An unexpected role for tomato threonine deaminase 2 in host defense against bacterial infection

Yeo, In-Cheol; Manhães, Ana Marcia Escocard de Azevedo; Liu, Jun; Ávila-Pacheco, Julián; He, Ping; Devarenne, Timothy P.

doi:10.1093/plphys/kiac584

Cited by 4 publications

(4 citation statements)

References 85 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Some of these compounds are mentioned to be involved in defence in plants. While L‐threonine itself is not a direct defence compound, the enzyme threonine deaminase converts threonine to α‐ketobutyrate and ammonia as the committed step in isoleucine (Ile) biosynthesis and contributes to JA responses by producing the Ile needed to make the bioactivate JA‐Ile conjugate, important for defence against necrotrophs (Gonzales‐Vigil et al, 2011; Yeo et al, 2023). 1‐Aminocyclopropanecarboxylic acid (ACC) acts as the direct precursor of the plant hormone ET, which regulates plant growth and biotic and abiotic stress responses (Van de Poel & Van Der Straeten, 2014; Zaynab et al, 2018).…”

Section: Resultsmentioning

confidence: 99%

“…While L-threonine itself is not a direct defence compound, the enzyme threonine deaminase converts threonine to α-ketobutyrate and ammonia as the committed step in isoleucine (Ile) biosynthesis and contributes to JA responses by producing the Ile needed to make the bioactivate JA-Ile conjugate, important for defence against necrotrophs (Gonzales-Vigil et al, 2011;Yeo et al, 2023).…”

Section: Candidate Metabolites Associated With Chitin and A Alternata...mentioning

confidence: 99%

See 1 more Smart Citation

Untargeted metabolomics reveals PTI‐associated metabolites

Muñoz Hoyos,

Anisha,

Meng

et al. 2024

Plant Cell & Environment

View full text Add to dashboard Cite

Plants employ a multilayered immune system to combat pathogens. In one layer, recognition of Pathogen‐ or Microbe‐Associated Molecular Patterns or elicitors, triggers a cascade that leads to defence against the pathogen and Pattern Triggered Immunity. Secondary or specialised metabolites (SMs) are expected to play a role, because they are potentially anti‐fungal compounds. Tomato (Solanum lycopersicum) plants inoculated with Alternaria solani s.l. show symptoms of infection after inoculation. Plants inoculated with Alternaria alternata remain symptomless. We hypothesised that pattern‐triggered induction of resistance related metabolites in tomato contributes to the resistance against A. alternata. We compared the metabolomic profile (metabolome) of tomato after treatments with A. alternata, A. solani and the fungal elicitor chitin, and identified SMs involved in early defence of tomato plants. We revealed differential metabolome fingerprints. The composition of A. alternata and chitin induced metabolomes show larger overlap with each other than with the A. solani induced metabolome. We identify 65 metabolites possibly associated with PTI in tomato plants, including NAD and trigonelline. We confirm that trigonelline inhibits fungal growth in vitro at physiological concentrations. Thus, a true pattern‐triggered, chemical defence is mounted against A. alternata, which contains anti‐fungal compounds that could be interesting for crop protection strategies.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Candidate Metabolites Associated With Chitin and A Alternata...mentioning

confidence: 99%

Untargeted metabolomics reveals PTI‐associated metabolites

Muñoz Hoyos,

Anisha,

Meng

et al. 2024

Plant Cell & Environment

View full text Add to dashboard Cite

show abstract

“…Some of these compounds are mentioned to be involved in defence in plants. While L-threonine itself is not a direct defence compound, the enzyme threonine deaminase (TD) converts threonine to α-ketobutyrate and ammonia as the committed step in isoleucine (Ile) biosynthesis and contributes to JA responses by producing the Ile needed to make the bioactivate JA-Ile conjugate, important for defence against necrotrophs (Gonzales-Vigil et al, 2011;Yeo et al, 2023). 1-Aminocyclopropanecarboxylic acid (ACC) acts as the direct precursor of the plant hormone ethylene, which regulates plant growth and biotic and abiotic stress responses (Van de Poel & Van Der Straeten, 2014;Zaynab et al, 2018).…”

