly IgM + IgG class. The clinical significance of anti-M antibody cannot be ignored, and three media should be used for cross-matching of blood wherever possible to ensure the safety of blood transfusion.

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Section: Discussionmentioning

confidence: 99%

Analysis of anti-M antibody status and blood transfusion strategy in Hunan, China

Wang¹,

Wang²,

Mo³

et al. 2022

Ann Transl Med

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“…Information on the primers and PCR conditions are shown in Tables S1–S3. Exons 1–4 of the GYPA were amplified as described previously 9 …”

Section: Methodsmentioning

confidence: 99%

“…Exons 1-4 of the GYPA were amplified as described previously. 9 The amplified products were sequenced using BigDye Terminator v1.1 Cycle Sequencing kit (Applied Biosystems, Carlsbad, CA) and Genetic Analyzer (Model 3130xl; Applied Biosystems).…”

Section: Analysis Of Gypa and Gyp(b-a) Hybrid Genesmentioning

confidence: 99%

GP.MOT: A novel glycophorin variant identified in a Japanese blood donor

et al. 2021

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Background: In this study, we identified a novel glycophorin variant (GP.MOT) in a Mi a -positive Japanese blood donor. The proband with this glycophorin variant was discovered by antigen screening of samples from 475,493 Japanese blood donors using monoclonal anti-Mi a . Study design and methods: Standard serological techniques and flow cytometry were performed. GP.MOT RBCs were examined by immunoblotting using anti-GPA, anti-MUT or anti-Mur. Genome DNA was extracted from whole blood, and the GYPA/GYPB was analyzed by polymerase chain reactions and Sanger sequencing. Results: The MNS blood group of the proband was M + N + w S-s + with the presence of other low-frequency antigens including Mi a , Mur, MUT, and KIPP. A 43-kDa molecule, which is almost equivalent in size to glycophorin A (GPA), was identified by immunoblotting using monoclonal anti-MUT and anti-Mur. Sanger sequencing clearly indicated that the proband had two different GYPA*M alleles at SNP rs62334651 (GYPA*M232 + 55A and GYPA*M232 + 55G), as well as a GYP(B-A) hybrid allele (GYP*MOT) with breakpoints located on pseudoexon 3 of GYPB from c.210 to c.219. Discussion: We identified a hybrid glycophorin GP.MOT with the deduced unique amino acid sequence GPB (20-45)-GPΨ B (46-70)-GPA (71-149), which has not been previously reported.

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“…Immunoblot analysis of RBC membrane proteins was performed according to the method described previously [20] using monoclonal anti-M (CBC-3; 2B-23/4C9) [21] or anti-N (CBC-14, in-house). GP.Vw (Mi.I)…”

Section: Immunoblottingmentioning

confidence: 99%

Frequencies of glycophorin variants and alloantibodies against Hil and MINY antigens in Japanese

et al. 2021

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Background and Objectives: Antigens of the MNS blood group system are expressed on the red blood cell (RBC) membrane on glycophorin A (GPA) and glycophorin B (GPB) or on hybrid molecules of GPA and GPB. This study investigated the distribution of glycophorin variants and alloantibodies against Hil and MINY among Japanese individuals.Methods: Mi(a+) or Hil+ RBCs were screened using an automated blood grouping machine (PK7300) with monoclonal anti-Mi a or polyclonal anti-Hil. Glycophorin variants were defined by serology with monoclonal antibodies against Mi a , Vw, MUT and Mur, and polyclonal antibodies against Hil, MINY and Hop + Nob (KIPP). The glycophorin variants were further confirmed by immunoblotting and Sanger sequencing. Alloanti-Hil and alloanti-MINY in the plasma were screened using GP.Hil RBCs in an antiglobulin test. The specificity of anti-Hil or anti-MINY was assessed using GP.Hil (Hil+MINY+) and GP.JL (HilMINY+) RBCs.

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An unusual variant glycophorin expressing protease‐resistant M antigen encoded by the GYPB‐E(2‐4)‐B hybrid gene

Cited by 3 publications

References 10 publications

Analysis of anti-M antibody status and blood transfusion strategy in Hunan, China

Analysis of anti-M antibody status and blood transfusion strategy in Hunan, China

GP.MOT: A novel glycophorin variant identified in a Japanese blood donor

Frequencies of glycophorin variants and alloantibodies against Hil and MINY antigens in Japanese

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