An untargeted metabolomics approach to investigate the wine-processed mechanism of Scutellariae radix in acute lung injury

Hu, Lianqi; Wang, Yaqi; Sun, Haojie; Xiong, You; Zhong, Lei; Wu, Zhenfeng; Yang, Ming

doi:10.1016/j.jep.2020.112665

Cited by 16 publications

(17 citation statements)

References 30 publications

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“…The model group in this study had high levels of retinoic acid rather than the control group, which was consistent with a study observed by Hu et al. ( 2020 ). In addition, thiamine is the core metabolite of the thiamine metabolism pathway, which is closely linked to vitamin B. Thiamine (also known as vitamin B1) has an important role in nerve tissue and mentality and was significantly lower in the model group compared to the control group.…”

Section: Discussionsupporting

confidence: 93%

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Metabolomics coupled with network pharmacology study on the protective effect of Keguan-1 granules in LPS-induced acute lung injury

Chen

Zhou

Zhao

et al. 2022

Pharmaceutical Biology

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Context Keguan-1 (KG-1) plays a vital role in enhancing the curative effects, improving quality of life, and reducing the development of acute lung injury (ALI). Objective To unravel the protective effect and underlying mechanism of KG-1 against ALI. Materials and methods C57BL/6J mice were intratracheally instilled with lipopolysaccharide to establish the ALI model. Then, mice in the KG-1 group received a dose of 5.04 g/kg for 12 h. The levels of proinflammatory cytokines, chemokines, and pathological characteristics were determined to explore the effects of KG-1. Next, untargeted metabolomics was used to identify the differential metabolites and involved pathways for KG-1 anti-ALI. Network pharmacology was carried out to predict the putative active components and drug targets of KG-1 anti-ALI. Results KG-1 significantly improved the levels of TNF-α (from 2295.92 ± 529.87 pg/mL to 1167.64 ± 318.91 pg/mL), IL-6 (from 4688.80 ± 481.68 pg/mL to 3604.43 ± 382.00 pg/mL), CXCL1 (from 4361.76 ± 505.73 pg/mL to 2981.04 ± 526.18 pg/mL), CXCL2 (from 5034.09 ± 809.28 pg/mL to 2980.30 ± 747.63 pg/mL), and impaired lung histological damage. Untargeted metabolomics revealed that KG-1 significantly regulated 12 different metabolites, which mainly related to lipid, amino acid, and vitamin metabolism. Network pharmacology showed that KG-1 exhibited anti-ALI effects through 17 potentially active components acting on seven putative drug targets to regulate four metabolites. Discussion and conclusions This work elucidated the therapeutic effect and underlying mechanism by which KG-1 protects against ALI from the view of the metabolome, thus providing a scientific basis for the usage of KG-1.

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Section: Discussionsupporting

confidence: 93%

“… 2012 ; Hu et al. 2020 ). Tryptophan is converted into several metabolites, such as (indol-3-yl) acetamide and 2-aminophenol, which were significantly increased in the model group compared to the control group.…”

Section: Discussionmentioning

confidence: 99%

Metabolomics coupled with network pharmacology study on the protective effect of Keguan-1 granules in LPS-induced acute lung injury

Chen

Zhou

Zhao

et al. 2022

Pharmaceutical Biology

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“…A metabolomics study showed that the induction of ALI may be related to the sphingolipid metabolism, retinol metabolism, and tryptophan metabolism in LC-MS [ 20 ]. In our study, GC-MS based lung metabolomics was performed to analyze biomarkers and restored metabolic pathways.…”

Section: Discussionmentioning

confidence: 99%

GC-MS Based Metabolomics Reveals the Synergistic Mechanism of Gardeniae Fructus-Forsythiae Fructus Herb Pair in Lipopolysaccharide-Induced Acute Lung Injury Mouse Model

