An Unpublished Hardy Letter

Cell Cycle Regulation of Histone H1 Kinase Activity Associated with the Adenoviral Protein E1A

“…1A). Complexes immunoprecipitated with Pab 416 from HeLa cells infected with wild-type virus had one fifth of the HI kinase of the M73 immune complexes (23). The Hi kinase activity in Pab 416 immune complex from the 293 cells is less than one seventh of that in M73 complex (Fig.…”

“…3B). In addition to p34, the pm928 associated with p60 (cydlin A); however association with pRb was not detected (19,20,23). The histone HI kinase activity precipitated with the antibodies to p60 displays a distinctive pattern of cell cycle regulation in mammalian cells.…”

“…Two of these isoforms were recognized by an antiserum to the COOH-term-inal portion of human p34cdc2 (G6). All four isoforms were recognized by an antibody to S. pombe p34cdc2 (G8) (23) immunoprecipitated with antibody to ElA (anti-ElA) from cells expressing ElA were separated by two-dimensional gel electrophoresis; two spots were detected that appeared to be the same as the two isoforms of p34cdc2 associated with p60 that were not recognized by the G6 antibody but that were bound by the G8 antibody and p13-Sepharose. To (20) and lacks transforming activity (19,21).…”

“…However, the G6 antibody did not immunoprecipitate these forms of p34. ype adenovi-The two isoforms associated with E1A are )32P-labeled present in uninfected HeLa cells and can be the p34Cdc2 recovered by p13-Sepharose from extracts of nmune com-these cells (23). The structure ofthe two p34 the four iso-isoforms associated with p13-Sepharose and clin A) were M73 immune complexes was compared to )r the interval that of p34cdc2 immunoprecipitated with [eLa cells in-G6.…”

Solid-State Chemistry of Ambroxol Theophylline-7-Acetate

“…It shows the expectorant and secretolytic activity of AMB and the bronchodilator activity of the theophylline derivative, making it especially valuable for the treatment of bronchitis, bronchial asthma, emphysema, and chronic obstructive pulmonary disease. 14,15 Regarding the solid-state chemistry of AMB, TAA, and ACE, thus far the polymorphism of both AMB and TAA has been investigated, 16,17 whereas concerning the title compound ACE, only the crystal structures of ambroxol theophylline-7-acetate monohydrate 18 and of a nickel(II) compound containing uncoordinated theophylline-7-acetate ion 19 have been reported. The present paper aims to give a comprehensive picture of the solid-state properties of ACE.…”