Section: Candidate Metabolites Associated With Chitin and A Alternata...mentioning

confidence: 99%

Untargeted metabolomics reveals PTI-associated metabolites in tomato

Hoyos

Wan

Meng

et al. 2023

Preprint

View full text Add to dashboard Cite

Plants employ a multi-layered innate immune system to detect and fend off invading fungal pathogens. In one such layer, recognition of Pathogen- or Microbe-Associated Molecular Patterns or elicitors, triggers a signaling cascade that leads to defence against the pathogen and ultimately Pattern-Triggered Immunity (PTI). Secondary Metabolites (SMs) are expected to play an important role in this kind of resistance, because they are potentially mycotoxic compounds. Tomato plants inoculated with Alternaria solani show clear symptoms of infection 5 days after inoculation. Whereas plants inoculated with Alternaria alternata remain symptomless. We hypothesized that pattern-triggered induction of resistance-related metabolites in Solanum lycopersicum contribute to the resistance against A. alternata, yet such SMs are suppressed in a compatible interaction. We compared the metabolomic profile (metabolome) of S. lycopersicum at two time points (3 and 24 hours) after treatments with A. alternata, A. solani and the fungal elicitor chitin and identified SMs that are involved in the early defence response of tomato plants. Our study revealed differential metabolome fingerprints and shows that the molecular composition of A. alternata and chitin-induced indeed show larger overlap with each other than with the A. solani-induced metabolome. We identify 65 candidate metabolites possibly associated with pattern-triggered resistance in tomato plants, including the alkaloid, trigonelline, for which we can confirm that it inhibits fungal growth in vitro when supplied at physiological concentrations. Our findings show that a true, pattern-triggered, chemical defence is mounted against A. alternata and that it contains mycotoxin compounds previously unidentified in tomato, that could be interesting for future crop protection strategies.

show abstract

“…JA synthesis genes affected crops resistance to B. cinerea [78,79]. Tomato SlTD2 is a threonine deaminase that converts threonine to α-ketobutyrate and ammonia as the committed step in isoleucine (Ile) biosynthesis and contributes to JA responses by producing the Ile needed to make the bioactive JA-Ile conjugate [80]. SlBBX20 attenuates JA signaling and regulates resistance to B. cinerea in tomato [81].…”

Section: Jasmonic Acidmentioning

confidence: 99%

Recent Advances in Mechanisms Underlying Defense Responses of Horticultural Crops to Botrytis cinerea

Li,

Cheng

2023

Horticulturae

View full text Add to dashboard Cite

Horticultural crops are a crucial component of agriculture worldwide and have great economic value. The notorious plant fungal pathogen Botrytis cinerea can cause gray mold disease in over 200 horticultural crops, leading to severe economic losses. Investigating the mechanisms underlying plant defense responses to pathogens is crucial for developing new strategies for effectively controlling plant diseases, and much progress has occurred in the mechanisms underlying defense responses of horticultural crops to B. cinerea mainly due to the completion of genome sequencing and the establishment of efficient tools for functional genomics. In this review, recent progress in mechanisms underlying defense responses and natural products that can enhance the resistance of horticultural crops to B. cinerea are summarized, and future research directions are also discussed.

show abstract

An unexpected role for tomato threonine deaminase 2 in host defense against bacterial infection

Cited by 4 publications

References 85 publications

Untargeted metabolomics reveals PTI‐associated metabolites

Untargeted metabolomics reveals PTI‐associated metabolites

Untargeted metabolomics reveals PTI-associated metabolites in tomato

Recent Advances in Mechanisms Underlying Defense Responses of Horticultural Crops to Botrytis cinerea

Contact Info

Product

Resources

About