An unusual transmissible agent ? MaTu

Závada, Jan; Závadova, Zuzana

doi:10.1007/bf01314029

Cited by 11 publications

(11 citation statements)

References 16 publications

(28 reference statements)

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“…It has already been proven that lymphocytic choriomeningitis virus can establish long-term non-cytolytic infection in vitro and spread in culture by cell-to-cell contacts (Zavada and Zavadova, 1991). As demonstrated using the LCMV strain MX, during persistent infection, the viral particles are not released to medium, so the uninfected cells could not be infected by the regular virus-receptor interaction.…”

Section: Cell-to-cell Transmissionmentioning

confidence: 94%

Lymphocytic choriomeningitis virus: ways to establish and maintain non-cytolytic persistent infection

Labudová¹,

Pastorek²,

Pastoreková³

2016

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Summary. -Lymphocytic choriomeningitis virus (LCMV) is a prototype virus of the Arenaviridae family that is attracting considerable attention both as an important experimental model system to study acute and persistent viral infections, and as a neglected human pathogen of clinical signifi cance. Notably, LCMV is capable of persisting in an infected host, and escaping the immune system. Here we describe the strategies used by the virus to establish and maintain long-term infection in vitro and/or persistent infection in vivo. We discuss how the viral components (RNA, nucleoprotein, glycoprotein, Z protein) manipulate the host cell machinery to facilitate survival and spread of the virus without disturbing the basal cellular processes. Deep understanding of these strategies is inevitable for the development of approaches towards restricting the virus spread and/or preventing its harmful reactivation. Th is review summarizes the current status in this area and presents ideas emerging from existing data.Keywords: lymphocytic choriomeningitis virus; arenaviruses; persistent infection E-mail: virulama@savba.sk; phone: +421-2-59302439. Abbreviations: ATF6 = activating transcription factor 6; DC = dendritic cells; DG = dystroglycan; ECM = extracellular matrix; eIF-4E/G = eukaryotic translation initiation factor 4E or 4G; eIF-4G = eukaryotic translation initiation factor 4G; GAPDH = glyceraldehyd-3-phosphate dehydrogenase; GP1,2 = glycoprotein 1, 2; GPC = glycoprotein precursor; IRE1 = inositol-requiring protein 1; IRF-3 = IFN regulatory factor 3; PERK = PKR-like ER kinase; PML = promyelocytic leukemia; UPR = unfolded protein response; ZP = Z protein

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Section: Cell-to-cell Transmissionmentioning

confidence: 94%

Lymphocytic choriomeningitis virus: ways to establish and maintain non-cytolytic persistent infection

Labudová¹,

Pastorek²,

Pastoreková³

2016

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“…It was identified as the transmembrane carbonic anhydrase isoform IX (CAIX) (Opavsky et al, 1996;Pastorek et al, 1994), the expression of which is predominantly associated with hypoxic tumors of poor prognosis (Wykoff et al, 2000). Previous evidence suggested that LCMV MX might induce the expression of CA IX (Zavada and Zavadova, 1991). If this were true then the virus infection would represent a potential risk factor for patients with tumors.…”

Section: Lcmv MX Strain and Our Contribution To The Knowledge Of Lcmvmentioning

confidence: 97%

“…It has an extremely restricted host range and is transmissible to HeLa and HEF cells, but not to various human tumor cell lines or animal cell cultures. Notably, some human and animal sera contained antibodies that precipitated from MaTu cell extract, a single protein of 58 kDa (Zavada and Zavadova, 1991).…”

Section: Lcmv MX Strain and Our Contribution To The Knowledge Of Lcmvmentioning

confidence: 99%

Lymphocytic choriomeningitis virus: invisible but not innocent

Laposova¹,

Pastoreková²,

Tomaskova³

2013

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Summary. -Lymphocytic choriomeningitis virus (LCMV) attracts significant attention both as an important experimental model system to study acute and persistent viral infections, and as a neglected human pathogen of clinical significance. This review focuses on the basic aspects and recent advances in the molecular and cell biology of LCMV, the outcome of LCMV infection on its natural host with an emphasis on persistent infection and the outcome of LCMV infection in humans. Lastly, we summarize our contribution to current knowledge on LCM virus.Keywords: lymphocytic choriomeningitis virus; LCMV infection; persistent infection; MX strain * Corresponding author. E-mail: jana.tomaskova@savba.sk; phone: +421-2-50932439. Abbreviations: agRNA = antigenome RNA; α-DG = alpha-dystroglycan; DIP = defective interfering particles; ESCRT = endosomal sorting complex required for transport; GP = glycoprotein; GPC = glycoprotein precursor; IGR = intergenic region; IL-10 = interleukin 10; K1 = keratin 1; LAG-3 = lymphocyte-activation protein 3; LCMV = lymphocytic choriomeningitis virus; MHC = major histocompatibility complex; NP = nucleoprotein; PD-1 = programmed death-1; RNP = ribonucleoprotein; SSP = stable signal peptide; TIM-3 = T cell Ig-and mucin-domain-containing molecule 3; VHF = viral hemorrhagic fever

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“…Human MaTu cells (as described in [12]) and uninfected and LCMV MX-infected cervical carcinoma HeLa cells were cultivated in Dulbecco's Minimal Essential Medium (BioWhittaker, Verviers, Belgium) supplemented with 10% fetal calf serum and 40 lg/ml gentamicin (Lek, Slovenia) in a humidified atmosphere with 5% CO 2 at 37°C.…”

Section: Cell Lines and Virusmentioning

confidence: 99%

“…MX was initially considered an unusual transmissible agent of human carcinoma MaTu cells [12,13]. However, we have shown that this agent is in fact persisting LCMV.…”

Section: Introductionmentioning

confidence: 98%

Molecular characterization of the genes coding for glycoprotein and L protein of lymphocytic choriomeningitis virus strain MX

et al. 2008

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Lymphocytic choriomeningitis virus (LCMV) is the prototype Arenavirus with ambisense coding strategy. We have previously described a new MX strain LCMV and determined the primary structure of the genes coding for the nucleoprotein and RING finger Z protein. In this report, we describe amplification and sequencing of the entire coding sequences of additional MX genes, the glycoprotein precursor (GPC) and L protein. The obtained MX GPC cDNA sequence was 1,615 nucleotides long and contained an ORF, which encodes the GPC precursor of 498 amino acids. MX L polymerase cDNA sequence was 6,668 nucleotides long and predicted ORF encodes the L polymerase of 2,209 amino acids. Nucleotide and deduced amino acid sequences were compared with the known GPC and L sequences and the comparison revealed that both genes shared the highest amino acid identity with Armstrong strain. Phylogenetic analysis confirmed that MX represents a separate LCMV strain. The GPC and L genes products contained several characteristic conserved regions. On the other hand, we have observed numerous differences in predicted protein sequences, which distinguish MX LCMV from other LCMV strains and might be of potential biological significance.

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An unusual transmissible agent ? MaTu

Cited by 11 publications

References 16 publications

Lymphocytic choriomeningitis virus: ways to establish and maintain non-cytolytic persistent infection

Lymphocytic choriomeningitis virus: ways to establish and maintain non-cytolytic persistent infection

Lymphocytic choriomeningitis virus: invisible but not innocent

Molecular characterization of the genes coding for glycoprotein and L protein of lymphocytic choriomeningitis virus strain MX

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