Lin

Yin

et al. 2021

Evidence-Based Complementary and Alternative Medicine

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Compatibility remains among the crucial and significant characteristics of traditional Chinese medicines. The Gardeniae Fructus (FG)-Forsythiae Fructus (FF) herb pair, an epitome of formulations for heat-clearing and detoxification, is extensively used to treat bacterial pneumonia in clinical settings. However, there are few reports on their synergistic effects. This study thus investigated their compatibility by GC-MS based metabolomics using a lipopolysaccharide (LPS)-induced acute lung injury (ALI) mouse model. Differential metabolites were identified by both variable importance in the projection (VIP) > 1 in orthogonal partial least-squares discriminant analysis (OPLS-DA) mode and P < 0.05 . Results of biochemistry and histopathology indicated that FG-FF herb pair exerted more promising lung protective effect than its individual decoction against the LPS-induced ALI model. From the metabolomics study, 32 differential metabolites in vehicle vs. model groups, 21 differential metabolites in FF vs. model groups, 21 differential metabolites in FG vs. model groups, and 20 differential metabolites in FG-FF herb pair vs. model groups were found. Among them, the levels of 3-hydroxybutyric acid, alanine, isophthalic acid, and terephthalic acid were restored significantly in the FF group, while silanol and cholesterol were restored significantly in the FG group. For FG-FF treatment, the amount of behenic acid, a metabolite with anti-inflammatory properties, was increased, while palmitic acid, a proinflammatory metabolite, was decreased. Meanwhile, the two biomarkers were restored more significantly than that by FG or FF treatment, which indicated that the synergistic effects by FF coupled with FG might be attributed to restoring fatty acids metabolic pathway.

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“…Pathway analysis of metabolomics data indicated that CSR acted on acute lung injury by regulating the metabolic pathway of abnormal sphingolipids, but WSR-mediated treatment was related mainly to reversal (to normal) of the abnormality in retinol and tryptophan metabolic pathways. These findings suggested that wine processing changed the mechanism of action of Scutellariae radix against acute lung injury [33]. There are many similar studies on the effect of processing on efficacy of CMM, based on metabolomics.…”

Section: Metabolomics-based Investigaton Of the Effect Of Processing mentioning

confidence: 83%

Current application of metabolomics in the elucidation of processing mechanisms used in Chinese materia medica: A review

Huang¹,

Jiang²,

Zhang³

et al. 2020

Trop. J. Pharm Res

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Processing, a key characteristic of traditional Chinese medicine (TCM), enhances the efficacy and safety of Chinese materia medica (CMM) in clinics. It plays an important role in TCM. Studies on processing mechanisms involved in CMM promote the development of TCM. However, most studies on the mechanisms used for processing CMM do not reflect the holistic theory of TCM because they are based only on analysis of some specific chemical components and biochemical indices which do not support the TCM characteristics of network target and multicomponent therapeutics. Fortunately, the perspective of systems biology is consistent with the holistic theory of TCM. Metabolomics, a key tool in systems biology, has been widely used to investigate the processing mechanism of CMM for many years. In this work, current applications of metabolomics in elucidating mechanisms used for processing of CMM were systematically reviewed and discussed in terms of changes in chemical components, toxicity and efficacy of CMM before and after processing. This work provides researchers a clear and concise reference on the current application of metabolomics in investigation of mechanisms used in processing of CMM. Moreover, this work provides a guide on how to investigate the mechanisms used in processing of CMM, based on metabolomics. Keywords: Chinese materia medica, Processing mechanism, Metabolomics, Holistic theory

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An untargeted metabolomics approach to investigate the wine-processed mechanism of Scutellariae radix in acute lung injury

Cited by 16 publications

References 30 publications

Metabolomics coupled with network pharmacology study on the protective effect of Keguan-1 granules in LPS-induced acute lung injury

Metabolomics coupled with network pharmacology study on the protective effect of Keguan-1 granules in LPS-induced acute lung injury

GC-MS Based Metabolomics Reveals the Synergistic Mechanism of Gardeniae Fructus-Forsythiae Fructus Herb Pair in Lipopolysaccharide-Induced Acute Lung Injury Mouse Model

Current application of metabolomics in the elucidation of processing mechanisms used in Chinese materia medica: A review

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