Chalcones constitute an important group of natural products, some of which possess a wide range of biological properties, such as antibacterial, antifungal, anti-inflammatory, antitumour and antimicrobial. [1][2][3][4][5] The literature survey has shown only a few reports on the sulfur-containing chalcone derivatives, which showed antiviral and antibacterial properties. 6,7 The mass spectral fragmentation of various substituted derivatives of chalcone has previously been studied. [8][9][10][11][12][13] The main aim of the present work was to establish the mass spectrometric fragmentations of new (E)-4-chalconylothioalkyl-substituted derivatives of piperidinie (5-8), 4-methylpiperidine (9-12), morpholine (13-16) and piperazine (17-20) as well as (E)-4-bromoalkylthiochalcones (1-4) being substrates of the former compounds ( Figure 1). This investigation was also undertaken to find out whether it is possible to differentiate between the isomeric N-(E)-chalconylothioalkyl-substituted piperidines (6,7) and N-(4-methyl)-piperidines (9,10) on the basis of electron impact (EI) mass spectrometric analysis.The low-resolution EI mass spectra (Tables 1-3), as well as B/E and B 2 /E-linked scan spectra and exact mass determinations, permitted identification of the principal mass fragmentation routes of compounds 1-20.The common feature of fragmentation of the molecular ions of (E)-4-bromoalkylthiochalcones 1-4 is a simple cleavage of the C sp3 -Br bond in the bromoalkylthio substituent with elimination of the